Washing-free long-time cell membrane fluorescence imaging reagent and preparation method thereof

A fluorescence imaging, long-term technology, applied in the field of biomedicine, can solve the problem of inability to clean the fluorescent dye, and achieve the effect of no cleaning, low luminous efficiency, and good biocompatibility
CN104288787AInactive Publication Date: 2015-01-21SOUTHEAST UNIV
2 Cites 11 Cited by

Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
SOUTHEAST UNIV
Publication Date
2015-01-21
Estimated Expiration
Not applicable · inactive patent

Smart Images

  • Figure 1
    Figure 1
  • Figure 2
    Figure 2
  • Figure 3
    Figure 3
Patent Text Reader

Abstract

The invention provides a washing-free long-time cell membrane fluorescence imaging reagent. The side chain of the reagent is an ethylene glycol chitosan macromolecule containing a hydrophobic unit and a low-efficiency fluorescence unit. Based on multi-site anchoring, the reagent has the advantages of good biocompatibility, simple synthesis, low cost and convenience in staining, does not need washing, cannot be easily endocytosed by cells, and can be used as a long-time cell membrane specificity tracing labelling probe.
Need to check novelty before this filing date? Find Prior Art

Description

technical field

[0001] The invention belongs to the technical field of biomedicine, and in particular relates to a long-time cell membrane fluorescence imaging reagent based on multi-site anchoring and no-cleaning, and also relates to a preparation method of the reagent. Background technique

[0002] The cell membrane not only wraps individual cells structurally to form a stable internal environment, but also participates in material transport, energy transfer, signal transduction, vesicle transport, cell growth, differentiation, adhesion, transfer, stress, and endocytosis functionally , exocytosis, apoptosis, necrosis, autophagy and many other important biological processes, so more and more attention has been paid to the research on cell membranes. Fluorescent staining of cell membranes can be used to mark and track cell membranes, and has become a powerful method for studying the structure and function of cell membranes.

[0003] Currently commercially available lipophil...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More