Copper ion/mercury ion fluorescence molecular probe, and preparation method and application thereof
A technology of fluorescent molecular probes and copper ions, applied in chemical instruments and methods, fluorescence/phosphorescence, luminescent materials, etc., can solve the problems of single detection ions, sensitivity to pH value, low sensitivity, etc., and achieve high fluorescence quantum yield , small detection interference, good chemical/light stability
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0041] 1) Dissolve 1 kg of rhodamine B in 30 kg of ethanol, add 2 kg of hydrazine hydrate at room temperature, stir and reflux for 1 hour, cool, and filter the precipitate;
[0042] 2) Get 1 kilogram of precipitate and add 50 kilograms of the mixed solvent of methanol / dichloromethane with a volume ratio of 3:1, then add 2 kilograms of formaldehyde solution with a mass fraction of 40%, and stir at room temperature for 6 hours under nitrogen protection. The solvent was evaporated under pressure, and the fluorescent molecular probe was obtained by silica gel chromatography.
[0043] Elemental Analysis C 29 h 32 N 4 o 2 C, 74.33; N, 11.96; Experimentally measured: H, 6.93; C, 74.26; N, 11.63; In deuterated chloroform, proton nuclear magnetic resonance spectrum signal is (chemical shift, unit ppm): 7.91 ( d, J=7.2Hz, 1H), 7.46-7.49(m, 2H), 7.05(d, J=7.6Hz, 1H), 6.48-6.52(m, 4H), 6.25(s, 2H), 3.68(s , 2H), 3.37(q, J=7.2Hz, 8H), 1.13(t, J=7.2Hz, 12H); the electrospray soft ioniz...
Embodiment 2
[0048] 1) Dissolve 1 kg of rhodamine B in 40 kg of ethanol, add 4 kg of hydrazine hydrate at room temperature, stir and reflux for 1.5 hours, cool, and filter the precipitate;
[0049] 2) Take 1 kg of precipitate and add 200 kg of methanol / dichloromethane with a volume ratio of 3:1 in a mixed solvent, then add 4 kg of formaldehyde solution with a mass fraction of 50%, and stir at room temperature for 7 hours under nitrogen protection. The solvent was evaporated under pressure, and the fluorescent molecular probe was obtained by silica gel chromatography.
[0050] application:
[0051] Copper ion content detection: Mix water and acetonitrile evenly according to the volume ratio of 3:1, let stand at room temperature for 30 minutes, then add the molecular probe of this example into the above mixed solution, and make the concentration 1 μM to make a standard Solvent, and then use the prepared standard solvent to prepare copper ion standard solutions with concentrations of 0, 5, 1...
Embodiment 3
[0054] 1) Dissolve 1 kg of rhodamine B in 50 kg of ethanol, add 5 kg of hydrazine hydrate at room temperature, stir and reflux for 2 hours, cool, and filter the precipitate;
[0055] 2) Take 1 kilogram of precipitate and add 500 dry grams of methanol / dichloromethane in a mixed solvent with a volume ratio of 3:1, then add 6 kilograms of formaldehyde solution with a mass fraction of 60%, and stir at room temperature for 8 hours under nitrogen protection. The solvent was evaporated under reduced pressure, and the fluorescent molecular probe was obtained by silica gel chromatography.
[0056] application:
[0057] Copper ion content detection: Mix water and acetonitrile evenly according to the volume ratio of 9:1, let stand at room temperature for 60 minutes, then add the molecular probe of this example into the above mixed solution, and make the concentration 1 μM to make a standard Solvent, and then use the prepared standard solvent to prepare copper ion standard solutions with...
PUM
Property | Measurement | Unit |
---|---|---|
quality score | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com