The invention provides a method for extracting and determining tea
polyphenol in a tobacco extract. The method for extracting and determining the tea
polyphenol in the tobacco extract comprises the following steps: preparing a
standard solution of a tea
polyphenol substance; adding a
methanol aqueous solution into the tobacco extract, and fixing the volume to obtain a tea polyphenol
diluent; sealing the tea polyphenol purified liquid, and performing ultrasonic extraction to obtain a tea polyphenol extracting solution; preparing a disodium
ethylene diamine tetraacetate stabilizing solution, and fixing the volume of the tea polyphenol extracting solution by using the disodium
ethylene diamine tetraacetate stabilizing solution to obtain a tea polyphenol purified solution; adding a PSA adsorbent into the tea polyphenol purified liquid, carrying out centrifugal standing to obtain a to-be-detected liquid, and carrying out instrumental analysis on the to-be-detected liquid. Through ultrasonic extraction, the loss of tea polyphenol substances is less, the preparation of a to-be-detected solution is simple, and the analysis time is short; by adding the disodium
ethylene diamine tetraacetate and the PSA adsorbent, the interference of other substances on the detection of the tea polyphenol substances is reduced, so that the detection of the tea polyphenol substances in the tobacco extract is more accurate.