The invention relates to an oxidation-resistant disperse dye composition and preparation and an application thereof. The oxidation-resistant disperse dye composition comprises the combination of any 2-4 of a yellow dye shown as a general formula (I), an orange dye shown as a general formula (II), a blue dye shown as a general formula (III) and a red dye shown as a general formula (IV), not more than two types of dyes are shown as each general formula; and the oxidation-resistant disperse dye composition is applied to pre-treatment dyeing of dacron or polyester-cotton blended fabrics. The disperse dye composition can be used for dyeing polyesters and blended fabrics thereof under the strong oxidative condition that the mass concentration of H2O2 is 0.1-0.5 percent, and an obtained fabric has the advantages of stable chromatic light, bright color, neat cloth cover and excellent fabric style; and according to the disperse dye composition, pretreatment and one-bath and one-step dyeing of polyester fiber or blended fabrics thereof are realized, efficiency is increased, production cost is reduced, and dyeing waste water is reduced.