56results about How to "Detection interference is small" patented technology

The invention relates to a copper ion / mercury ion fluorescence molecular probe, and a preparation method and application thereof. The invention mainly aims to solve the problem of single detection ion in the fluorescence molecular probe for detecting copper ions or mercury ions in the prior art, and the problems of low sensitivity, poor selectivity, high sensitivity to pH value, and the like in most fluorescence molecular probes. The preparation method of the fluorescence molecular probe comprises the following steps: (1) dissolving 1 part of rhodamine B in ethanol, adding 2-5 parts of hydrazine hydrate at room temperature, stirring under reflux for 1-2 hours, cooling, and filtering to obtain a precipitate; and (2) adding 1 part of precipitate into 50-500 parts of mixed solvent composed of methanol and dichloromethane in a volume ratio of 3:1, adding 2-6 parts of 40-60wt% formalin, stirring at room temperature in a nitrogen atmosphere for 6-8 hours, evaporating under reduced pressure to remove the solvent, and carrying out silica gel chromatographic separation to obtain the fluorescence molecular probe. The invention has the advantages of high fluorescence quantum yield, low sensitivity to solvent polarity, high chemical / light stability and the like.

The invention discloses an ESPR-based heavy metal analyzer, which comprises a chemical reaction part, a surface plasma resonance spectrometer and an information processing system, wherein the chemical reaction part includes a detection cell, a counter electrode, a reference electrode, an array electrode and an electrochemical workstation; the array electrode is positioned at the bottom of the detection cell, the counter electrode and the reference electrode are inserted in the detection cell, the counter electrode, the reference electrode and the array electrode are respectively connected to the electrochemical workstation; the electrochemical workstation and the surface plasma resonance spectrometer are respectively connected to the information processing system. The invention further discloses an analysis method of the ESPR-based heavy metal analyzer, which achieves the simultaneous detection of multi-objective heavy metals, enhances the detection sensitivity and spatial resolution, and reduces damages on thin metallic membrane plate (the array electrode and active points thereof).

The invention discloses a traffic sign detecting method based on a self-adaptation threshold value. Firstly, a reddening and bluing method is adopted by the traffic sign detecting method to carry out image preprocessing, a red-blue grayscale image is obtained, and then multiple times of thresholding processing are carried out on the grayscale image. During each time of thresholding processing, contour detection is carried out on a binary image generated by the thresholding processing; and then, according to shape characteristics of a traffic sign, sign contour matching with geometric condition constraint and Hu invariant moment is carried out, and after filtering and screening are carried out, a suspected traffic sign regional set in a current threshold image is obtained; finally, results of the multiple times of thresholding processing are merged, and according to the frequency for detecting of contour regions of the sign, interest regions of the traffic sign are finally confirmed. According to the traffic sign detecting method, a better thresholding processing effect is provided for candidate areas of the traffic sign in the image, the feature of threshold value self-adaptation is provided, the efficient detecting efficiency and the time performance are provided, and the problem of adaptability under different illuminating conditions is excellently solved.

The invention provides a docking ship detection method based on edge line analysis and aggregation channel characteristics. The method comprises the following steps: firstly, realizing sea-land segmentation based on multi-scale multi-structure element morphological filtering, defining an edge point gradient tangent angle and port convexity characteristics by combining rectangular shape characteristics of a port in a remote sensing image to position the port, and obtaining a port region-of-interest set; and then, extracting aggregation channel characteristics of the ship target, constructing aship sample training library based on the aggregation channel characteristics, finishing training of a classifier by utilizing an AdaBoost algorithm, and finishing final judgment and confirmation of the ship target by utilizing the trained classifier. According to the invention, the port can be quickly positioned from the large-scale remote sensing image; port positioning does not need to acquiregeographic prior information and perform a large amount of feature matching calculation, ship features are fully reflected by utilizing aggregation channel features, the problem of serious interference of the backgrounds of ports on ship detection is solved, various types of ships can be detected, and the application range is wide.

The invention relates to a copper ions detection method based on nano-cluster. The method is characterized by comprising the following steps: 1) mixing a standard copper ions solution and gold nanoclusters, establishing a standard curve of fluorescence signal quenching strength for detecting the nano-cluster and copper ions amount; and 2) mixing a sample solution and the gold nanoclusters, bring the fluorescence signal quenching strength for detecting the nano-cluster into the standard curve to obtain the content of copper ions in the sample. The concentration of the gold nanoclusters is 0.5 mM, a pH value is 7; and the gold nanoclusters are coated and modified by GSH. The experiment proves that the sensitivity of the detection method is high, detection interference of other ions to the copper ions is less, the method can realize selectivity detection to the copper ions, so that a purpose for rapidly detecting the concentration of copper ions can be reached, and the method has wide application prospect for copper ion detection.

This invention discloses one filter property online test method and device, which comprises the following steps: using one probe under test area and pressure to test filter transparent degree and liquid content; transparent degree test is through filter liquid flow and pressure; liquid content is to use liquid device for test through controller to convert device signal into liquid content. The test device comprises probe, spray head, liquid device with probe, pressure sensor, flow sensor, pressure adjust valve, pressure tar, controller and power, wherein, the spray head and liquid detect probe are fixed onto probe; the spray head is connected with pressure sensor and flow sensor through pressure adjust valve and pressure tar.

The invention discloses a packing tower flooding prediction method based on pressure drop standard deviation analysis. The method includes 1, arranging pressure drop detection ports of packing layers of a packing tower; 2, allowing an intelligent pressure transmitter to connect to the pressure drop detection ports through pressure detection pipes and perform detection in real time; 3, allowing the pressure drop detection ports to transmit the pressure drop data to a data collector, processing the data, and acquiring the changing curve of pressure drop with tower operation flux and the pressure drop standard deviation distribution curve; 4, adopting the pressure drop standard deviation distribution curve as the basis for judging the packing tower flooding, and determining the flooding state when the slope of the pressure drop standard deviation distribution curve exceeds a critical value. The acquiring frequency of the pressure drop detection ports is larger than 20 hertz.

The invention relates to a fluorescent molecular probe for detecting Cu<2+> in the field of fine chemical engineering, in particular to a preparation method of a Cu<2+> fluorescent molecular probe based on rhodamine dyes and an application of the fluorescent molecular probe to biocellular imaging and water system copper ion detection. A preparation method of a compound I includes the steps: adding 1 part of (E)-2-((3', 6'-bis (diethylamino)-3-oxaspiro [isoindazole-1, 9'-xanthene]-2-alkene) imine) ethylal and 6 parts of hydrazine hydrate into a reaction vessel, heating and stirring mixture, performing rotary evaporation to remove ethyl alcohol after reaction, and adding 50 parts of methylene dichloride and 150 parts of saturated sodium chloride solution; separating mixture by the aid of a separating funnel, drying a organic phase by the aid of anhydrous sodium sulfate, performing spin drying on solvents, and separating coarse products by a column chromatography to obtain the compound I. The preparation method is mild in reaction condition, less in synthesis step and high in product yield, and raw materials are low in cost and easily acquired.

The invention discloses a graphene-modified lactic acid biosensor. The graphene-modified lactic acid biosensor comprises a substrate, a reference electrode, a counter electrode, a working electrode and an insulating layer, wherein the reference electrode, the counter electrode and the working electrode are parallely arranged on the substrate; the insulating layer partially coats the surfaces of the reference electrode, the counter electrode and the working electrode; the insulating layer and the exposed parts of the reference electrode, the counter electrode and the working electrode define a reaction chamber; the working electrode comprises a carbon paste lead, a graphene layer and an enzyme liquid layer; the graphene layer covers a reaction zone of the carbon paste lead; and the enzyme liquid layer is located in the reaction chamber and bonded with the graphene layer and contains lactic acid oxidase and an enzyme active additive.

The invention discloses a detection method for selectively recognizing the Fe3+ capacity. The method comprises the following steps of respectively preparing a standard solution of acetonitrile with the concentration of 2*10<-5>mol / L of Ag+, Ca2+, Cd2+, Co2+, Cr3+, Cu2+, Fe3+, Hg+, Hg2+, K+, Mg2+, Mn2+, Na+, Ni2+, Pb2+ and Zn2+, and arranging 1ml of the standard solution into a cuvette; adding a probe solution into each cuvette, standing the cuvette, wherein the cuvette with Fe3+ is rapidly changed to be colorless. By adopting the method, not only can the F3+ be recognized in an organic solvent, but also the Fe3+ can be sensitively recognized in the buffering aqueous solution, the CN- with high toxicity can be recognized on the basis, and the fluorescent probe after being processed by utilizing the method can be primarily used for fluorescently recognizing Fe3+ in a cell body.

The invention relates to a surface molecular imprinting SERS (Surface Enhanced Raman Scattering) sensor based on core-shell structure polydopamine coated gold nanoparticles as well as preparation andapplication of the surface molecular imprinting SERS sensor. A preparation method comprises the following steps: by taking dopamine as a functional monomer and a phthalate substance as a template molecule, carrying out self-polymerization on the surfaces of gold nanoparticles to obtain a molecularly imprinted polymer, eluting the molecularly imprinted polymer, and dropwise coating the surface of ascreen-printed electrode with the molecularly imprinted polymer to obtain the surface molecularly imprinted SERS sensor. Compared with the prior art, the invention is combined with a Raman spectrometer, by combining electrochemical enrichment and molecular imprinting technologies, high-selectivity and high-sensitivity detection of phthalate substances in a water body in a complex environment canbe realized at the same time, and the sensor has the advantages of simple preparation process, high detection speed, no need of sample pretreatment, environmental friendliness, low cost and the like,and shows a wide application prospect.

The invention provides an egg cooker and a control method for the egg cooker. The egg cooker comprises an egg cooking rack provided with through holes, heaters, a heating chamber, a cover and a controller; the egg cooker further comprises an ultrasonic transmitter arranged on the cover and an ultrasonic receiver arranged on the egg cooking rack, and the controller is respectively connected with the heaters, the ultrasonic transmitter and the ultrasonic receiver. The control method comprises the steps of S100, transmitting an ultrasonic signal and acquiring measurement information; S200, detecting raw and cooked state information of egg food in the egg cooker according to the measurement information; S300, comparing the raw and cooked state information with a preset raw and cooked state, and controlling the working state of the egg cooker according to the comparison result. According to the egg cooker and the control method, the automatic recognition of the degree of cooking of the eggfood is achieved, and the automatic stopping of heating is achieved according to the degree of cooking.

The invention discloses a method for quantitatively detecting a free amino acid in a clinical sample, and belongs to the field of analysis and detection. According to the method, an acidic methanol solution is used for extracting the free amino acid in the clinical sample, then the property of the amino acid is changed through alcohol derived carboxyl, and deuteration agent derived amino acid serves as an internal standard instead of deuteration amino acid to be favorable for chromatographic separation and quantitative mass spectrometric detection. The operation of the method is simple, the amino is basically separated within a short period of time, the interference of a matrix is greatly reduced, the cost is low, the specificity is good, and the method is suitable for quantitative detection of the free amino acid in the clinical sample.

The invention provides a biosensor and a preparation method thereof, and the biosensor comprises an electrode structure which comprises a substrate, a first conductive layer, a first insulating layer, a second conductive layer, a third conductive layer and a second insulating layer, wherein the first conductive layer is formed on the substrate; the first insulating layer is formed on the first conducting layer, and a part of area of the first conducting layer is exposed to form a working electrode; the second conductive layer is formed on the first insulating layer; the third conductive layer is formed on a partial region of the second conductive layer; the second insulating layer is at least formed on the second conducting layer, a part of area of the second conducting layer is exposed to form a counter electrode, and at least a part of area of the third conducting layer is exposed to form a reference electrode; and the reaction film layer is formed on the working electrode and is used for carrying out electrochemical reaction with a target object. The biosensor is simple and reasonable in structure and beneficial to batch production.

The invention discloses a wafer caching device and a chemical mechanical polishing system. The wafer caching device comprises a support frame and a detection assembly, the support frame is used for horizontally supporting a wafer, and the detection assembly is arranged at the peripheral side of the support frame; the detection assembly comprises a signal receiving and transmitting part and a reflection part arranged in a matched mode, an optical signal output by the signal receiving and transmitting part is emitted towards the reflection part, and the reflection part reflects the optical signal to the signal receiving and transmitting part; and the detection assembly judges whether the wafer is placed on the support frame or not according to the optical signal received by the signal receiving and transmitting part.

The invention discloses a free fatty acid detection kit and a preparation method thereof. The kit comprises reagents A and B, wherein the reagent A is prepared from the following components: sodium dihydrogen phosphate, a coenzyme A, triphosadenine, acyl coenzyme A synthetase, MgCl2, Trinder substrate and a proper amount of magnetized water, and the pH value is regulated to 7.2. The reagent B is prepared from the following components: sodium dihydrogen phosphate, 4-aminoantipyrine, acyl coenzyme A oxidase, peroxidase, flavin adenine dinucleotide, EDTA-2Na, N-sodium cocoanutylglutamate, sodiumlauryl ether sulphate, sodium N-acyl-L-serine, mannitol, sodium citrate and a proper amount of magnetized water, and the pH value is regulated to 7.2. The kit has the advantages of high and stable enzymatic activity and long preservation life; after the kit is preserved for about 15 days at 35-37 DEG C, the deviation of the detection result is only 1.99%; and the sensitivity is improved by 3.45%.

The invention belongs to the technical field of chemical element detection, and provides a method for detecting zinc in dust mud based on atomic absorption spectrometry, which is less in detection interference, high in accuracy, low in detection limit, high in detection speed, capable of detecting samples with higher content through a dilution method, and suitable for detecting samples with complex dust mud components. The method can simplify the detection means of the zinc element in the dust mud, reduces reagents used for detection, improves the efficiency, and is a simple and efficient detection method.

The invention discloses a traffic sign detection method based on an adaptive threshold. The present invention first adopts the red and blue method to preprocess the image to obtain the red and blue gray scale image, and then performs multiple threshold value processing on the gray scale image. In each thresholding process, contour detection is performed on the binary image generated by the thresholding process; then, according to the shape characteristics of the traffic sign, geometric constraints and sign contour matching of Hu invariant moments are performed, and the current threshold image is obtained by filtering and screening. The set of suspected traffic sign areas; finally, the multiple thresholding results are combined, and the final traffic sign ROI is determined according to the frequency of detection of the outline area of ​​the sign. The present invention enables the traffic sign candidate area in the image to obtain a better threshold processing effect, has the characteristics of threshold self-adaptation, not only has high detection efficiency and time performance, but also well solves the adaptability problem under different lighting conditions .

The invention discloses an acid rain total-analysis online monitoring system which comprises a rainwater collecting device, a rainwater measuring device, a rainwater sample reserving device, a pipeline cleaning device, a control device and a sample separating unit with a rainwater metering function. The sample separating unit with the rainwater metering function comprises a metering bottle; A main flow dividing pipe at the lower end of the metering bottle is communicated with a measuring groove of the pH value and conductivity measuring unit through a first flow dividing pipe, is communicated with the ion measuring unit through a second flow dividing pipe, is communicated with the rainwater sample reserving device through a third flow dividing pipe; the metering bottle comprises a 35cc metering bottle and a first positioning electrode detection bottle; and a reflux pipe communicated with the 35cc metering bottle is also arranged in the 260cc reserved sample temporary storage bottle of the rainwater sample reserving device. According to the acid rain total-analysis online monitoring system, the monitoring interference caused by corrosion or electrolysis of the positioning detection electrode can be avoided, monitoring interference caused by residual liquid in a container and a pipeline is reduced, and detection analysis deficiency when rainfall is insufficient is made up.

The invention discloses an optical inspection apparatus and an optical inspection method. The optical inspection apparatus comprises a first detection device, a second detection device, and a sensing device. The first detection device is composed of a first display device and a first control device. The first control device is connected with the first display device. The first control device is used for controlling the state of the first display device. The second detection device is composed of a second display device and a second control device. The second control device is connected with the second display device. The second control device is used for controlling the state of the second display device. The first display device and the second display device are oppositely arranged. The sensing device is used for sensing the movement of a target position and sending signals to the first control device and the second control device. The sensing device is arranged between the first detection device and the second detection device. According to the optical inspection apparatus, the number of configured people can be reduced, and a large amount of mobile consumption is avoided. Meanwhile, the automatic switching among light sources is realized, and the detection interference is reduced.

The invention provides a four-pin dual-working high-precision photoelectric transparent interdigital electrode containing an insulating protective film and application of the four-pin dual-working high-precision photoelectric transparent interdigital electrode in photoelectric joint detection.The four-pin dual-working high-precision photoelectric transparent interdigital electrode comprises an electrode substrate, an electrode area is arranged at one end of the front face of the electrode substrate, and a pin area is arranged at the other end of the front face of the electrode substrate; the electrode area comprises a first detection part (1), a second detection part (2), a third detection part (3) and a fourth detection part (4), the third detection part (3) is located on the right side of the first detection part (1), the second detection part (2) is located between the first detection part (1) and the third detection part (3), and one part of the fourth detection part (4) is located on the right side of the third detection part (3); the other part is located on the outer side of the first detection part (1) and the third detection part (3), the first detection part (1) and the third detection part (3) vertically extend to form a first interdigital strip and a second interdigital strip which are crossed and parallel, the first interdigital strip and the second interdigital strip are arranged in a staggered mode in a tooth comb shape, the first interdigital strip is arranged in a gap of the second interdigital strip, and the first interdigital strip and the second interdigital strip are arranged at the same interval. The electrode can be used for electrochemical detection of extremely small amounts of reagents.

The invention relates to the technical field of gas detection, in particular to a coal seam gas pressure and concentration integrated detection device which is internally provided with a gas pressure detection module, a gas concentration detection module and a bag body driving and controlling module, an outer sleeve is fixedly arranged on one side of the coal seam gas pressure and concentration integrated detection device and provided with a plurality of expansion bag bodies, and an inner sleeve is fixedly arranged on the inner periphery of the outer sleeve. A gas pressure guide pipe and a gas concentration guide pipe are arranged on the inner periphery of the inner sleeve. The gas pressure guide pipe is connected with the detection pipeline of the gas pressure detection module; and the gas concentration conduit is connected with the detection pipeline of the gas concentration detection module. The expansion bag body comprises an outer side bag, the inner periphery of the outer side bag is connected with a set of fixing frames, and the inner peripheries of the set of fixing frames are jointly connected with an inner side soft ring. The gas pressure detection module and the gas concentration detection module are arranged at the detection terminal, and the gas pressure and the gas concentration are independently detected by adopting double independent pipelines, so that the detection interference is reduced, and the device can be quickly disassembled and reused.

The invention relates to an excitation-shielding quantitative detection probe and a corresponding method. The probe comprises a rectangle constant permanent magnet, an L-shaped shielding element and a magneto sensor. The L-shaped shielding element is attached to an S-pole part of a horizontal side-face and a non-working-face of the rectangle constant permanent magnet; the magneto sensor is arranged on a magnetic-field zero-intensity position of a mutual zone between the N-pole and S-pole on the non-working-face of the rectangle constant permanent magnet. The method includes the following steps: a magnetic field intensity initial value of a magnetometer is set; the working face of the probe is led to touch an outer wall of a detected austenitic steel pipe and to move along the length of the steel pipe; both a position on the steel pipe where the detected magnetic field intensity is the maximum and a position on the steel pipe where the detected magnetic field intensity is reduced to aninitial value from the maximum are recorded; the probe is moved along the circle of the steel pipe and a position on the steel pipe where the magnetic field intensity is reduced to the initial value is recorded. For the invention, the size of the probe is small so as to reduce the interference to the detection by the accumulated oxide film in the austenitic steel pipe. The invention can be widelyapplied to the detection to boilers of power plants.

The invention relates to a method of reducing lipid blood sample detection interferences. The method includes a step of preparing a lipase buffer solution, namely a step of weighing 1-2.5 g of lipase and 1-2 g of auxiliary lipase, measuring 4-10 mL of a dipotassium phosphate-monopotassium phosphate buffer liquid, and fully mixing and dissolving the lipase, the auxiliary lipase and the buffer liquid in a glass container, with the lipase activity of the prepared solution being higher than 500 [mu] / L; a step of adding 5-10% of the prepared lipase buffer solution into a lipid blood sample and performing incubation for 10-20 min; and a step of performing centrifugal treatment by adopting a centrifuge, with the rotating speed of the centrifuge being controlled to be 1800-3000 r / min. The method does not need a high-requirement expensive supercentrifuge, a detecting process is simple and a detection result is small in error.

The invention provides a method for preparing a fluorescent probe for iodide ion detection in water samples, which is characterized in that it comprises: step 1: after mixing phosphorus pentoxide solid, sodium hydroxide solid and N-methylpyrrolidone Ultrasound at room temperature, put the ultrasonic reaction solution in an oil bath, and prepare phosphorus oxide quantum dots by solvothermal method; the reaction solution is centrifuged to remove the precipitate, the supernatant is taken, and purified by dialysis, and the dialysate After concentration and freeze-drying, a blue-green fluorescent phosphorus oxide quantum dot solid material is obtained; step 2: dissolving the blue-green fluorescent phosphorus oxide quantum dot solid material in deionized water to obtain a phosphorus oxide quantum dot aqueous solution, and the phosphorus oxide quantum dot aqueous solution and Ag + Aqueous solutions are mixed to obtain Ag + The phosphorous oxide quantum dot probe mediated by the method is a fluorescent probe for the detection of iodide ions in water samples. The fluorescent probe obtained by the invention can quickly detect iodide ions, and the probe has better selectivity for iodide ions in different water samples.