The selenium-rich pagodatree ear mother culture medium consists of selenium-rich fertilizer, potato, agar, glucose, bean powder, magnesium sulfate, zinc sulfate, potassium dihydrogen phosphate, peptone, 6-BA, mulberry leaf or twig, and water. The selenium-rich pagodatree ear original culture medium consists of selenium-rich fertilizer, pagodatree sawdust, hardwood sawdust, wheat bran, corn powder, gypsum, bean powder, magnesium sulfate, zinc sulfate, potassium dihydrogen phosphate, 6-BA, and water, etc. Through preparing selenium-rich pagodatree ear original culture medium and three- stage culture medium, culturing selenium-rich pagodatree ear and further preparation, pagodatree ear selenium polysaccharide is prepared. The present invention has excellent selenium enriching condition, high pagodatree ear resistance, low contamination rate, high output and quality of pagodatree ear selenium polysaccharide, and other advantages.