49results about How to "Increase birth rate" patented technology

The invention discloses a green biological active feed, which relates to the technical field of feeds. The green biological active feed is prepared from the following materials in part by weight: 40 to 80 parts of straw, 5 to 30 parts of corn four, 5 to 25 parts of cake dregs, 5 to 25 parts of wheat bran, 0.1 to 0.2 part of complex bacteria and 0.1 to 0.2 part of complex enzyme. During preparation, the straw is crushed and then is uniformly mixed with the corn flour, the cake dregs and the wheat bran; the complex bacteria and the complex enzyme are mixed into the mixture; water is added into the mixture to ensure that the water content reaches 45 to 50 percent, and the mixture is filled in a big tank, sealed and stored for later use; or the mixture is baked or dried by natural wind for storage and later use. The green biological active feed has the following advantages of: (1) improving the balance of intestinal flora, and improving the feed utilization rate; (2) promoting the growth of meat livestock and poultry, and shortening the breeding cycle; (3) strengthening the body conditions of laying hens, ducks and geese, and having a significant strengthening action on prolonging theegg-laying peak period; (4) strengthening the body condition of female domestic animals, and improving the yielding rate; (5) strengthening the body conditions of the livestock and poultry, enhancingthe disease prevention and disease resisting capacities, and reducing the sickness rate; and (6) purifying the culture environment, and improving the meat quality due to no public nuisances and no residues.

The invention relates to diagnostic reagent of mediterranean anemia, in particular to a nucleic acid membrane strip and kit for alpha and beta mediterranean anemia gene detection. The technical scheme is that the nucleic acid membrane strip for alpha and beta mediterranean anemia gene detection comprises a substrate and a specific oligonucleotide probe fixed on the substrate, wherein the specific oligonucleotide probe comprises 6 normal alpha-mediterranean anemia (3 deletion types and 3 mutant types) probes and 17 mutant type beta-mediterranean anemia probes. By means of the nucleic acid membrane strip and the kit, deflection type alpha-mediterranean anemia, non-deflection type alpha-mediterranean anemia and beta-mediterranean anemia can be detected in one step simultaneously. Compared with the existing like technology, 2 unreported beta-mediterranean anemia (-30 and -32) and 1 unreported non-deflection type alpha-mediterranean anemia (alpha WS alpha) are detected simultaneously, occurrence rate of the three mediterranean anemia in China is not low, the risk of leak detection in poor areas can be greatly increased without detection, and severe burden can be brought to families and the society.

The invention relates to the field of gene detection, in particular to a nucleic acid membrane strip and a kit for hereditary hearing loss gene detection. The kit comprises a PCR reaction liquid, wherein the PCR reaction liquid comprises a PCR reaction liquid A, a PCR reaction liquid B, a PCR reaction liquid C, a PCR reaction liquid D and a PCR reaction liquid E and also comprises corresponding primers and probes. According to the kit, the specific amplification LATE-PCR special primers are designed by use of the sequence of various mutation sites, reported by in literature, and the locked nucleic acid modified ZNATM probes are adopted, so that the least tubes of the reaction liquid can detect the most sites. The PCR reaction liquids A, B, C, D and E in the kit can be tested on the same fluorescent PCR instrument by use of the same amplification program, and the clinical requirement for quick and convenient hearing loss detection is satisfied.

The invention discloses a breeding and a culture method of hybridization pig of Dashishan area wild boar and Guangxi Luchuan pig, and a wild boar from southern Dashishan area is selected as the father and a Guangxi Luchuan pig is selected as the mother for hybridization, obtaining male pigs of a first generation; and the male pigs of the first generation are selected for hybridization with Guangxi Luchuan female pigs to obtain a hybridized pig. The culture method is characterized in that steps like hybridization, domestication, culture environment management, female pigs and piggy breeding management and hog breeding management are included; the wild boar bred in this way has the advantages of strong field survival adaptability, mild temper, strong disease resistance, fast growth speed, good meat quality, crisp leather, little fat meat, 2.3 times of giving birth a year and 10-12 pigs every time, and the forage consumption is 50% less than that by domestic breeding.

It is intended to provide a method for improving a development rate in a somatic nuclear transplantation technique or an artificial insemination technique using a spermatid. The method is a method for producing a nuclear-transplanted egg comprising the steps of transplanting a nucleus of a donor cell into an egg, and treating the nuclear-transplanted egg with an anti-methylating agent.

The invention provides a method for reproducing baby rabbits in burrows. The burrow is provided with an entrance, an exit, a reproduction cavity and cover and a born rabbit tray, which is fabricated in situ or prefabricated, and is placed underground, semi-underground or on the ground at one side of a cage. The female rabbits give birth in the reproduction cavity and the baby rabbits grow and develop in the reproduction cavity. The burrows adopted in the method can simulate the ecological environment of the natural caves so as to be warm in winter and cool in summer and have appropriate humidity, are convenient for repetitive fabricating and factory production, are convenient for cleaning, washing and sterilizing, overcome artificial interference, can improve growth and development as well as growth velocity and health level of the baby rabbits and reduce occurrence of diseases. The female rabbits can give birth for multiple times in the burrows in a year, thus increasing the reproductive output. The method of the invention is suitable for rabbit breeding and reproduction.

The invention discloses a g-C3N4-doped polyimide composite material which is prepared by the following steps: polymerizing a diamine monomer ODA (diaminodiphenyl ether) and another dianhydride monomer PMDA (pyromellitic dianhydride) or BPDA (3,3',4,4'-biphenyl dianhydride) to form polyamic acid, wherein graphite carbon nitride (g-C3N4) is added before the polyamic acid solution formation process; and after the system is imidized, carrying out hot pressing to form the g-C3N4-doped polyimide composite material. The g-C3N4 is doped into the polyimide system under the physical action. The g-C3N4-containing polyimide film material obtained by the method has favorable thermal neutron shielding property. The invention also discloses a preparation method of the g-C3N4-doped polyimide material and application of the g-C3N4-doped polyimide material in neutron radiation shielding.

The invention discloses an optimized mice somatic cell nuclear transplantation method. According to the mice somatic cell nuclear transplantation method, three kinds of cells, including cumulus cell, fetal fibroblasts, and embryonic stem cell, are adopted to construct reconstructed embryos; the activation efficiencies of three reconstructed embryos in different activation mediums are compared, development efficiencies in different solutions are compared, and pregnancy efficiencies of different embryo transplantation methods are compared; it is concluded that calcium-free KSOM activation medium is a universal high efficiency activation medium, and the activation efficiency is about 93.5%; KSOM-AA culture medium is suitable for in vitro culture of the reconstructed embryos of cumulus cells and embryonic stem cells, and the best culture medium for fetal fibroblasts is alpha MEM culture medium; when the reconstructed embryos are at 2-cell period, nuclear transplanted animals can be obtained via transplantation of embryos through bilateral fallopian tubes, wherein for each fallopian tube, transplantation of 15 embryos is carried out. The optimized mice somatic cell nuclear transplantation method is capable of increasing reconstructed embryo cleavage rate, blastula development rate, cloning animal birth rate, and survival rate, so that the success rate of obtaining embryonic stem cells from the reconstructed embryos is increased.

The method for transferring exogenous gene into animal cell includes the following steps: using target gene to construct target gene expression vector, using nuclear localization protein gene to construct nuclear localization protein gene expression vector and transferring the obtained target gene expression vector and nuclear localization protein gene expression vector into the cytoplasm of cultured cell or cytoplasm of animal cylula by means of cotransfection or co-injection or electroporation.

The invention discloses a method for increasing the cloning efficiency by increasing the embryo transplantation lambing percentage and survival rate, belonging to the field of embryo transplantation methods. The method comprises the following steps of: transplanting embryos with the embryonic ages 24-72 hours and uniform cleavage into synchronous estrus female sheep after transplantation activation, wherein 10-15 embryos are transplanted into every sheep; performing pregnancy management on female sheep which do not return to estrus within four continuous sexual periods after surgery; performing caesarean operation on surrogacy female sheep which surpass an expected date of childbirth by 5 days and of which the fetus body forms are too large; strictly controlling the nursing ambient temperature of borne lambs at 21-23 DEG C; and feeding the lambs with colostrum of healthy female sheep. Due to the adoption of the method provided by the invention, the pregnancy rate, lambing percentage and lamb survival rate of cloned sheep can be increased remarkably. After operation is performed in strict accordance with the invention, the pregnancy rate of cloned sheep is up to 30 percent, the lambing percentage is up to 31.67 percent, and the survival rate is up to 63 percent. The method for increasing the sheep cloning efficiency disclosed by the invention has wide application prospect.

The invention discloses bio-fermented feed capable of improving reproductive performance of sows as well as a preparation method and an application of the bio-fermented feed, and belongs to the technical field of feed for the sows. The fermented feed comprises the following raw materials: corn, extruded soybean, Fortide, dietary fiber, fish meal, dehulled soybean meal, emulsified flaxseed oil, glucose, wheat bran, flour, calcium hydrogen phosphate, calcium formate, edible salt, choline chloride, lysine, methionine, threonine, probiotics, a Chinese herbal medicine additive, an acidulant, micromineral elements and vitamins. The bio-fermented feed aims to improve reproductive performance of the sows and reduce diseases in a reproductive process of the sows, provides the sows and fetuses withnutrition supply in the reproductive process, increases parturition rate of the sows and survival rate of the piglets and can shorten post-reproduction recovery time of the sows and excavate reproductive potential of the sows.

The invention provides a breeding method for Du Changque Nei Sanyuan pigs. The original purebred Landrace SPF boar from Denmark is mated with the local purebred Queshan black pig sows to produce second-generation sows. The pigs are mated with the produced second-generation sows, and the offspring produced and bred are Du Changque Nei Sanyuan pigs. After crossbreeding, litter size, daily gain, lean meat percentage and feed conversion rate were all significantly improved.

The invention provides application of a KSOM-AA culture solution to in-vitro culture of NOD background mouse embryos. By comparing with the effects of different culture solutions in culture of the NODbackground mouse embryos, the invention finds that the KSOM-AA culture solution is suitable for in-vitro culture of the NOD background mouse embryos, especially NCG background mouse embryos and NSG background mouse embryos; and with the cooperation of the constant temperature condition of 35-38 DEG C, the cleavage rate is reduced, the blastocyst formation rate is increased, the 2 cell developmentrate can reach 97.30%, and the blastocyst development rate can reach 18.92%; The KSOMAA culture solution is utilized to culture the NOD, NCG and NSG background mouse embryos, so that the implantationcapacity of the embryos is improved, thereby improving the birth rate; and the KSOM-AA culture solution has important significance in the aspect of in-vitro culture of the NOD, NCG and NSG backgroundmouse embryos.

The invention belongs to the biomedical field, and discloses a drug use of acetylcholine (ACh) or an acetylcholinesterase inhibitor in treatment of fetal hypotension. Through intravenous injection of ACh and monitoring of blood pressure changes, ACh is found out to be able to slightly improve fetal blood pressure and heart functions. Therefore, the invention aims to provide the new use of ACh, namely a therapy method for applying ACh to treat fetal hypotension caused by intrauterine adverse environments so as to improve fetus oxygen resistance, and the fetal all-organ damage caused by hypoxia is reduced. A new drug developed according to the curative effect, obtained by direct modification of ACh and indirectly improving the content of in-vivo ACh is expected to slow down fetal intrauterine distress and improve the birth rate of healthy newborns.

The invention discloses a method for improving mammal cloning efficiency based on acquisition of specific donor cells. According to the method, the donor cells with low-level DNA (deoxyribonucleic acid) methylation in XIST-DMR (X-inactive specific transcript-deuteron magnetic resonance) are acquired and used for nuclear transfer experiments, so that the mammal cloning efficiency is improved. Cloned embryos generated by the method for improving the mammal cloning efficiency based on acquisition of the specific donor cells are lower in XIST-DMR DNA methylation level at a blastula stage, so thatthe development birth rate of the cloned embryos is remarkably increased.

The invention discloses a preparation method of a special feed for Huai sows, capable of increasing the farrowing rate of sows. Based on a fermented soybean meal feed, a compound bacterium agent is used for fermenting a Chinese herbal medicine complete feed, part of feed protein is decomposed into small peptides and free amino acids, and sour fragrance is provided for a fermented feed, so that the palatability is improved, the feeding is promoted, the digestibility and the digestive ability of the feed are improved, and the growing performance of the sows is improved. The special feed has the effects in the respects of advanced sexual maturity, flushing, rapid postnatal oestrus and the like, the propagation rate is increased, and Chinese herbal medicines have the advantages of being low in toxicity, free from drug resistance, economical, practical, multifunctional and the like. The conversion rate, the bacteria resistance and the worm expelling capacity of the feed are notably increased, the production performance of the sows is improved, and the tolerance of the sows is improved. According to the feed for the sows, provided by the invention, the nutrient level of the sows can be effectively increased, the intestinal flora balance can be improved and maintained, the immunity and the disease resistance can be improved, the feed is free from antibiotics, green and safe, the farrowing rate of the Huai sows can be notably increased, and the breeding gain can be increased.

The invention relates to a transgenic rabbit with an enhanced green fluorescent protein gene marker, and a construction method thereof. EGFP (Enhanced Green Fluorescent Protein) expressed by the transgenic rabbit marks all cells of a rabbit individual; green fluorescent is emitted under the excitation of blue light, and a prominent genetic marker sample is used for distinguishing a non-marker sample. The construction method comprises the following steps: introducing a recombinant EGFP coding gene optimized by a promoter into a ribbit fertilized ovum to obtain an EGFP transgenic rabbit; carrying out EGFP gene detection or green fluorescent detection on a baby rabbit, wherein a positive result shows that the baby rabbit is a founder; carrying out hybridization on the founder and a normal rabbit to obtain an EGFP transgenic rabbit strain with genetic stability, and irradiating green fluorescent from rabbit tissues and culture cells subjected to line establishment. A promoter sequence is optimized, the EGFP is applied to manufacture the transgenic rabbit, and the rabbit marked by the EGFP can provide a visually visible biomarker sample from a macroscopic (individual) level to a microcosmic (cell) level.

The invention relates to an automobile windshield wiper blade. The automobile windshield wiper blade comprises an adhesive tape body located on the outer side, wherein the end face of the adhesive tape body is inverted cone-shaped; a highly wear-resistant friction strip is arranged at the inner side top end part, in surface contact with glass, of the adhesive tape body. Moreover, the invention further provides extrusion molding equipment of the automobile windshield wiper blade and a manufacturing method of the automobile windshield wiper blade. The automobile windshield wiper blade disclosed by the invention is novel in structure, low in manufacturing cost and stable in product performance; the discharge amount can be controlled more accurately, the waste of burrs is reduced, the material supply is stable, and the production efficiency, stability and yield of the product are improved.

The present disclosure provides methods of generating multiplexed genetically modified animals, for example, porcine endogenous retrovirus (PERV)-inactivated pigs. The disclosure also provides methods of improving the birth rate of multiplexed genetically modified animals. In some embodiments, the present closure is concerned with the generation and utilization of porcine cells in which porcine endogenous retroviral (PERV) elements have been inactivated. In sonic embodiments, the PERV-free or PERV-reduced porcine cells are cloned to produce porcine embryos. In some embodiments, the PERV-free or PERV-reduced embryos may be grown into adult swine from which organs and / or tissues may be extracted and used for such purposes as xenotransplantation into non-porcine animals such as humans.

The invention relates to diagnostic reagent of mediterranean anemia, in particular to a nucleic acid membrane strip and kit for alpha and beta mediterranean anemia gene detection. The technical scheme is that the nucleic acid membrane strip for alpha and beta mediterranean anemia gene detection comprises a substrate and a specific oligonucleotide probe fixed on the substrate, wherein the specific oligonucleotide probe comprises 6 normal alpha-mediterranean anemia (3 deletion types and 3 mutant types) probes and 17 mutant type beta-mediterranean anemia probes. By means of the nucleic acid membrane strip and the kit, deflection type alpha-mediterranean anemia, non-deflection type alpha-mediterranean anemia and beta-mediterranean anemia can be detected in one step simultaneously. Compared with the existing like technology, 2 unreported beta-mediterranean anemia (-30 and -32) and 1 unreported non-deflection type alpha-mediterranean anemia (alpha WS alpha) are detected simultaneously, occurrence rate of the three mediterranean anemia in China is not low, the risk of leak detection in poor areas can be greatly increased without detection, and severe burden can be brought to families and the society.

The invention provides a rabbit non-operative embryo transfer instrument, a method and an application thereof. The transfer instrument comprises an outer tube sleeve, an outer tube, an inner tube anda syringe. The rabbit embryo was inserted into the inner tube. Binding recipient rabbit, inserting an outer tube with an outer tube sleeve from the recipient rabbit's vagina until the tip of the outertube reaches the cervix of uterus; Pull out the outer sleeve outward so that the closed end of the outer sleeve is pierced by the tip of the outer sleeve. The outer tube is pushed so that the tip ofthe outer tube passes through the cervix, the inner tube is inserted into the tip of the outer tube, and the rabbit embryo in the inner tube is injected into the uterus with a syringe connected with the inner tube. The invention eliminates the injury and infection risk of the recipient rabbit caused by the operation, can quickly transfer the rabbit embryo into the recipient rabbit uterus, can improve the efficiency of the rabbit embryo transfer and the fetal birth rate, and is widely applied.

A method for cultivating a fragrant beaver variety, 1. Select a beaver that is large in size, strong in activity, thick and soft in fluff, and strong in cold regions, and selects a beaver with a high reproductive rate, a strong body shape, a long tail, a large sachet, and a strong fragrance. The Mongolian subspecies with good omnivorousness were crossed with domesticated beavers in the south; (2) Using the pedigree selection method, after several generations of elimination and breeding, a large body, good omnivorousness, high childbirth rate, large sachet, and It is a species of civet with a strong aroma and a large and long tail. After epidemic prevention treatment, it is bred in zoos or specific farms. The tail is used as a medical suture, the sachet is extracted as a spice, and its fur can also be used.

The invention discloses a health-caring method during a black pig maternal fertility phase. The method comprises steps of selecting a culturing case with an area of 0.5 to 1 square meters, disinfecting and exposing the culturing case under the sun, controlling the temperature of the culturing case at 18 DEG C to 25 DEG C and relative humidity of 40 t o50% for future use, in the fertile period of the black pig parent body, before black pig maternal pregnancy, feeding first forge and first green material to the black pig parent body, in a gestation period of the black pig parent body, after successful black pig parent body copulation, feeding second forge and second green material to the black pig parent body, stopping feeding the second forge 3 to 5 days before labor, and feeding earthworm hygromel in each morning, during a lactation period of the black pig parent body, exchanging the second forge to third forge, and the second green material to third green material. Immunity of the black pig parent body can be enhanced, parturition rate of the black pig parent body can be increased and post-labor recovery period of the black pig parent body can be shortened.

The method for transferring exogenous gene into animal cell includes the following steps: using target gene to construct target gene expression vector, using nuclear localization protein gene to construct nuclear localization protein gene expression vector and transferring the obtained target gene expression vector and nuclear localization protein gene expression vector into the cytoplasm of cultured cell or cytoplasm of animal cylula by means of cotransfection or co-injection or electroporation.

The invention discloses a breeding and a culture method of hybridization pig of Dashishan area wild boar and Guangxi Luchuan pig, and a wild boar from southern Dashishan area is selected as the father and a Guangxi Luchuan pig is selected as the mother for hybridization, obtaining male pigs of a first generation; and the male pigs of the first generation are selected for hybridization with Guangxi Luchuan female pigs to obtain a hybridized pig. The culture method is characterized in that steps like hybridization, domestication, culture environment management, female pigs and piggy breeding management and hog breeding management are included; the wild boar bred in this way has the advantages of strong field survival adaptability, mild temper, strong disease resistance, fast growth speed, good meat quality, crisp leather, little fat meat, 2.3 times of giving birth a year and 10-12 pigs every time, and the forage consumption is 50% less than that by domestic breeding.

The invention provides a culture method of mammal embryonic cells. During culture, uterine fetal fluid of mammals during pregnancy is not added; the culture is divided into two stages, and during the two stages, culture times and medium formulas are different. The culture method provided by the invention is suitable for culture of the embryonic cells of the multiple mammals and has higher cleavage rate, fusion rate and blastocyst rate. After an embryo obtained by the culture method provided by the invention is directly transplanted, the birth rate is high.

The invention relates to a transgenic rabbit with an enhanced green fluorescent protein gene marker, and a construction method thereof. EGFP (Enhanced Green Fluorescent Protein) expressed by the transgenic rabbit marks all cells of a rabbit individual; green fluorescent is emitted under the excitation of blue light, and a prominent genetic marker sample is used for distinguishing a non-marker sample. The construction method comprises the following steps: introducing a recombinant EGFP coding gene optimized by a promoter into a ribbit fertilized ovum to obtain an EGFP transgenic rabbit; carrying out EGFP gene detection or green fluorescent detection on a baby rabbit, wherein a positive result shows that the baby rabbit is a founder; carrying out hybridization on the founder and a normal rabbit to obtain an EGFP transgenic rabbit strain with genetic stability, and irradiating green fluorescent from rabbit tissues and culture cells subjected to line establishment. A promoter sequence is optimized, the EGFP is applied to manufacture the transgenic rabbit, and the rabbit marked by the EGFP can provide a visually visible biomarker sample from a macroscopic (individual) level to a microcosmic (cell) level.