Nucleic acid membrane strip and kit for alpha and beta mediterranean anemia gene detection

Abstract

The invention relates to diagnostic reagent of mediterranean anemia, in particular to a nucleic acid membrane strip and kit for alpha and beta mediterranean anemia gene detection. The technical scheme is that the nucleic acid membrane strip for alpha and beta mediterranean anemia gene detection comprises a substrate and a specific oligonucleotide probe fixed on the substrate, wherein the specific oligonucleotide probe comprises 6 normal alpha-mediterranean anemia (3 deletion types and 3 mutant types) probes and 17 mutant type beta-mediterranean anemia probes. By means of the nucleic acid membrane strip and the kit, deflection type alpha-mediterranean anemia, non-deflection type alpha-mediterranean anemia and beta-mediterranean anemia can be detected in one step simultaneously. Compared with the existing like technology, 2 unreported beta-mediterranean anemia (-30 and -32) and 1 unreported non-deflection type alpha-mediterranean anemia (alpha WS alpha) are detected simultaneously, occurrence rate of the three mediterranean anemia in China is not low, the risk of leak detection in poor areas can be greatly increased without detection, and severe burden can be brought to families and the society.

Description

technical field [0001] The invention relates to a diagnostic reagent for thalassemia, in particular to a nucleic acid membrane strip and a kit for α and β thalassemia gene detection. Background technique [0002] Thalassemia (Thalassemia, hereinafter referred to as thalassemia) is due to the decrease in the synthesis rate of one or some globin chains in the patient, resulting in the lack of some peptide chains and the relative excess of other peptide chains, resulting in hemolysis caused by an imbalance in the number of peptide chains sexual anemia. There are two main types of thalassemia, α-thalassemia caused by a deficiency of α-globin chain synthesis and β-thalassemia caused by a deficiency of β-globin synthesis. [0003] α-thalassemia (including deletion α-thalassemia and non-deletion α-thalassemia) is one of the most common single-gene genetic diseases in the world. Guangxi, Guangdong, Yunnan, Hainan, Hong Kong, Taiwan and other provinces in southern my country The cit...

AI Technical Summary

Problems solved by technology

However, due to the high operating requirements of these products, cumbersome methods, long time-consuming, and high cost due to independent testing, they still cannot meet the requirements of simple and fast clinical diagnosis.

Images