The invention relates to an array substrate and a preparation method thereof, and a display panel. The array substrate comprises a substrate; the spacing layer is located on one side of the substrate, the spacing layer comprises a first surface deviating from the substrate, at least one groove sunken towards the substrate is formed in the first surface, and the groove has a first
orthographic projection on the substrate; the first
metal layer is located on the first surface and comprises a first grid
electrode of a first
transistor; the
active layer is insulated from the first
metal layer and located on the side, away from the substrate, of the first
metal layer, the
active layer comprises an
active structure, the
active structure comprises a channel region, at least part of the channel region and at least part of the first grid
electrode are oppositely arranged, and the
active structure comprises a first part and a second part; the
orthographic projection of the first part on the substrate at least partially covers the first
orthographic projection; the second metal layer is located on the side, away from the substrate, of the
active layer, the second metal layer comprises a first
electrode of the first
transistor, the first electrode of the first
transistor comprises a second surface facing the substrate, and the second surface makes contact with the second part.