Method for transferring exogenous gene into animal cell

A technology for exogenous genes and animal cells, which can be applied to other methods of inserting foreign genetic materials, using microinjection methods, and using vectors to introduce foreign genetic materials, etc.

CN1400310AInactive Publication Date: 2003-03-05中国科学院发育生物学研究所
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
Publication Date
2003-03-05
Estimated Expiration
Not applicable · inactive patent

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Abstract

The method for transferring exogenous gene into animal cell includes the following steps: using target gene to construct target gene expression vector, using nuclear localization protein gene to construct nuclear localization protein gene expression vector and transferring the obtained target gene expression vector and nuclear localization protein gene expression vector into the cytoplasm of cultured cell or cytoplasm of animal cylula by means of cotransfection or co-injection or electroporation.
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Description

technical field

[0001] The invention relates to a method for transferring foreign genes into animal cells. technical background

[0002] As a technical platform, transgenics can not only be used to prepare mammary gland bioreactors, but also have very important significance in disease model animals, improved animal breeds, and the study of individual development mechanisms at the molecular level. As a bioreactor, transgenic animals can produce medicinal and rare proteins that cannot be obtained by other production methods, and have the characteristics of high efficiency, low cost and more natural products. Its potential economic and social value has been valued by countries all over the world. However, the production of transgenic animals on a large scale has remained difficult for more than a decade. One of the major hurdles is the bottleneck of transgenic methods.

[0003] At present, there are mainly two methods for the preparation of transgenic mammals: pronuclear mic...

Examples

Embodiment Construction

[0014] Our laboratory uses the mouse histone H2AZ (mH2AZ) as a nuclear localization signal to promote the integration of the transgenic construct bovine α-s1-casein-human G-CSF expression vector on the host chromosome, that is, the target gene expression construct and the mH2AZ gene The expression components are co-injected into the fertilized egg cytoplasm, and the target gene expression components are brought into the prokaryon by the directional migration of nuclear localization proteins. The results of this example show that the method used can bring the target gene expression components into the host cell nucleus to obtain transgene integrated mice, and this method is more convenient, practical, and easy to promote, and can bypass the bottleneck of pronuclear injection technology and avoid fertilized eggs. A large number of injuries, promote the widespread application of transgenic animal technology. Therefore, this transgenic approach can completely replace the tradition...