95 results about "Fluorescence staining" patented technology

The invention discloses a modified cellulose nanofiber membrane based on layer-by-layer self-assembly of lysozyme and silk protein as well as preparation and application thereof and belongs to the technical fields of high polymer materials and biomedical materials. According to the invention, a cellulose acetate nanofiber membrane is prepared by utilizing an electrospinning technique and lysozyme and silk protein which are opposite in charges are alternately assembled on the surface of the cellulose nanofiber membrane by adopting a layer-by-layer assembly technique so as to obtain the modified cellulose nanofiber membrane disclosed by the invention. The membrane disclosed by the invention has the advantages that the preparation equipment is simple, raw materials are low in cost, non-toxic and biodegradable, the whole process is simple to operate and the number of lysozyme / silk protein layers is controllable. Proved by antibacterial tests, cell survival tests, cell fluorescence staining tests, cell adhesion tests and in vitro wound healing tests, the modified cellulose nanofiber membrane disclosed by the invention has good antibacterial performance and cell adhesiveness and is capable of increasing the wound healing efficiency and preventing the wound infection, so that the membrane is a very good wound repairing material and can be used in the wound repairing field.

The invention discloses a preparation method of a sample for conveniently and rapidly detecting deoxyribonucleic acid (DNA) cell damage. The method mainly comprises a gel sheet-making step, a cell lysis step, a DNA melting step, an electrophoresis step, a neutralizing step and a dyeing step, wherein in the gel sheet-making step, two layers of gels need to be spread; and in the dyeing step, a GelRed nucleic acid gel dye with high sensitivity and low toxicity is used. The invention also provides a kit using the method. The kit comprises normal melting-point agarose, low melting-point agarose, a cell lysis solution, an electrophoretic buffer solution, the DNA gel dye and a frosted edgeglass slide. According to the method, fluorescence dyeing is carried out by using the GelRed nucleic acid gel dye, and the GelRed nucleic acid gel dye is high in sensitivity, low in toxicity and stable, and environment pollution cannot be caused by wastes, so that the method is safe and environment-friendly; and because of only two layers of the spread gels, compared with a sandwich gel-spreading method used in the traditional comet assay, the preparation method is easy to operate and difficult to degum, and is uniform in dyeing; and moreover, the obtained electrophoresis image is relatively clear and objective.

A standard material that is used for judging an abnormal portion in a particle analyzer is described. The standard material comprises first standard particles to be fluorescence-stained by a fluorescence-staining treatment and second standard particles that have preliminarily contained a fluorescence dye.A method and an analyzer that can judge an abnormal portion in a particle analyzer by using such a standard material are also described.

The invention discloses a floating sphere immunofluorescent staining method and a staining device, belonging to methods for coloring a sample for test. The staining method comprises the following steps: separation of spheres from a culture medium, fixing by stationary liquid, adding of Triton X-100 transparent cells, closing by confining liquid, adding of an interest protein-resisting primary antibody working solution, adding of a primary antibody-resisting fluorescent secondary antibody working solution, and cell nucleus staining by DAPI dye liquor. The staining device comprises a cell chamber and a staining sleeve part arranged at the outer part of the lower end of the cell chamber in a sleeving way. The floating sphere immunofluorescent staining method has the advantages of high efficiency, simpleness, convenience and the like, an antibody usage amount is reduced, experiment cost is reduced, manpower and time are saved, an accurate and reliable experiment result is obtained on the basis that a fundamental principle that immunofluorescent staining is not changed and specially training experimenters is not needed, sphere loss in an experiment operation process is avoided, an experiment success rate is increased, and the efficiency is improved.

The invention discloses a sperm fluorescence staining method, which comprises the steps of: mixing sperms with PI, Transgreen and Transgreen / PI, and putting the mixture into an incubator with 5 percent CO2 and temperature of 37 DEG C for cultivation. Applying a computer to assist a sperm and semen quality analysis system and analyze changes of all movement parameters of sperms after adding fluorescent dye and Transgreen / PI redyeing is feasible for calculating the survival rate of sperms.

The invention relates to an image processing system for generating a simulated digital bright field IHC or ISH image from monochromatic, fluorescence images (106.1-106.7) of a tissue sample (100). Thetissue sample comprises one or more stained biomarkers, each of the stained biomarkers being stained by a respective fluorescence stain, the system being configured for: receiving (702) a first one of the fluorescence images generically indicating the presence of biological matter; transforming (704) the first image into a transformed first image having a first color; for each of the biomarkers,receiving (706) a respective second one of the fluorescence images indicating signals emitted by the fluorescence stain selectively staining said biomarker, transforming (708) each of the second images into a respective transformed second image having a respective second color; overlaying and combining (710) the transformed first and one or more second images; storing (712) and / or displaying (714)the combined image as the simulated digital bright field IHC or ISH image, wherein the first image is crated using an autofluorescence reference spectrum of the tissue sample or of a similar tissue sample or by using a fluorescence reference spectrum of a first stain which generically binds to biological matter of the tissue sample for spectrally unmixing of a multi-spectral digital image of thetissue sample.

The invention discloses thiazole orange cyanine dye molecules and application thereof. The dye molecules structurally comprise binding groups, ELISA groups, crosslinking groups and connecting parts. The molecules are applied to cell fluorescence staining and are used for judging the living states of bacteria based on the bacterium esterase activity and used for selectively inhibiting the amplification of bacterium DNA in PCR reaction, so that that the purpose of rapidly and quantitatively detecting living bacteria is achieved. The dye molecules disclosed by the invention are simple in structure, low in preparation cost and high in esterase degradation rate.

The invention discloses a method for observing the deposition of callose of Agrostis stolonifera blade tissues based on a paraffin slice and an aniline blue fluorescence staining technology. The method comprises the following steps: 1, preparing a medicine; 2, making the paraffin slice sequentially through the steps of material drawing, fixation, dehydration, transparentizing, paraffin impregnation, embedding, slicing, slice flatting, slice drying, staining and slice sealing, staining the obtained slice by using a fluorescence staining agent, and observing and shooting the stained slice under a fluorescence microscope. The method for observing the deposition of callose of Agrostis stolonifera blade tissues based on the paraffin slice and the aniline blue fluorescence staining technology greatly shortens the experiment time of Agrostis stolonifera paraffin slice production, overcomes the difficulty in the production of the small and fine slice of an Agrostis stolonifera blade, breaks restriction brought by the material and environment factors, rapidly and highly-efficiently completes the paraffin slice production process, and finally obtains an excellent callose fluorescence microscopic observation result.

A fungus fluorescence staining liquid is disclosed and comprises an A staining liquid and a B staining liquid. The A staining liquid includes 0.03-0.1 part by weight of fluorescein, 1-5 parts by weight of a cleaning agent and 80-150 parts by weight of water. The B staining liquid includes 0.3-1 part by weight of dye and 80-150 parts by weight of water. The A staining liquid can combine cell wall beta-polysaccharides of various funguses, thus labeling and fluorescing for detection. The B staining liquid is adopted as a counterstaining liquid and used for reducing the fluorescence background. The fungus fluorescence staining liquid comprising the A staining liquid and the B staining liquid is a rapid fungal infection detection product and can combine various funguses to emit fluorescence, thus detecting whether various suspected fungal infection diseases are fungal infection or not.

The invention provides a reagent kit for detecting colorectal cancer on the basis of liquid biopsy. The reagent kit comprises staining enhancement solution for enhancing staining effects and specific antibodies with fluorescence staining markers. The specific antibodies include CK20 antibodies, CD45 antibodies and CDX2 antibodies; the staining enhancement solution comprises surfactants with the concentration of 0.001-1 mg / mL. The reagent kit has the advantages that target cells can be effectively enriched, and whether the enriched target cells come from early-stage patients who suffer from the colorectal cancer or not can be confirmed; the tumor detection sensitivity can be improved by means of double-tumor-marker detection, and the detection accuracy further can be guaranteed by means of CEP8 detection; the staining effects can be enhanced by the staining enhancement solution, accordingly, the diversified antibodies with the fluorescence staining markers can be combined with the target cells, the target cells can be stained, the good staining effects can be realized, fluorescence is intensive, and boundaries are clear.

Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.

Single dye fluorescent staining and the combination of differences in both intensity and spectral emission permit determination of the minimum concentration of an antibiotic needed to inactivate bacteria (Minimum Inhibitory Concentration (MIC)), thereby providing a means for rapid Antibiotic Susceptibility Testing (AST). This allows for a quick and easy means for clinicians to determine a suitable treatment regimen for patients suffering from bacterial infections and those that eventually lead to sepsis.

The invention discloses a full-automatic mycobacterium tuberculosis microscanning analyzer which comprises a rack, a slide conveying device, a slide rack, a scanning device, a nozzle clamping mechanism, an optical instrument, a computer and an oil injection system; according to the invention, the slide conveying device is adopted to realize extraction and storage of slide samples between the sliderack and the objective table; a scanning device is adopted to accurately send the sample to a detection position specified by an optical instrument, and microscopic scanning is carried out; a computer and a PLC (Programmable Logic Controller) are used for controlling a first three-dimensional driving power head, a second three-dimensional driving power head and a clamping nozzle driving head to operate and controlling switching of a fluorescence switcher on a fluorescence-Candida mechanism, realizing scanning of Ziehl-Neelsen acid-fast staining and fluorescence staining slides, and operational analysis of microscopic images is performed by adopting a computer and a CPU (Central Processing Unit) to finish full-automatic microscopic scanning work of a detected slide sample. The full-automatic mycobacterium tuberculosis microscanning analyzer has the advantages of high automation degree, large slide rack capacity and high image scanning precision.

The invention discloses application of 2-bromopalmitate (2-BP) in preparation of drugs for prevention and treatment of bone loss related diseases, and belongs to the technical field of medicine. The invention, for the first time, clearly defines that 2-BP can inhibit osteoclast differentiation process of BMMs stimulated by RANKL through various experimental techniques, including tartrate resistantacid phosphatase staining, phalloidin fluorescence staining, bone lamella bone resorption function evaluation, reverse transcription real-time fluorescence quantitative PCR, Western blot, etc. In addition, the invention clearly states that 2-BP also has effects in inhibiting osteoclast differentiation and activation in vivo, and 2-BP can significantly alleviate the pathological process of massivebone loss caused by estrogen deficiency through the establishment of a mouse model of ovariectomy-induced osteoporosis, thus providing experimental data basis in vivo for the role of 2-BP in the prevention and treatment of bone loss related diseases.