A fungus fluorescence staining liquid and a staining method thereof

A fluorescent dyeing and fungal technology, applied in the field of fluorescent dyeing solution, can solve the problem of low applicability of fluorescent dyeing solution detection

Inactive Publication Date: 2016-12-21
石家庄博洋生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In view of this, the present invention provides a fungal fluorescent staining solution and its dyeing method, which can solve the technical problem of low applicability of existing fluorescent staining solutions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Staining solution A includes 0.1g carboxyfluorescein, 5g 3-(N,N-dimethyltetradecylammonium) propanesulfonate and 150g water; Staining solution B includes 1g pontamine sky blue, 0.005g coumarin-type fluorescent Brightener and 80g of water.

[0048] The sample was sputum from a 56-year-old female patient undergoing lung transplantation;

[0049] Add 1 drop of staining solution A on the glass slide containing the sputum sample and stain for 1 min; add 1 drop of staining solution B on the slide and stain for 1 min; cover with a cover glass, wipe off the excess staining solution, and Observe under a fluorescent microscope, and observe the fluorescence emitted by the bacteria.

Embodiment 2

[0051] Dyeing A solution includes 0.03g tetrachlorofluorescein, 1g benzyl dimethyl tetradecyl ammonium chloride and 80g water; Dyeing B solution includes 0.3g media green 17, 0.02g pyrazoline type fluorescent whitening agent and 130g water.

[0052] The sample was urine from a 28-year-old woman;

[0053] Add 1 drop of staining solution A on the slide containing the urine sample, and stain for 3 minutes; then add 1 drop of staining solution B on the slide, and stain for 1 minute; cover with a cover glass, wipe off the excess staining solution, and Observe under a fluorescent microscope, and observe the fluorescence emitted by the bacteria.

Embodiment 3

[0055] Dyeing solution A includes 0.05 g of hexachlorofluorescein, 2 g of glycodeoxycholic acid and 130 g of water; dyeing solution B includes 0.5 g of direct green 26, 0.01 g of pyrazoline type fluorescent whitening agent and 100 g of water.

[0056] The sample is the wound dander of a 9-year-old boy;

[0057] Add 1 drop of staining solution A on the glass slide containing the urine sample, and stain for 2 minutes; add 1 drop of staining solution B on the slide, and stain for 1 minute; cover with a cover glass, wipe off the excess staining solution, and Observe under a fluorescent microscope, and observe the fluorescence emitted by the bacteria.

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PUM

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Abstract

A fungus fluorescence staining liquid is disclosed and comprises an A staining liquid and a B staining liquid. The A staining liquid includes 0.03-0.1 part by weight of fluorescein, 1-5 parts by weight of a cleaning agent and 80-150 parts by weight of water. The B staining liquid includes 0.3-1 part by weight of dye and 80-150 parts by weight of water. The A staining liquid can combine cell wall beta-polysaccharides of various funguses, thus labeling and fluorescing for detection. The B staining liquid is adopted as a counterstaining liquid and used for reducing the fluorescence background. The fungus fluorescence staining liquid comprising the A staining liquid and the B staining liquid is a rapid fungal infection detection product and can combine various funguses to emit fluorescence, thus detecting whether various suspected fungal infection diseases are fungal infection or not.

Description

technical field [0001] The invention relates to the field of fluorescent dyeing liquid, in particular to a fungal fluorescent dyeing liquid and a dyeing method thereof. Background technique [0002] Fungi are a kind of eukaryotic organisms. Tens of thousands of fungi have been discovered, and among these fungi, there are more than 300 species that are pathogenic to humans. With the exception of Cryptococcus neoformans, Candida and fungi, almost all medically significant pathogenic fungi are molds. Fungi have solid cell walls and true nuclei, live in a parasitic or saprophytic manner, typically have both sexual and asexual reproduction, and produce various forms of robes. [0003] Fungi are prone to cause fungal skin diseases, also known as skin mycosis, which refers to a large class of infectious diseases of human skin, mucous membranes, hair and nails and other skin appendages caused by pathogenic fungi. It is divided into two categories: superficial mycosis and deep myco...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/04
CPCC12Q1/04
Inventor 任学波
Owner 石家庄博洋生物科技有限公司
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