Preparation method of polyisoacrylamide sphere modified fluorescent silicon dot, prepared fluorescent silicon dot and application of fluorescent silicon dot
A technology of acrylamide balls and silicon dots, which is applied in the field of fluorescent silicon dots, achieves the effects of solving poor biocompatibility and poor selectivity of temperature-sensitive detection of cancer cells, being easy to produce on a large scale, and having a simple and easy preparation process.
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Embodiment 1
[0038] Preparation of polyiscrylamide ball modified fluorescent silicon dots
[0039] 1, preparation of silicon nanocrystalline quantum dots
[0040] 0.3180 g sodium citrate was added to 8 ml of glycerol, and argon was attached, and 2 ml of 3-aminopropyltriethoxysilane (APTES) was added after stirring for 20 min, and the mixture was transferred to the oil bath. In the pot, the temperature was raised to 185 ° C to continue to react 1.5 h. After the reaction, the cooling to room temperature was cooled to a silaneum dot crude solution to the dialysis bag having a crystal molecular weight of 3500 dA (MWCO: 3500 Da), and the ultrapure water was used as dialysate, dialyzed 24h, and collected a solution in the dialysis bag to obtain silicon nanometers. The crystalline sub-solution, protected from light.
[0041] 2. Preparation of the modified silicon nanocrystalline quantum dots in folic acid
[0042] 2 ml silicon nanocrystalline quantum dots were added to the round bottom flask, followe...
Embodiment 2
[0046] Example 1 The optical properties of the fluorescent silicon dots modified by polyishalamide balls were prepared.
[0047] The fluorescent silicon dots modified in 10 mg of Embodiment 1 were weighed, and the PBS buffer solution (0.1 mm) of a concentration of 10 mg / ml was mounted.
[0048] Fluorescence spectrum test: Test the fluorescence emission spectrum of the above solution. Fluorescence emission spectroscopy was initiated at 404 nm, and the slit width of excitation and emission was 5 nm / 5 nm. The resulting fluorescence emission spectrum is shown figure 1 Description Successfully synthesized silicon dots with strong blue fluorescence. The experimental results show that the polyisacrylamide ball modified fluorescent silicon dots still have strong fluorescence.
Embodiment 3
[0050] Example 1 The temperature response performance of the polyishalaamine-modified fluorescent silicon dots made.
[0051] The fluorescent silicon dots modified in 10 mg of Embodiment 1 were weighed, and the PBS buffer solution (0.1 mm) of a concentration of 10 mg / ml was mounted.
[0052] Fluorescence Spectroscopy: Take the above solution to measure the fluorescence emission spectrum in 17 ° C and 47 ° C, repeat 5 times. Fluorescence emission spectroscopy was initiated at 404 nm, and the slit width of excitation and emission was 5 nm / 5 nm. The resulting temperature response performance diagram figure 2 , figure 2 It is shown that the fluorescent silicon dots of the polyishalamine sphere modified as the temperature gradually decreases, and the fluorescent silicon dot has a expected temperature responsive performance. The experimental results show that the repeated thermal cycle does not affect the fluorescence intensity of the fluorescent silicon dots modified by the polyi...
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