Kit for hereditary hearing loss gene detection

A hereditary deafness and gene detection technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve the problems of high price, high cost, complicated operation, time-consuming and laborious operation of special instruments
CN106811533AActive Publication Date: 2017-06-09亚能生物技术(深圳)有限公司
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
亚能生物技术(深圳)有限公司
Publication Date
2017-06-09

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Abstract

The invention relates to the field of gene detection, in particular to a nucleic acid membrane strip and a kit for hereditary hearing loss gene detection. The kit comprises a PCR reaction liquid, wherein the PCR reaction liquid comprises a PCR reaction liquid A, a PCR reaction liquid B, a PCR reaction liquid C, a PCR reaction liquid D and a PCR reaction liquid E and also comprises corresponding primers and probes. According to the kit, the specific amplification LATE-PCR special primers are designed by use of the sequence of various mutation sites, reported by in literature, and the locked nucleic acid modified ZNATM probes are adopted, so that the least tubes of the reaction liquid can detect the most sites. The PCR reaction liquids A, B, C, D and E in the kit can be tested on the same fluorescent PCR instrument by use of the same amplification program, and the clinical requirement for quick and convenient hearing loss detection is satisfied.
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Description

technical field

[0001] The invention relates to the field of gene detection, in particular to a genetic detection kit for hereditary deafness. Background technique

[0002] At present, the existing deafness gene detection products on the market mainly include Boao's nine hereditary deafness-related gene detection kits (microarray chip method) and 15 hereditary deafness-related gene detection kits (microarray chip method); Kaipu's deafness susceptibility gene detection kit (PCR + diversion hybridization method); Zhongsheng Beikong's four deafness gene detection kit (ARMS-PCR method); Jinan Yingsheng's congenital deafness gene detection kit (fluorescence PCR method), drug-induced deafness gene detection kit (fluorescence PCR method), deafness gene GJB2 235delC detection kit (fluorescence PCR method) and PDS gene mutation detection kit (fluorescence PCR method); Zhihai Bioengineering Co., Ltd. Deafness gene mutation detection kit (fluorescent PCR method). Although there are m...

Claims

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