An express transportation packaging box comprises a transportation box body, a box door, a connector unit, a two-dimensional scanning device, an electronic lock unit, an information board, a box door handle and a power source. An opening is formed in one side of the transportation box body, a first bearing face and a second bearing face are longitudinally enlarged in a gradual-changing manner, and locking grooves are formed in the first bearing face and the second bearing face; the box door is arranged on the opening; the connector unit is arranged in a plate shape, and locking columns are arranged at the positions, corresponding to the locking grooves of the transportation box body, of the connector unit; the two-dimensional scanning device is embedded in the box door and provides input information for a single chip microcomputer, and therefore a self-locking switch is controlled; the electronic lock unit further comprises a spring, a connecting rod, a right-angle triangular prism lock head, an iron cylinder and an electromagnet cylinder, the right-angle triangular prism lock head and the iron cylinder are arranged at the two ends of the connecting rod, the electromagnet cylinder and the iron cylinder are jointly arranged in an outer cylinder, and the electromagnet cylinder controls the current to be on and off through the self-locking switch; and according to the express transportation packaging box, multiple boxes can be stacked, the space utilization rate is improved, the box can be recycle, and is energy-saving and environment-friendly, the express transportation efficiency is improved, and the box is worth of application and popularization.