23 results about "Fluorescence staining" patented technology

Disclosed in the present disclosure are a determination system having multiple detection modes and a related method. The determination system having multiple detection modes comprises: a sample stage; an optical module used for scanning a sample under test, the optical module at least having a sequencing mode and a fluorescence staining mode; a driver circuit selectively coupled to mode parameters corresponding to an operation mode of the optical module, the mode parameters at least including a first mode control parameter corresponding to the sequencing mode and a second mode control parameter corresponding to the fluorescence staining mode; and a mode switching circuit coupled to the driver circuit to control the driver circuit on the basis of the sample under test: the mode switching circuit associates different mode parameters with the driver circuit on the basis of the type of the sample under test. The present disclosure achieves multi-mode switching of the detection system, and achieves multiple detection functions on a same platform.

This invention relates to a method for rapidly screening filamentous fungi that produce high levels of triglycerides and polyunsaturated fatty acids, belonging to the field of microbial technology. The method employs a two-stage screening strategy of "resistance primary screening – fluorescence secondary screening." In the primary screening stage, strains with strong lipid synthesis capabilities are initially screened by adding a combination of phytocyanin and quizalofop-p-ethyl inhibitors to the culture medium. In the secondary screening stage, cellophane culture technology and Nile red fluorescence staining are combined to quantitatively assess the triglyceride content of the colonies, achieving rapid and non-destructive screening. This method is simple to operate, highly efficient, and shows a significant positive correlation between fluorescence intensity and triglyceride content. It is suitable for mutagenesis breeding and industrial strain selection of oil-producing filamentous fungi such as *Morchella alpineensis*.

The utility model belongs to the technical field of immunofluorescent staining, and particularly relates to an immunofluorescent staining tool kit which comprises a base, the cleaning module is detachably mounted on the base, and the cleaning module is used for cleaning the used cover glass; the dyeing module is detachably mounted on the cleaning module, and the dyeing module is used for dyeing the cover glass; and the cover plate is detachably mounted on the dyeing module. The utility model is convenient to use and can ensure the dyeing effect. The evaporation of the reagent in the long-time incubation process can be reduced, so that the dyeing effect is more stable. In addition, independent dyeing during simultaneous incubation and transfer of a plurality of cover glass can be guaranteed, and mutual confusion can be avoided. The cleaning module can simultaneously meet different requirements of rapid cleaning and long-time cleaning of the cover glass, reagent adding can be completed at a time, and the cumbersome step of replacing reagents for multiple times is omitted. Meanwhile, due to the detachable design, all the components can be cleaned and dried more easily, and the cover glass can be taken and placed conveniently, and cleaning liquid can be replaced conveniently.

The invention provides a multiplex fluorescence immunohistochemical detection kit for rare gastrointestinal tumors and application of the multiplex fluorescence immunohistochemical detection kit, and belongs to the technical field of multiplex immunohistochemistry. The invention provides a multiplex fluorescence immunohistochemical detection reagent combination, which comprises a monoclonal antibody designed for protein markers and a fluorescent dye, and can be applied to multiplex fluorescence immunohistochemical detection for detecting the protein markers such as PD-L1, PD-1, CTLA-4, LAG-3, TIM-3 and TIGIT. The invention further provides a kit capable of being used for multiplex fluorescence immunohistochemical detection, staining detection of six protein markers on a single section can be achieved, and multiplex fluorescence staining of disease samples, especially complex-structure gastrointestinal tract rare tumor samples is achieved by matching six protein targets according to a specific staining sequence and fluorescence channels. And false positive caused by staining crosstalk of each channel is prevented.

The invention belongs to the technical field of biomedical detection, and discloses a blood cell analysis system based on image flow cytometry, which comprises a cell staining module, the dyeing module simultaneously comprises a first fluorescent probe capable of being specifically combined with components in a cell nucleus or enriched in the cell nucleus, a second fluorescent probe capable of being specifically combined with cell nucleus DNA and a third fluorescent probe capable of being specifically combined with a blood cell differentiation antigen; an image flow cytometer; and the image processing module is used for calculating the kernel detection rate and / or the average kernel number. According to the present invention, the combination of the specific types of the cell staining dyes is adopted, the image flow cytometry is matched, and the optimized multi-dimensional fluorescence staining combination and the matched automatic image analysis process are adopted to achieve the synchronous, high-throughput and accurate quantitative analysis of the blood cell immune phenotype and the cell morphology key characteristics at the single cell level;

The blood detection method provided by the embodiments of the present disclosure comprises: performing multiple-label fluorescence staining on a blood sample to be detected; collecting a fluorescence signal of the multiple labels by a fluorescence microscope; processing the fluorescence signal based on a signal separation algorithm to reduce background noise; and analyzing the processed fluorescence signal to obtain a detection result. The embodiments of the present disclosure can solve the problem of background noise caused by signal cross interference in multiple-label fluorescence detection.

The invention discloses a multiplex fluorescence immunohistochemical detection kit for rare lymphoma and application of the multiplex fluorescence immunohistochemical detection kit. The multiplex fluorescence immunohistochemical detection kit for rare lymphoma comprises a primary antibody reagent and a fluorescence staining reagent, the primary antibody reagent is prepared from a rabbit anti-human CD15 monoclonal antibody, a rabbit anti-human CD30 monoclonal antibody, a KI-67 monoclonal antibody, a rabbit anti-human CD79b monoclonal antibody, a rabbit anti-human CD3 monoclonal antibody and a rabbit anti-human CD20 monoclonal antibody. When the multiplex fluorescence immunohistochemical detection kit for the rare lymphoma is used, a false positive result caused by channel spectrum crosstalk is avoided to the greatest extent through a specific staining sequence of each target protein and fluorescein matching, and the purpose of detecting the rare lymphoma, especially the rare type of lymphoma, is achieved. The kit can be used for synchronous detection of lymphoma, such as hodgkin lymphoma, mantle cell lymphoma and marginal region lymphoma, and provides a reliable basis for lymphoma immune targeted therapy.

The invention belongs to the technical field of aquatic pathogenic microorganism products, and particularly relates to an in-vitro culture method of enterospora hepatopenaei. The method comprises the following steps: obtaining purified spores of enterospora hepatopenaei; the method comprises the following steps: inoculating purified spores of enterospora hepatopenaei into a host cell being cultured for infection to obtain a host cell sample infected with enterospora hepatopenaei; and detecting the proliferation and maturation of the purified spores of the enterospora hepatopenaei in the host cell sample infected with the enterospora hepatopenaei by using a fluorescent staining method. The method provided by the invention provides a key technical platform for basic research, pathogenic mechanism analysis, drug screening and vaccine development of the shrimp enterospora hepatopenaei, has the advantages of convenience in acquisition of experimental materials, simplicity and convenience in operation, high repeatability and the like, and is suitable for in-vitro culture and proliferation of the pathogen in a laboratory.

The invention relates to the technical field of deep learning, and discloses a prediction method and system for a liver cancer immunotherapy effect. The method comprises the following steps: carrying out multiple immunofluorescence staining pretreatment on a clinical sample, and collecting forward scattering data, lateral scattering data and multi-channel fluorescence data of mononuclear cells to obtain a cell expression initial data matrix; performing standardization processing to obtain a multi-dimensional feature matrix expressed by the immune factors; performing Bayesian network analysis, calculating a conditional probability relation and a mutual information value between immune cell factors, and constructing an immune factor association network; performing anomaly detection to obtain an immune factor expression anomaly detection result; and performing minimum tail boundary analysis on the immune factor expression anomaly detection result, solving an optimal risk boundary through a semi-definite programming model, and outputting immune factor anomaly quantitative evaluation data, thereby realizing quantitative risk evaluation of the prediction result and refined grading of the prediction result.

The invention is applicable to the technical field of biological detection, and provides a specific antibody and application thereof in detection and enrichment of oocysts of cryptosporidium. According to the method, an immunomagnetic separation detection system is constructed by taking oocyst outer wall protein (cgd6660) as a target: an antibody capable of being specifically combined with the protein is subjected to purification and biotinylation and then is coupled to the surface of a streptavidin magnetic bead, so that efficient and specific capture of the oocyst is realized. The system is efficient and specific in combination with oocysts of cryptosporidium, interference of impurities such as algae and bacteria can be effectively avoided, and the system has good applicability and stability in complex water quality samples with high turbidity, high organic matter and the like. The method is high in process standardization degree and short in operation time, and the autonomously prepared local antibody greatly reduces import dependence and detection cost; by combining fluorescent staining and microscopic confirmation, sensitive and accurate detection of oocysts can be realized, the method is suitable for scenes such as drinking water source monitoring and the like, and stable and reliable technical support is provided for water safety and cryptosporidiosis prevention and control.

The present application relates to a kind of ADTKD-UMOD based on deep learning auxiliary diagnosis system, belong to auxiliary diagnosis technical field, solve the problem that prior art lacks ADTKD-UMOD auxiliary diagnosis.System includes: data acquisition module, collects the staining image of renal tubular pathological section and multi-channel fluorescence staining image;Contour drawing module, based on the multi-channel fluorescence staining image of each individual renal tubular pathological section obtains the medullary loop ascending branch thick segment renal tubular contour in staining image;Training sample construction module, based on the staining graph that has outlined contour, constructs training sample set;Model training module, constructs deep learning network model, based on training sample set, carries out semi-supervised training to deep learning network model and obtains prediction model;Prediction module, the renal tubular contour map in the staining image of individual to be predicted is inputed into prediction model and obtains prediction result.Quick ADTKD-UMOD prediction is realized.

The invention relates to the technical field of immunofluorescent staining, in particular to an adipose tissue whole-tissue immunofluorescent staining method based on tabletting treatment. According to the method, tissue acquisition and fixation, tissue tabletting, membrane rupture and sealing, degreasing and autofluorescence removal, fluorescent staining, sheet sealing and imaging are sequentially carried out. According to the invention, a complete tabletting-degreasing-fluorescent staining integrated process is established, a set of systematic process which is standardized, high in repeatability and adaptive to conventional experimental conditions is provided, and tabletting whole tissue staining is realized in adipose tissues for the first time. The core of the invention lies in that the thickness of the tissue is reduced by a tabletting treatment means, and a set of optimized antibody permeation, sealing, degreasing and anti-autofluorescence treatment flow is matched, so that the immunofluorescent staining of the adipose tissue is effectively realized on the premise of not depending on complex tissue transparency and special imaging equipment, and the immunofluorescent staining effect is greatly improved. The method is especially suitable for detecting deep or low-abundance targets such as nerve markers.

The embodiments of the present application provide a PWM dimming device, a fluorescence microscopy optical system, and a fluorescence-stained cell scanning and analysis system. The PWM dimming device includes: a voltage source; a PWM controller for controlling the on / off of the voltage source to output a pulsed voltage; an LED light source device to which the pulsed voltage is applied. The fluorescence microscopy optical system includes the above-mentioned PWM dimming device. The fluorescence-stained cell scanning and analysis system includes the above-mentioned PWM dimming device. The fluorescence-stained cell scanning and analysis system includes a fluorescence microscopy optical system. The embodiments of the present application solve the technical problems of low adjustment frequency and low adjustment accuracy of the traditional dimming device of the fluorescence-stained cell scanning and analysis system.

The present invention discloses a non-washing two-color cell imaging fluorescein carbon dot staining solution for fungal detection, which, by mass percentage, comprises: 0.50% - 1.50% of fluorescein carbon dot material and 98.50% - 99.50% of water; wherein the fluorescein carbon dot material is made from raw materials with the following mass percentages: 0.5% - 1.3% of fluorescent brightener 135#, 25% - 29% of ethylenediamine, 0.05% - 0.13% of ammonium persulfate, and 69.57% - 74.45% of water. Also disclosed is a method for fungal staining using the above staining solution and the application of the above fluorescein carbon dot staining solution in fungal detection using fluorescence staining method. In particular, this staining solution can accurately determine the presence of epidermal fungi without removing dandruff, has simple components, can remain stable for a long time, and greatly reduces the cost.

The application belongs to the field of neurology and provides a method for detecting neuron death by coupling fluorescence and electron microscopy, comprising the following steps: step one, using Pulsineli's four-vessel occlusion method to make a rat transient global ischemia model, and sampling and processing the model animal to ensure the stability of the sample during the experiment; step two, performing monochromatic fluorescence staining on the sample obtained in step one, then converting the Rhodamine R6 fluorescent label in the slice into 3,3'-diaminobenzidine precipitation, and making the slice into an electron microscopy sample, and determining the experimental results. The application first applies Rhodamine R6 to the field of ultra-morphological detection of neuron death, converts the fluorescent signal of Rhodamine R6 into diaminobenzidine precipitation, thereby realizing the coupling of fluorescence and electron microscopy, and observing the ultra-localization of the positive substance in the dead cells by using an electron microscope.

The invention relates to cell biology and regenerative medicine, specifically to a culture method and application for porcine limbal epithelial stem cells. The method involves pretreatment of cells, coating of culture dishes, centrifugation, culturing, passaging, and identification by immunofluorescence staining. The cultured cells are applicable in repairing corneal diseases, including limbal stem cell deficiency. During repair, the cells can be combined with biological materials to form a stem cell engineering carrier for implantation at the injury site. The separation and culture method is simple, yields a large number of cells, and is suitable for use with porcine eyeballs. Limbal epithelial stem cells cultured in vitro form distinct cell clusters, referred to as stem cell islands. The obtained cells exhibit phenotypes including BMI1+, ΔNp63α+, CK14+, and ABCG2+. Based on experimental and clinical research, this method provides a promising approach for cell transplantation in ocular surface reconstruction.

The invention belongs to the technical field of biology, and discloses a lycoris radiata meiosis cell protein immunofluorescence staining method, which comprises the following steps: 1) treating a fixed anther to obtain a pollen mother cell in a meiosis period; 2) performing enzyme treatment to decompose cytoplasm of the meiotic cells; (3) carrying out slide preparation and fluorescent staining by using the treated meiotic cells; and 4) observing the positioning signal of the target protein under a fluorescence microscope. The pollen mother cells are treated by using glucanase and the like, so that a thicker callose protective layer on the outer layer of the lycoris radiate pollen mother cells is effectively decomposed, and the binding of an antibody and chromosomes is facilitated; meanwhile, a film preparation method is improved, and expression of fluorescence signals is effectively enhanced. The method is of great significance in researching subcellular localization of proteins with different functions in pollen mother cells of lycoris radiate in a meiosis period by using an immunofluorescence localization technology.

The application discloses a rapid iteration type TSA multiple immunofluorescence staining kit, relates to the technical field of multiple immunofluorescence staining, and comprises an operation table, an elution tank is installed on the outer wall of the operation table, a shaking-off clamp is installed on the inner wall left side of the elution tank, the shaking-off clamp is connected with the elution tank through a rotating shaft one, a dropwise adding device is installed on the outer wall rear side of the elution tank, the dropwise adding device is connected with the operation table through a moving assembly, a liquid storage tank one is installed on the inner wall rear side of the operation table, a pressure pump one is installed on the outer wall upper side of the liquid storage tank one, and the dropwise adding device is connected with the pressure pump one through a connecting pipe. Through the installation of the shaking-off clamp, the dropwise adding device, the moving assembly and the rotating shaft two, the function of precise dropwise adding of eluent is realized, the problems of fixed eluent dropwise adding parameters, slice tissue damage, uneven eluent coverage and cross contamination are solved, fragile samples can be protected, and the consistency of elution effect is improved.

Disclosed in the present invention is a compound dye for cell fluorescence staining in a complex matrix. The formula of the compound dye, based on a total volume of 100 mL, comprises: a buffer system with a pH of 6.0-8.0; a fluorescent dye which is Sybr Gold at a concentration of 2× to 40× or 2-80 mg of propidium iodide; 0.1-10 mL of triton X-100; 0-24 mL of glycerol; 0-800 mg of EDTA-disodium; and a polyamidoamine dendrimer. The two preferred compound fluorescent dye formulas of kits of the present invention can both achieve efficient staining of cells in a complex matrix and accurate counting of the cells, and the dye has excellent fluorescent visualization capability. Compared with other dyes, the compound dye has a higher staining rate (staining can be performed within 30 seconds) and a stable staining effect, and is not prone to fluorescence quenching under long-term illumination.

This invention discloses the pear transcription factor PbrMYB24 and its application in promoting lignin and cellulose synthesis in pear stone cells. The PbrMYB24 gene, isolated from 'Dangshan Crisp Pear', has the nucleotide sequence shown in SEQ ID No. 1. Transient overexpression in pear fruit confirmed that PbrMYB24 can promote the accumulation of lignin and cellulose in fruit stone cells. In transgenic pear pulp callus tissue and Arabidopsis inflorescence stems overexpressing PbrMYB24, lignin and cellulose contents were significantly increased, and the signals of phloroglucinol hydrochloride staining, Fast Green staining, and Karl von Willebrand fluorescence staining were significantly enhanced at the same time. This demonstrates that the PbrMYB24 gene participates in regulating the formation of lignin and cellulose in pear fruit stone cells.

A super-resolution microscopy imaging system based on mask phase modulation is sequentially arranged as follows: an illumination source for generating monochromatic plane waves; an optical mask for modulating the phase of each adjacent region; an imaging sample for reflecting the generated monochromatic plane waves; a microscope objective and a tube lens for receiving far-field light intensity; and a CCD for imaging. Unlike ordinary optical microscopes, this invention adds an optical mask that can improve the system's imaging resolution, enabling the system to overcome the diffraction limit and obtain super-resolution imaging effects. Moreover, the imaging resolution depends only on the unit size of the mask. Compared with other super-resolution imaging technologies, this invention does not require point-by-point scanning imaging, fluorescence staining, or image post-processing. It also has strong compatibility with ordinary optical microscopes and features simple operation, cost savings, and high efficiency.

The application relates to the technical field of deep learning, and discloses a liver cancer immunotherapy effect prediction method and system.The method comprises the following steps: performing multiple immunofluorescence staining pretreatment on a clinical sample, collecting forward scattering data, side scattering data and multi-channel fluorescence data of single nucleus cells, obtaining a cell expression initial data matrix, performing standardization processing to obtain a multi-dimensional feature matrix of immune factor expression, performing Bayesian network analysis to calculate the conditional probability relationship and mutual information value between immune cell factors, constructing an immune factor correlation network, performing abnormality detection to obtain immune factor expression abnormality detection results, performing minimum tail boundary analysis on the immune factor expression abnormality detection results, solving an optimal risk boundary through a semi-definite programming model, and outputting immune factor abnormality quantitative evaluation data, so that quantitative risk evaluation of a prediction result and fine classification of the prediction result are realized.