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Multiplex fluorescent RT-PCR kit for detection of H7N9 classic strains and highly pathogenic variant strains

A technology of RT-PCR and mutant strains, applied in the direction of microorganism-based methods, microorganism measurement/testing, microorganisms, etc., can solve the problems of low detection efficiency and inability to distinguish between classic strains and mutant strains of H7N9 virus at the same time , to achieve high sensitivity, improve operational convenience, and easy design

Active Publication Date: 2021-03-05
ZHENGZHOU ZHONGDAO BIOTECHNOLOGY CO LTD +1
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Problems solved by technology

At present, most of the H7N9 nucleic acid detection reagents on the market are aimed at the HA gene or NA gene of the H7N9 avian influenza virus alone, and cannot simultaneously identify and distinguish the classic and variant strains of the H7N9 virus, and the detection efficiency is low

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  • Multiplex fluorescent RT-PCR kit for detection of H7N9 classic strains and highly pathogenic variant strains
  • Multiplex fluorescent RT-PCR kit for detection of H7N9 classic strains and highly pathogenic variant strains
  • Multiplex fluorescent RT-PCR kit for detection of H7N9 classic strains and highly pathogenic variant strains

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Embodiment Construction

[0028] A multiplex fluorescent RT-PCR kit for detecting H7N9 classic strains and highly pathogenic variant strains, the kit includes A box and B box. Box A is equipped with adsorption columns, collection tubes, lysate, washing solution, and eluent, and box B is equipped with positive control, negative control, RT-PCR reaction solution A, and RT-PCR reaction solution B.

[0029] There are 2 pairs of primers and 3 MGB probes in the RT-PCR reaction solution B, the primers are used to amplify the HA and NA gene fragments of the H7N9 avian influenza virus respectively, and the probes are used to identify the HA gene of the H7N9 virus and the H7N9 mutant virus respectively. Strain HA gene and NA gene of H7N9 virus.

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Abstract

The invention discloses a multiplex fluorescent RT-PCR kit for detecting H7N9 classic strains and highly pathogenic variant strains, and provides hemagglutinin (HA) specificity for detecting H7N9 virus classic strains and variant strains Gene primer pair and MGB probe, primer pair and MGB probe for detecting H7N9 virus neuraminidase gene (NA), as well as a kit including them and a method for using the kit. The principle of this kit is: use the HA mutant gene of the H7N9 avian influenza virus variant strain as the molecular marker, combined with the conserved neuraminidase (NA) gene, to prepare a multiple fluorescence detection kit for the classic strain of H7N9 virus and the variant strain . This kit can quickly identify classic strains of H7N9 avian influenza virus and highly pathogenic variant strains, providing strong technical support for early warning of H7N9 avian influenza virus mutation, and can be widely used in animal inspection and quarantine, epidemiological investigations , epidemic prevention and control and other fields.

Description

technical field [0001] The invention relates to a test kit for detecting H7N9 influenza virus, in particular to a multiple fluorescent RT for detecting low pathogenic strains (classic strains) and highly pathogenic strains (variant strains) of H7N9 influenza virus -PCR nucleic acid detection kit, which belongs to the technical field of biological product detection. Background technique [0002] Influenza viruses belong to the Orthomyxoviridae family, and the genome is a single-stranded, negative-strand, multi-segmented ribonucleic acid (RNA) molecule. According to the antigenic differences of influenza virus nucleoprotein (NP), influenza viruses can be divided into three types: A, B, and C. Among them, type A influenza virus is the most common influenza virus, with a wide range of hosts, and can infect most mammals and poultry. and wild birds. According to the amino acid differences of hemagglutinin (HA) and neuraminidase (NA) on the surface of influenza virus, influenza A...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/70C12Q1/686C12R1/93
CPCC12Q1/701C12Q2600/156C12Q2600/16
Inventor 陈小鸽张杰任宝红苗银萍王军曾小宇李伟豪杨会锁籍玉川赵林萍余清卫
Owner ZHENGZHOU ZHONGDAO BIOTECHNOLOGY CO LTD
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