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A rabbit non-surgical embryo transfer instrument

A technology of embryo transfer and equipment, which is applied in the field of non-surgical embryo transfer equipment for rabbits, can solve the problems of non-surgical embryo transfer methods that have not been reported, and achieve the effects of reducing costs, benefiting embryo development, and reducing damage

Active Publication Date: 2020-06-19
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Recent studies have reported the successful method of non-surgical embryo transfer in mice, but there is no research report on the method of non-surgical embryo transfer in rabbits

Method used

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  • A rabbit non-surgical embryo transfer instrument
  • A rabbit non-surgical embryo transfer instrument
  • A rabbit non-surgical embryo transfer instrument

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Embodiment Construction

[0027] The present invention will be described in detail below in conjunction with the drawings and embodiments, and the embodiments are used to explain the present invention rather than limit the present invention.

[0028] (1) Non-surgical rabbit embryo transfer

[0029] 1) Source and preparation of reagents and culture / treatment solutions

[0030] Pituitary follicle-stimulating hormone (PMSG) and chorionic gonadotropin (HCG) for injection were purchased from Ningbo Sansheng Pharmaceutical Co., Ltd. Sterile water for injection was purchased from Ruicheng Weifu Veterinary Medicine Co., Ltd. Sodium pentobarbital was purchased from Merck. DPBS, EBSS and FBS were purchased from Hyclone Company.

[0031] Embryo culture medium: EBSS+10%FBS

[0032] Embryo transfer solution: EBSS+5%BSA

[0033] 2) The specific process of rabbit embryo transfer

[0034] 2.1 Preparation of donor and recipient rabbits

[0035] Preparation of donor rabbits: New Zealand female rabbits, 6 months o...

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Abstract

The invention provides a rabbit non-operative embryo transfer instrument, a method and an application thereof. The transfer instrument comprises an outer tube sleeve, an outer tube, an inner tube anda syringe. The rabbit embryo was inserted into the inner tube. Binding recipient rabbit, inserting an outer tube with an outer tube sleeve from the recipient rabbit's vagina until the tip of the outertube reaches the cervix of uterus; Pull out the outer sleeve outward so that the closed end of the outer sleeve is pierced by the tip of the outer sleeve. The outer tube is pushed so that the tip ofthe outer tube passes through the cervix, the inner tube is inserted into the tip of the outer tube, and the rabbit embryo in the inner tube is injected into the uterus with a syringe connected with the inner tube. The invention eliminates the injury and infection risk of the recipient rabbit caused by the operation, can quickly transfer the rabbit embryo into the recipient rabbit uterus, can improve the efficiency of the rabbit embryo transfer and the fetal birth rate, and is widely applied.

Description

technical field [0001] The invention belongs to the field of experimental animal applications, and relates to a non-surgical transplantation device and method for rabbit embryos used in the process of constructing model animal disease models and the like. Background technique [0002] Rabbit is an important model animal, very close to human in phylogeny. Rabbits are of great value in the research of lipid metabolism, atherosclerosis and other diseases. Interfering with drugs on rabbits, such as feeding rabbits with cholesterol to establish atherosclerosis models, is a very important model for studying lipid metabolism and atherosclerosis. Natural mutation selection to obtain disease model rabbits, such as WHHL and WHHLMI, and rabbits with specific genetic modifications through gene editing, which play an important role in elucidating the mechanism of disease, developing new drugs and new treatment options. So far, a variety of gene-edited rabbits have been reported. [00...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61D19/04
CPCA61D19/04
Inventor 刘恩岐屈鹏祥李璐贾林莹
Owner XI AN JIAOTONG UNIV
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