A kind of culture method of mammalian embryonic cell

A technology for mammals and culture methods, applied to animal cells, embryonic cells, vertebrate cells, etc., can solve the problems of uterine fetal fluid interference, differences in culture medium components, unfavorable cost control, etc., and achieve high cleavage rate and high birth rate Effect
CN107365739BActive Publication Date: 2021-03-30SICHUAN AGRI UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
SICHUAN AGRI UNIV
Publication Date
2021-03-30

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Abstract

The invention provides a culture method of mammal embryonic cells. During culture, uterine fetal fluid of mammals during pregnancy is not added; the culture is divided into two stages, and during the two stages, culture times and medium formulas are different. The culture method provided by the invention is suitable for culture of the embryonic cells of the multiple mammals and has higher cleavage rate, fusion rate and blastocyst rate. After an embryo obtained by the culture method provided by the invention is directly transplanted, the birth rate is high.
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Description

technical field

[0001] The invention belongs to the technical field of cell culture, and in particular relates to a method for culturing mammalian embryonic cells. Background technique

[0002] With the development of embryo engineering technology, the study of embryo culture medium has become increasingly important. At present, in the culture of animal embryos, the existing synthetic culture medium (such as TCM199 or mTCM199) contains a high concentration of glucose, which has certain toxicity to early embryos. Therefore, many researchers improved the culture medium by adding serum, amino acids, and growth factors.

[0003] In terms of mouse embryo culture, KSOM culture medium is more mature, which has obtained relatively high blastocyst formation rate and blastomere number.

[0004] However, it has been found that a single culture solution cannot achieve very satisfactory results. Therefore, it has become a research hotspot to cultivate in vitro cultured embryos accordi...

Claims

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