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96 results about "Fluorescence staining" patented technology
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Definition of Fluorescein Staining. Fluorescein Staining is a simple procedure for diagnosing corneal injury or foreign objects in the eye. The ophthalmologist places a strip of paper containing fluorescein at the edge of the eye. The dye rapidly leaches into the tears.
Blood analyzer, blood analysis method, and computer program product
ActiveCN102768179AScattering properties measurementsIndividual particle analysisBlood specimenHemolytic Agents
A blood analyzer, a blood analysis method, and a computer program product that can distinguishably detect abnormal lymphocytes, blasts, and atypical lymphocytes are provided. A blood analyzer prepares a first measurement sample from a first reagent containing a hemolyzing agent, a second reagent containing a fluorescence staining dye, and the blood specimen, and prepares a second measurement sample from a third reagent containing a hemolyzing agent, a fourth reagent containing a fluorescence staining dye, and the blood specimen. The blood analyzer measures each of the measurement samples, and distinguishably detects abnormal lymphocytes, blasts, and atypical lymphocytes in a blood specimen based on the measurement data.
Owner:SYSMEX CORP
Detection method for tree-like aging defect of cable insulation material
InactiveCN103115926AEasy diagnosisThe detection method is simple and fastOptically investigating flaws/contaminationMicroscopic imageEngineering
The invention relates to a detection method for a tree-like aging defect of a cable insulation material, belonging to the technical field of electrical equipment insulation diagnosis. The detection method comprises the steps of preparing a cable insulation material to be detected into rectangular samples to be tested, arranging a semiconductive silicon rubber and a pin electrode, and applying high pressure on a tip of the pin electrode; forming a defect channel in the samples to be tested, eliminating the high pressure applied to the pin electrode, and removing the semiconductive silicon rubber and the pin electrode from the sample to be tested; injecting a liquid with fluorescence staining function into the sample to be tested along the detect channel of the sample to be tested; and slicing the sample, scanning with a super-resolution fluorescence microscope, processing an obtained optical microscopic image and a fluorescence microscopic image, and obtaining a feature image of the tree-like aging defect of the cable insulation material to be tested. The method is simple and fast, has high experimental efficiency, is beneficial to diagnosis of the cable insulation defects from the point of view of form, and provides a key image basis for theoretical research of electric tree-like aging.
Owner:TSINGHUA UNIV
An activated luminescence material and a method for preparing the same
ActiveCN104673275AGood biocompatibilityHigh fluorescence quantum yieldBiological testingFluorescence/phosphorescenceQuantum yieldBiocompatibility Testing
The invention relates to the technical field of fluorescent material, specifically to an activated luminescence material and a method for preparing the same. Different biomolecules are combined to tetraphenyl ethylene through condensation reaction, so the biocompatible activated luminescence material having AIE / AEE features can be obtained. The biomolecules contain water soluble activated luminescence molecules with high fluorescent quantum yield, which have excellent biocompatibility in a solution state and a solid state, and crystalline polymer which can form nanosheets in a water-soluble medium. The obtained activated luminescence material can be applied to living cell fluorescence staining, sensitive explosive detecting and the like.
Owner:HKUST SHENZHEN RES INST +1
Modified cellulose nanofiber membrane based on layer-by-layer self-assembly of lysozyme and silk protein based as well as preparation and application thereof
ActiveCN103536958AExcellent adhesionAvoid infectionAbsorbent padsBandagesCell adhesionCellulose acetate
The invention discloses a modified cellulose nanofiber membrane based on layer-by-layer self-assembly of lysozyme and silk protein as well as preparation and application thereof and belongs to the technical fields of high polymer materials and biomedical materials. According to the invention, a cellulose acetate nanofiber membrane is prepared by utilizing an electrospinning technique and lysozyme and silk protein which are opposite in charges are alternately assembled on the surface of the cellulose nanofiber membrane by adopting a layer-by-layer assembly technique so as to obtain the modified cellulose nanofiber membrane disclosed by the invention. The membrane disclosed by the invention has the advantages that the preparation equipment is simple, raw materials are low in cost, non-toxic and biodegradable, the whole process is simple to operate and the number of lysozyme / silk protein layers is controllable. Proved by antibacterial tests, cell survival tests, cell fluorescence staining tests, cell adhesion tests and in vitro wound healing tests, the modified cellulose nanofiber membrane disclosed by the invention has good antibacterial performance and cell adhesiveness and is capable of increasing the wound healing efficiency and preventing the wound infection, so that the membrane is a very good wound repairing material and can be used in the wound repairing field.
Owner:HUBEI SAILUO BIOLOGICAL MATERIAL CO LTD
Nucleated Red Blood Cell Analysis System and Method
ActiveUS20120282599A1Eliminate distractionsBioreactor/fermenter combinationsBiological substance pretreatmentsRed blood cellFluorescence
Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.
Owner:ABBOTT LAB INC
Blood analyzer, blood analysis method, and computer program product
ActiveUS20120282601A1Bioreactor/fermenter combinationsBiological substance pretreatmentsBlood specimenFluorescence
A blood analyzer, a blood analysis method, and a computer program product that can distinguishably detect abnormal lymphocytes, blasts, and atypical lymphocytes are provided. A blood analyzer prepares a first measurement sample from a first reagent containing a hemolyzing agent, a second reagent containing a fluorescence staining dye, and the blood specimen, and prepares a second measurement sample from a third reagent containing a hemolyzing agent, a fourth reagent containing a fluorescence staining dye, and the blood specimen. The blood analyzer measures each of the measurement samples, and distinguishably detects abnormal lymphocytes, blasts, and atypical lymphocytes in a blood specimen based on the measurement data.
Owner:SYSMEX CORP
Preparation method of sample for conveniently and rapidly detecting deoxyribonucleic acid (DNA) cell damage and kit using same
InactiveCN103276048ANot easy to degummingEasy to operateMicrobiological testing/measurementFluorescenceA-DNA
The invention discloses a preparation method of a sample for conveniently and rapidly detecting deoxyribonucleic acid (DNA) cell damage. The method mainly comprises a gel sheet-making step, a cell lysis step, a DNA melting step, an electrophoresis step, a neutralizing step and a dyeing step, wherein in the gel sheet-making step, two layers of gels need to be spread; and in the dyeing step, a GelRed nucleic acid gel dye with high sensitivity and low toxicity is used. The invention also provides a kit using the method. The kit comprises normal melting-point agarose, low melting-point agarose, a cell lysis solution, an electrophoretic buffer solution, the DNA gel dye and a frosted edgeglass slide. According to the method, fluorescence dyeing is carried out by using the GelRed nucleic acid gel dye, and the GelRed nucleic acid gel dye is high in sensitivity, low in toxicity and stable, and environment pollution cannot be caused by wastes, so that the method is safe and environment-friendly; and because of only two layers of the spread gels, compared with a sandwich gel-spreading method used in the traditional comet assay, the preparation method is easy to operate and difficult to degum, and is uniform in dyeing; and moreover, the obtained electrophoresis image is relatively clear and objective.
Owner:JIANGSU UNIV
Method for inducing human amniotic mesenchymal stem cells to differentiate into neuron-like cells
InactiveCN103013917ANervous system cellsSkeletal/connective tissue cellsGerm layerGlial fibrillary acidic protein
The invention provides a method for inducing differentiating human amniotic mesenchymal stem cells (hAMSCs) to differentiate into neuron-like cells by adopting all-trans retinoic acids, a basic fibroblast growth factor (bFGF) and an epidermal growth factor (EGF). The method comprises the following steps of: separating the hAMSCs, carrying out primary culture of the hAMSCs, subculturing and amplifying the hAMSCs, detecting hAMSCs immunophenotyping, inducing the hAMSCs to differentiate into the neuron-like cells and carrying out cellular immunity fluorescence staining. According to the inducing method provided by the invention, umbilical cord mesenchymal stem cells are induced to differentiate into neutral stem cells by using the all-trans retinoic acids in combination of the bFGF and the EGF; the neural stem cells not only have the typical morphology of nerve cells, but also express neuron marker antigen neuron-specific emolase and astrocyte marker antigen glial fibrillary acidic proteins; and the capability of a mesenchymal cell trans-germinal layer differentiating into non mesenchymal cells is realized so that the mesenchymal cells are likely to turn into more ideal seed cells for clinical application in further.
Owner:陆华
Standard material for particle analyzer
ActiveUS7601539B2Photometry using reference valueRadiation pyrometryFluorescenceFluorescence staining
Owner:SYSMEX CORP
Floating sphere immunofluorescent staining method and staining device
The invention discloses a floating sphere immunofluorescent staining method and a staining device, belonging to methods for coloring a sample for test. The staining method comprises the following steps: separation of spheres from a culture medium, fixing by stationary liquid, adding of Triton X-100 transparent cells, closing by confining liquid, adding of an interest protein-resisting primary antibody working solution, adding of a primary antibody-resisting fluorescent secondary antibody working solution, and cell nucleus staining by DAPI dye liquor. The staining device comprises a cell chamber and a staining sleeve part arranged at the outer part of the lower end of the cell chamber in a sleeving way. The floating sphere immunofluorescent staining method has the advantages of high efficiency, simpleness, convenience and the like, an antibody usage amount is reduced, experiment cost is reduced, manpower and time are saved, an accurate and reliable experiment result is obtained on the basis that a fundamental principle that immunofluorescent staining is not changed and specially training experimenters is not needed, sphere loss in an experiment operation process is avoided, an experiment success rate is increased, and the efficiency is improved.
Owner:GENERAL HOSPITAL OF TIANJIN MEDICAL UNIV
Dual-fluorescence staining solution for vaginal microorganism detection and application thereof
ActiveCN110940646AAvoid interferenceEasy to operatePreparing sample for investigationFluorescence/phosphorescencePathogenic microorganismStaining
The invention discloses a dual-fluorescence staining solution for vaginal microorganism detection. The dual-fluorescence staining solution for the vaginal microorganism detection comprises an independent dual-fluorescence staining solution A and an independent dual-fluorescence staining solution B, wherein the dual-fluorescence staining solution A is composed of a fluorescence staining agent, an auxiliary staining agent, a staining solution buffer reagent, a bacteriostatic agent and water, and the dual-fluorescence staining solution B is composed of a staining solution buffer reagent, an alkaline regulating reagent, an anti-quenching agent, a bacteriostatic agent and water. The invention has the advantages of being simple in operation process, rapid in detection and easy to observe and recognize after pathogenic microorganism dyeing. Moreover, the interference of contaminated specimens such as bloody specimens, seminal fluid specimens and basal cell specimens on detection results is overcome, and the accuracy and the detection rate are effectively improved.
Owner:江苏美克医学技术有限公司
Sperm fluorescent dyeing determination method
InactiveCN101308131ACan dynamically evaluate the survival rateSimple methodBiological testingFluorescenceComputer-aided
The invention discloses a sperm fluorescence staining method, which comprises the steps of: mixing sperms with PI, Transgreen and Transgreen / PI, and putting the mixture into an incubator with 5 percent CO2 and temperature of 37 DEG C for cultivation. Applying a computer to assist a sperm and semen quality analysis system and analyze changes of all movement parameters of sperms after adding fluorescent dye and Transgreen / PI redyeing is feasible for calculating the survival rate of sperms.
Owner:SHANGHAI INST OF PLANNED PARENTHOOD RES
System for bright field image simulation
Owner:F HOFFMANN LA ROCHE & CO AG
Test paper box capable of detecting escherichia coli O157:H7
The invention relates to an immunochromatography test paper box based on a thallus fluorescence staining technology and used for detecting a stained escherichia coli O157:H7 thallus by using a single antibody. The immunochromatography test paper box comprises a single antibody test strip and a fluorescence dye box. The immunochromatography test paper box has the advantages that the escherichia coli O157:H7 thallus is pre-stained by using a fluorescence dye, and a specific antibody of the escherichia coli O157:H7 is sprayed on a test strip T line to capture the escherichia coli O157:H7 thallus in a mixed sample, so that the thallus in the sample is rapidly and specifically detected.
Owner:NANCHANG UNIV
Thiazole orange cyanine dye molecule and application thereof
ActiveCN104194379AEffective penetrationImprove bindingMethine/polymethine dyesMicrobiological testing/measurementStainingThiazole
The invention discloses thiazole orange cyanine dye molecules and application thereof. The dye molecules structurally comprise binding groups, ELISA groups, crosslinking groups and connecting parts. The molecules are applied to cell fluorescence staining and are used for judging the living states of bacteria based on the bacterium esterase activity and used for selectively inhibiting the amplification of bacterium DNA in PCR reaction, so that that the purpose of rapidly and quantitatively detecting living bacteria is achieved. The dye molecules disclosed by the invention are simple in structure, low in preparation cost and high in esterase degradation rate.
Owner:SOUTH CHINA UNIV OF TECH
Method for observing deposition of callose of Agrostis stolonifera blade tissues based on paraffin slice and aniline blue fluorescence staining technology
InactiveCN106198465AShorten the experiment timeComplete efficientlyPreparing sample for investigationFluorescence/phosphorescenceANILINE BLUECallose
The invention discloses a method for observing the deposition of callose of Agrostis stolonifera blade tissues based on a paraffin slice and an aniline blue fluorescence staining technology. The method comprises the following steps: 1, preparing a medicine; 2, making the paraffin slice sequentially through the steps of material drawing, fixation, dehydration, transparentizing, paraffin impregnation, embedding, slicing, slice flatting, slice drying, staining and slice sealing, staining the obtained slice by using a fluorescence staining agent, and observing and shooting the stained slice under a fluorescence microscope. The method for observing the deposition of callose of Agrostis stolonifera blade tissues based on the paraffin slice and the aniline blue fluorescence staining technology greatly shortens the experiment time of Agrostis stolonifera paraffin slice production, overcomes the difficulty in the production of the small and fine slice of an Agrostis stolonifera blade, breaks restriction brought by the material and environment factors, rapidly and highly-efficiently completes the paraffin slice production process, and finally obtains an excellent callose fluorescence microscopic observation result.
Owner:GANSU AGRI UNIV
Corneal ulcer classification detection method and system based on neural network model
PendingCN112712122AImprove accuracyImprove efficiencyImage enhancementImage analysisStainingImage detection
The invention discloses a corneal ulcer classification detection method and system based on a neural network model, and relates to the field of corneal ulcer classification detection and disease discrimination. The method mainly comprises the following steps: collecting cornea images of a testee after fluorescence staining at multiple angles by utilizing a plurality of cameras; making and adding a mask to the cornea image, and extracting an effective part; further processing the extracted effective cornea image, and taking the effective cornea image as network input; using the Inception-ResNet-V2 neural network model which is trained by a large amount of effective data to complete preliminary classification of spotting type corneal ulcer, spotting sheet-shaped mixed type corneal ulcer and sheet-shaped corneal ulcer; and processing cornea image detection results of different angles by using the trained support vector machine, and making a final classification judgment. The system provided by the invention can realize high-accuracy corneal ulcer type detection, and data can be uploaded to the cloud server for further analysis.
Owner:HANGZHOU DIANZI UNIV
Application of gold-silver mixed-metal cluster compound in preparing fluorescence staining reagent for cell nucleolus
InactiveCN102798561ARich structure-activity relationshipRapid specific stainingPreparing sample for investigationFluorescenceNucleolus
Application of gold-silver mixed-metal cluster compound in preparing fluorescence staining reagents for cell nucleoli relates to a fluorescence staining reagent. The invention provides an application of the gold-silver mixed-metal cluster compound which has excellent specific staining effect to cell nucleolus area quickly and stably, in preparing fluorescence staining reagents. The gold-silver mixed-metal cluster compound [Au6Ag2(C)(dppy)6](BF4)4 is synthesized using the method in the document: Jian-Hua Jia, Quan-Ming Wang; J. Am. Chem. Soc. 2009, 131, 16634-16635. The dppy is diphenyl-2-pyridylphosphine. The gold-silver mixed-metal cluster compound has fluorescence staining effect to cell nucleolus area, wherein the cell nucleolus fluorescence staining performance of the gold-silver mixed-metal cluster compound reaches or exceeds that of existing like products, so the compound can be used for preparing fluorescence staining reagents for cell nucleoli.
Owner:XIAMEN UNIV +1
A fungus fluorescence staining liquid and a staining method thereof
InactiveCN106244664ALow fluorescent backgroundMicrobiological testing/measurementDiseaseCleansing Agents
A fungus fluorescence staining liquid is disclosed and comprises an A staining liquid and a B staining liquid. The A staining liquid includes 0.03-0.1 part by weight of fluorescein, 1-5 parts by weight of a cleaning agent and 80-150 parts by weight of water. The B staining liquid includes 0.3-1 part by weight of dye and 80-150 parts by weight of water. The A staining liquid can combine cell wall beta-polysaccharides of various funguses, thus labeling and fluorescing for detection. The B staining liquid is adopted as a counterstaining liquid and used for reducing the fluorescence background. The fungus fluorescence staining liquid comprising the A staining liquid and the B staining liquid is a rapid fungal infection detection product and can combine various funguses to emit fluorescence, thus detecting whether various suspected fungal infection diseases are fungal infection or not.
Owner:石家庄博洋生物科技有限公司
Reagent kit for detecting colorectal cancer on basis of liquid biopsy
The invention provides a reagent kit for detecting colorectal cancer on the basis of liquid biopsy. The reagent kit comprises staining enhancement solution for enhancing staining effects and specific antibodies with fluorescence staining markers. The specific antibodies include CK20 antibodies, CD45 antibodies and CDX2 antibodies; the staining enhancement solution comprises surfactants with the concentration of 0.001-1 mg / mL. The reagent kit has the advantages that target cells can be effectively enriched, and whether the enriched target cells come from early-stage patients who suffer from the colorectal cancer or not can be confirmed; the tumor detection sensitivity can be improved by means of double-tumor-marker detection, and the detection accuracy further can be guaranteed by means of CEP8 detection; the staining effects can be enhanced by the staining enhancement solution, accordingly, the diversified antibodies with the fluorescence staining markers can be combined with the target cells, the target cells can be stained, the good staining effects can be realized, fluorescence is intensive, and boundaries are clear.
Owner:SHANGHAI YH HEALTH BIOLOGY MEDICINE TECH CO LTD
Nucleated red blood cell analysis system and method
InactiveUS9103759B2Bioreactor/fermenter combinationsBiological substance pretreatmentsRed blood cellWhite blood cell
Systems and methods for analyzing blood samples, and more specifically for performing a nucleated red blood cell (nRBC) analysis. The systems and methods screen a blood sample by means of fluorescence staining and a fluorescence triggering strategy, to identify nuclei-containing particles within the blood sample. As such, interference from unlysed red blood cells (RBCs) and fragments of lysed RBCs is substantially eliminated. The systems and methods also enable development of relatively milder reagent(s), suitable for assays of samples containing fragile white blood cells (WBCs). In one embodiment, the systems and methods include: (a) staining a blood sample with an exclusive cell membrane permeable fluorescent dye; (b) using a fluorescence trigger to screen the blood sample for nuclei-containing particles; and (c) using measurements of light scatter and fluorescence emission to distinguish nRBCs from WBCs.
Owner:ABBOTT LAB INC
Fungus fluorescence staining method and application thereof
InactiveCN104894211AEasy to operateDoes not affect the dyeing effectMicrobiological testing/measurementPreparing sample for investigationWater bathsStaining
The invention discloses a fungus fluorescence staining method. The method includes firstly, spraying a staining solution [dissolving direct yellow 96 in a 0.1M Tris-Hcl buffer solution with the pH (potential of hydrogen) of 8.5] on the surfaces of host plant leaves uniformly directly to observe conditions of pathogenic fungal spores on the surfaces of the host plant leaves; secondly, soaking leaves decayed by pathogenic fungal hyphae into 95% (v / v) ethyl alcohol in boiling water bath to remove chlorophyll completely; thirdly, taking out the leaves, and cleaning the leaves by 50% (v / v) ethyl alcohol, 50mM sodium hydroxide and pure water sequentially; finally, soaking the leaves into the staining solution to stain so as to observe the conditions after pathogenic fungi invade host leaf tissues. The fungus fluorescence staining method has the advantages that all reagents used in the method are poisonless and harmless and are safer and more environment friendly than reagents such as chloroform, phenol, trichloroacetic acid and pyridine used in conventional decoloration methods; the fungus fluorescence staining method is simple and feasible, and a slicing process is omitted and staining steps are simplified on the premise of not affecting the staining effect.
Owner:HUNAN AGRICULTURAL UNIV
LAMP (loop-mediated isothermal amplification) detection primers for microsporidia in silkworm eggs and application thereof
InactiveCN104372082AEliminate complex and time-consuming steps of separationHigh detection sensitivityMicrobiological testing/measurementAgainst vector-borne diseasesBiotechnologyStaining
The invention discloses an LAMP (loop-mediated isothermal amplification) detection primer group for microsporidia in silkworm eggs and an application thereof. The primer group comprises outside primers EB1-F3 / EB1-B3 and inside primers EB1-FIP / EB1-BIP, wherein the sequences of the primers are shown in SEQIDNO:1-4. An LAMP detection method and kit for microsporidia in silkworm eggs are established by utilizing the primers. The kit comprises the primer group, 2*reaction buffer, positive control substances, negative control substances, a developing liquid (or a fluorescence staining liquid), Bst DNA (deoxyribonucleic acid) polymerase, a sealing liquid and sterile water. The results of detection carried out by adopting the method can be visually observed in natural light or be observed through agarose gel electrophoresis or be observed and determined via a real-time fluorescence curve. The method is easy to operate, has the advantages of short detection time, easiness in determination of results and strong specificity, and can be used for detecting the DNA, at a concentration of 5.0*10<-3>ng / mu L, of the eggs laid by the silkworms infected with nosema bombycis.
Owner:SOUTH CHINA AGRI UNIV
Reagent kit for detecting breast cancer on basis of liquid biopsy
ActiveCN106834511ARemove cleanHigh enrichment efficiencyMicrobiological testing/measurementFluorescenceWilms' tumor
The invention provides a reagent kit for detecting breast cancer on the basis of liquid biopsy. The reagent kit comprises staining enhancement solution for enhancing staining effects and specific antibodies with fluorescence staining markers. The specific antibodies include ER alpha antibodies, VIM antibodies, CD45 antibodies and HER2 antibodies; the staining enhancement solution comprises surfactants with the concentration of 0.001-1 mg / mL. The reagent kit has the advantages that target cells can be effectively enriched, whether the target cells come from early-stage patients who suffer from the breast cancer or not can be confirmed, and tumor can be classified; the tumor detection sensitivity can be improved by means of three-tumor-marker detection, and the detection accuracy further can be guaranteed by means of CEP8 detection; the staining effects can be enhanced by the staining enhancement solution, accordingly, the diversified antibodies with the fluorescence staining markers can be combined with the target cells, the target cells can be stained, the good staining effects can be realized, fluorescence is intensive, and boundaries are clear.
Owner:上海美吉医学检验有限公司
Method for staining tissue
ActiveUS20150064717A1High quantitativityImprove accuracyPreparing sample for investigationMaterial analysis by optical meansTissue stainingFluorescence
Provided is a method for staining a tissue enabling highly precise staining, by which the expression amount and / or the location of a biological substance in a tissue sample can be detected with a high quantitativity together with detailed information that can be obtained by bright field observation.The tissue staining method of the present invention is a method for staining a tissue, in which both staining that allows bright field observation and fluorescence staining are carried out for the same specific biological substance.
Owner:KONICA MINOLTA INC
Application of plantamajoside in preparation of anti-myocardial hypertrophy drugs
ActiveCN108888626AProtectiveInhibition hasOrganic active ingredientsCardiovascular disorderMedicineLeft ventricular size
The invention belongs to novel application of plantamajoside in resistance of myocardial hypertrophy and particularly relates to application of plantamajoside in preparation of anti-myocardial hypertrophy drugs. An isoproterenol is adopted for inducing a myocardial hypertrophy model of a mouse and an H9c2 cardiomyocyte hypertrophy model, and meanwhile, intervention is carried out on plantamajosidedifferent in concentration; cardiac functions of the mouse are evaluated through ultrasound, the heart of the mouse and the left ventricular are weighed, and the myocardial hypertrophy degree of themouse is evaluated. The area of H9c2 cardiomyocytes is observed by means of fluorescence staining, and results show that plantamajoside has a protection effect on isoproterenol-induced myocardial hypertrophy of the mouse and an inhibitory effect on isoproterenol-induced H9c2 cardiomyocyte hypertrophy. Plantamajoside is expected to be developed for clinical use in the anti-myocardial hypertrophy drugs.
Owner:GENERAL HOSPITAL OF THE NORTHERN WAR ZONE OF THE CHINESE PEOPLES LIBERATION ARMY
Rapid Antibiotic Susceptibility Test Using Membrane Fluorescence Staining and Spectral Intensity Ratio Improved by Flow Cytometry Dead to Live Population Ratio
ActiveUS20190017090A1Sure easyReduce incubation timeMicrobiological testing/measurementSpectral emissionFluorescence
Single dye fluorescent staining and the combination of differences in both intensity and spectral emission permit determination of the minimum concentration of an antibiotic needed to inactivate bacteria (Minimum Inhibitory Concentration (MIC)), thereby providing a means for rapid Antibiotic Susceptibility Testing (AST). This allows for a quick and easy means for clinicians to determine a suitable treatment regimen for patients suffering from bacterial infections and those that eventually lead to sepsis.
Owner:POCARED DIAGNOSTICS
Full-automatic mycobacterium tuberculosis microscanning analyzer
PendingCN111272659AFully automatedReduce labor intensityMaterial analysis by optical meansMicroscopesMicroscopic imageStaining
The invention discloses a full-automatic mycobacterium tuberculosis microscanning analyzer which comprises a rack, a slide conveying device, a slide rack, a scanning device, a nozzle clamping mechanism, an optical instrument, a computer and an oil injection system; according to the invention, the slide conveying device is adopted to realize extraction and storage of slide samples between the sliderack and the objective table; a scanning device is adopted to accurately send the sample to a detection position specified by an optical instrument, and microscopic scanning is carried out; a computer and a PLC (Programmable Logic Controller) are used for controlling a first three-dimensional driving power head, a second three-dimensional driving power head and a clamping nozzle driving head to operate and controlling switching of a fluorescence switcher on a fluorescence-Candida mechanism, realizing scanning of Ziehl-Neelsen acid-fast staining and fluorescence staining slides, and operational analysis of microscopic images is performed by adopting a computer and a CPU (Central Processing Unit) to finish full-automatic microscopic scanning work of a detected slide sample. The full-automatic mycobacterium tuberculosis microscanning analyzer has the advantages of high automation degree, large slide rack capacity and high image scanning precision.
Owner:EAST CHINA NORMAL UNIV +1
Application of 2-bromopalmitate in preparation of drugs for prevention and treatment of bone loss related diseases
InactiveCN111789833AInhibition of differentiationAlleviate the pathological process of bone lossAntipyreticAnalgesicsDiseaseStaining
The invention discloses application of 2-bromopalmitate (2-BP) in preparation of drugs for prevention and treatment of bone loss related diseases, and belongs to the technical field of medicine. The invention, for the first time, clearly defines that 2-BP can inhibit osteoclast differentiation process of BMMs stimulated by RANKL through various experimental techniques, including tartrate resistantacid phosphatase staining, phalloidin fluorescence staining, bone lamella bone resorption function evaluation, reverse transcription real-time fluorescence quantitative PCR, Western blot, etc. In addition, the invention clearly states that 2-BP also has effects in inhibiting osteoclast differentiation and activation in vivo, and 2-BP can significantly alleviate the pathological process of massivebone loss caused by estrogen deficiency through the establishment of a mouse model of ovariectomy-induced osteoporosis, thus providing experimental data basis in vivo for the role of 2-BP in the prevention and treatment of bone loss related diseases.
Owner:SUZHOU UNIV
Immunoassay method and kit for assaying mycobacterium tuberculosis from biological samples
The invention discloses a method and a kit for quickly assaying mycobacterium tuberculosis from blood or cerebrospinal fluid by means of fluorescence staining.Particularly, anti-mycobacterium tuberculosis antibodies, especially monoclonal or polyclonal antibodies of ESAT-6 and CFP-10 antigens in RD-1 (region of difference-1) are used for assaying the mycobacterium tuberculosis from biological samples enriched by a microporous membrane-based enrichment device through an indirect or direct immunization method or an SPA method.The method has the advantages of simplicity in operation, high sensitivity, high specificity and capability of distinguishing characteristics of pathogenic mycobacterium tuberculosis and non-pathogenic mycobacterium tuberculosis caused by Bacillus Calmette-Guerin vaccination, can be used for pathogenic detection of tuberculosis diseases and early rapid clinical diagnosis and is a specific tuberculosis etiology diagnostic method.
Owner:肖乐义
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