Preparation method of polyisoacrylamide sphere modified fluorescent silicon dot, prepared fluorescent silicon dot and application of fluorescent silicon dot

A technology of acrylamide balls and silicon dots, which is applied in the field of fluorescent silicon dots, achieves the effects of solving poor biocompatibility and poor selectivity of temperature-sensitive detection of cancer cells, being easy to produce on a large scale, and having a simple and easy preparation process.

Inactive Publication Date: 2021-03-19
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The requirements for equipment are not high, and silicon dots with temperature-sensitive characteristics have not been reported

Method used

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  • Preparation method of polyisoacrylamide sphere modified fluorescent silicon dot, prepared fluorescent silicon dot and application of fluorescent silicon dot
  • Preparation method of polyisoacrylamide sphere modified fluorescent silicon dot, prepared fluorescent silicon dot and application of fluorescent silicon dot
  • Preparation method of polyisoacrylamide sphere modified fluorescent silicon dot, prepared fluorescent silicon dot and application of fluorescent silicon dot

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Preparation of polyiscrylamide ball modified fluorescent silicon dots

[0039] 1, preparation of silicon nanocrystalline quantum dots

[0040] 0.3180 g sodium citrate was added to 8 ml of glycerol, and argon was attached, and 2 ml of 3-aminopropyltriethoxysilane (APTES) was added after stirring for 20 min, and the mixture was transferred to the oil bath. In the pot, the temperature was raised to 185 ° C to continue to react 1.5 h. After the reaction, the cooling to room temperature was cooled to a silaneum dot crude solution to the dialysis bag having a crystal molecular weight of 3500 dA (MWCO: 3500 Da), and the ultrapure water was used as dialysate, dialyzed 24h, and collected a solution in the dialysis bag to obtain silicon nanometers. The crystalline sub-solution, protected from light.

[0041] 2. Preparation of the modified silicon nanocrystalline quantum dots in folic acid

[0042] 2 ml silicon nanocrystalline quantum dots were added to the round bottom flask, followe...

Embodiment 2

[0046] Example 1 The optical properties of the fluorescent silicon dots modified by polyishalamide balls were prepared.

[0047] The fluorescent silicon dots modified in 10 mg of Embodiment 1 were weighed, and the PBS buffer solution (0.1 mm) of a concentration of 10 mg / ml was mounted.

[0048] Fluorescence spectrum test: Test the fluorescence emission spectrum of the above solution. Fluorescence emission spectroscopy was initiated at 404 nm, and the slit width of excitation and emission was 5 nm / 5 nm. The resulting fluorescence emission spectrum is shown figure 1 Description Successfully synthesized silicon dots with strong blue fluorescence. The experimental results show that the polyisacrylamide ball modified fluorescent silicon dots still have strong fluorescence.

Embodiment 3

[0050] Example 1 The temperature response performance of the polyishalaamine-modified fluorescent silicon dots made.

[0051] The fluorescent silicon dots modified in 10 mg of Embodiment 1 were weighed, and the PBS buffer solution (0.1 mm) of a concentration of 10 mg / ml was mounted.

[0052] Fluorescence Spectroscopy: Take the above solution to measure the fluorescence emission spectrum in 17 ° C and 47 ° C, repeat 5 times. Fluorescence emission spectroscopy was initiated at 404 nm, and the slit width of excitation and emission was 5 nm / 5 nm. The resulting temperature response performance diagram figure 2 , figure 2 It is shown that the fluorescent silicon dots of the polyishalamine sphere modified as the temperature gradually decreases, and the fluorescent silicon dot has a expected temperature responsive performance. The experimental results show that the repeated thermal cycle does not affect the fluorescence intensity of the fluorescent silicon dots modified by the polyi...

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Abstract

The invention discloses a preparation method of a poly(N-isopropyl acrylamide) sphere modified fluorescent silicon dot, the prepared fluorescent silicon dot and application. The preparation method comprises the following steps: reacting sodium citrate and 3-aminopropyl triethoxysilane in glycerol to prepare a silicon nanocrystalline quantum dot; reacting silicon nanocrystalline quantum dots, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride, N-hydroxysuccinimide and folic acid in pure water to obtain folic acid modified silicon nanocrystalline quantum dots; and reacting N-isopropylacrylamide, N,N'-methylene diacrylamide, the folic acid modified silicon nanocrystalline quantum dots and potassium persulfate in pure water to obtain the fluorescent silicon dots modified by the polyisoacrylamide spheres. The polyisoacrylamide sphere modified fluorescent silicon dot prepared by the method is low in cytotoxicity, has a function of responding to temperature change, can be used for cancer cell in-situ imaging and temperature detection, and is simple and feasible in preparation process and easy for large-scale production.

Description

Technical field [0001] The present invention belongs to the field of fluorescent silicon dots, and more particularly to a method for preparing a fluorescent silicon dot of polyishalactamide and its preparation of fluorescent silicon dots and applications thereof. Background technique [0002] The intracellular temperature is the basic characteristics of cell activity, and the release of complex neurotransmitters can cause changes in intracellular temperature. In addition, cancer cell death caused by high temperatures may activate the immune system against tumors. Therefore, the detection of intracellular temperature is important in biomedicine. Poly (N-isopropyl acrylamide) (PNIPAM) as a polymer polymer, the lowest critical dissolution temperature (LCST) is about 33 ° C, and when the ambient temperature is below LCST, the PNIPAM can change the volume, and it is water. Good sexual materials, while when the ambient temperature is higher than LCST, pnipam is hydrophilic, and will sh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08F292/00C08F220/54C08F222/38C09K11/59G01N21/64G01K11/00A61K49/00B82Y20/00B82Y40/00
CPCC08F292/00C09K11/59G01N21/6486G01K11/00A61K49/0019A61K49/0054A61K49/0093B82Y20/00B82Y40/00C08F220/54C08F222/385
Inventor 杨雅琼李以婷明灯明杨欣煜张立慧石优红黄佳玲
Owner NANJING UNIV OF TECH
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