The invention discloses a bisphenol A detecting method and detecting kit based on the dual-quenching-group nucleic acid self-assembling technology. Two DNAs of stem loop structures are designed, wherein one DNA of the stem loop structure is used for modifying a fluorophore, and the other DNA of the stem loop structure is used for modifying two quenching groups; and under the condition that bisphenol A exists, loop opening hybridization can be continuously carried out on the two DNAs of the stem loop structures, so that the fluorophore and the quenching groups get close to each other, fluorescence energy transferring happens, and fluorescence is accordingly quenched. According to the detecting method, signal amplification is sourced from continuous loop opening complementation of the two DNAs of the stem loop structures, and no protease is needed to be used, so that operation is easy, a reaction can be completed at the room temperature, the sensitivity is high, and the selectively is good. The two quenching groups exist on one DNA of the stem loop structure at the same time, and the fluorophore on the other DNA of the stem loop structure can be quenched more effectively, so that the fluorescence background is effectively reduced, the detection sensitivity is greatly improved, and the detection limit is 0.4 pM.