Kit for hereditary hearing loss gene detection

Abstract

The invention relates to the field of gene detection, in particular to a nucleic acid membrane strip and a kit for hereditary hearing loss gene detection. The kit comprises a PCR reaction liquid, wherein the PCR reaction liquid comprises a PCR reaction liquid A, a PCR reaction liquid B, a PCR reaction liquid C, a PCR reaction liquid D and a PCR reaction liquid E and also comprises corresponding primers and probes. According to the kit, the specific amplification LATE-PCR special primers are designed by use of the sequence of various mutation sites, reported by in literature, and the locked nucleic acid modified ZNATM probes are adopted, so that the least tubes of the reaction liquid can detect the most sites. The PCR reaction liquids A, B, C, D and E in the kit can be tested on the same fluorescent PCR instrument by use of the same amplification program, and the clinical requirement for quick and convenient hearing loss detection is satisfied.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a genetic detection kit for hereditary deafness. Background technique [0002] At present, the existing deafness gene detection products on the market mainly include Boao's nine hereditary deafness-related gene detection kits (microarray chip method) and 15 hereditary deafness-related gene detection kits (microarray chip method); Kaipu's deafness susceptibility gene detection kit (PCR + diversion hybridization method); Zhongsheng Beikong's four deafness gene detection kit (ARMS-PCR method); Jinan Yingsheng's congenital deafness gene detection kit (fluorescence PCR method), drug-induced deafness gene detection kit (fluorescence PCR method), deafness gene GJB2 235delC detection kit (fluorescence PCR method) and PDS gene mutation detection kit (fluorescence PCR method); Zhihai Bioengineering Co., Ltd. Deafness gene mutation detection kit (fluorescent PCR method). Although there are m...

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Problems solved by technology

[0004] At present, the kits for detecting non-syndromic hereditary deafness genes clinically used in my country are mainly developed based on the principle of liquid phase chip hybridization method, which realizes the detection of a small number of mutation sites in multiple genes in a multi-tube PCR system For example, the 15 hereditary deafness-related gene detection kits and the deafness susceptibility gene detection kits of Boao Biotechnology Co., Ltd. and Chaozhou Kaipu Biochemical Co., Ltd. can realize the mutations of 15 and 9 kinds of deafness susceptibility genes respectively. site detection, but these methods either require special instruments that are expensive, or have low throughput, or are complex, time-consuming, and labor-intensive

[0005] In addition to the above-mentioned kits based on the liquid-phase chip hybridization method, there is another kit developed based on the principle of fluorescent PCR technology in clinical practice. For example, the kits developed by Zhongsheng Beikong and Jinan Yingsheng Biotechnology Co., Ltd. Detection of a few sites of susceptible genes, especially each kit of Jinan Yingsheng can only detect a few sites of a gene, with few detection sites and low throughput. If a sample needs to be tested at the same time To detect several deafness susceptibility genes, it is necessary to use three kits at the same time, and the cost is high

[0006] At present, there is no kit for detecting multiple mutation sites of multiple deafness susceptibility genes in the Chinese population

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