Method for detecting trace amounts of Ca<2+>, Sr<2+> and Ba<2+> in active cancer cells through single-channel fluorescence imaging

A fluorescence imaging, single-channel technology, used in measurement devices, fluorescence/phosphorescence, material analysis by optical means, etc., can solve problems such as poor selectivity, inconvenient detection, and inability to achieve efficient detection, and achieve convenient monitoring and high sensitivity. , the effect of less interference
CN108548804AActive Publication Date: 2018-09-18GUIZHOU UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
GUIZHOU UNIV
Publication Date
2018-09-18

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Abstract

The invention discloses a method for detecting trace amounts of Ca<2+>, Sr<2+> and Ba<2+> in active cancer cells through single-channel fluorescence imaging. By using classic calixarene [4] derivativeas a probe, and the trace amounts of Ca<2+>, Sr<2+> and Ba<2+> in the active cancer cells are respectively detected through the single-channel fluorescence imaging. According to the method disclosedby the invention, detection on multi-target ions can be realized; a coordination compound of a probe-Ca<2+>, a probe-Sr<2+> and a probe-Ba<2+> can be respectively formed by the probe disclosed by theinvention and Ca<2+>, Sr<2+> and Ba<2+> in the cells, and intense fluorescence with different wavelengths can be emitted, so that detection is conveniently carried out, the visibility is good, the sensitivity is good, the selectivity is good, and real-time on-line detection of the Ca<2+>, Sr<2+> and Ba<2+> in the cells can be realized.
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Description

technical field

[0001] The invention relates to a single-channel fluorescence imaging method for detecting trace ions in living cells, in particular to a single-channel fluorescence imaging method for detecting trace amounts of Ca in active cancer cells 2+ 、Sr 2+ and Ba 2+ Methods. Background technique

[0002] In cell biology and medical research, fluorescent probes are the most powerful tools for allowing researchers to monitor events in living cells and living organisms in real time due to their high spatial resolution and minimal disturbance of the living body. One of them is being paid more and more attention to and used. Probes are particularly important for the study of metal ion transport carriers and the crossing behavior of cell membranes in drug delivery systems. Fluorescence imaging to detect intracellular ions can provide the most direct information on the temporal and spatial distribution of ions in living systems. Due to its non-invasive, high sensitivity,...

Claims

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