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57 results about "Direct yellow" patented technology

Fungus fluorescence staining method and application thereof

The invention discloses a fungus fluorescence staining method. The method includes firstly, spraying a staining solution [dissolving direct yellow 96 in a 0.1M Tris-Hcl buffer solution with the pH (potential of hydrogen) of 8.5] on the surfaces of host plant leaves uniformly directly to observe conditions of pathogenic fungal spores on the surfaces of the host plant leaves; secondly, soaking leaves decayed by pathogenic fungal hyphae into 95% (v/v) ethyl alcohol in boiling water bath to remove chlorophyll completely; thirdly, taking out the leaves, and cleaning the leaves by 50% (v/v) ethyl alcohol, 50mM sodium hydroxide and pure water sequentially; finally, soaking the leaves into the staining solution to stain so as to observe the conditions after pathogenic fungi invade host leaf tissues. The fungus fluorescence staining method has the advantages that all reagents used in the method are poisonless and harmless and are safer and more environment friendly than reagents such as chloroform, phenol, trichloroacetic acid and pyridine used in conventional decoloration methods; the fungus fluorescence staining method is simple and feasible, and a slicing process is omitted and staining steps are simplified on the premise of not affecting the staining effect.
Owner:HUNAN AGRICULTURAL UNIV
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