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Thermophilic denitrifying bacillocin, screening and use thereof

A technology of bacillus and heat removal, applied in the direction of bacteria, chemical instruments and methods, methods of using microorganisms, etc., can solve the problem of indirect transportation costs

Inactive Publication Date: 2005-05-11
NANKAI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, if the oil is transported by pipeline, if the fluidity of the oil is poor, means such as heating and pressurization must be used, which consumes a lot of transportation indirect costs

Method used

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  • Thermophilic denitrifying bacillocin, screening and use thereof
  • Thermophilic denitrifying bacillocin, screening and use thereof
  • Thermophilic denitrifying bacillocin, screening and use thereof

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0043] (1) The name and preservation status of the microorganism.

[0044] Bacillus thermodenitrificans G1788 (Geobacillus thermodenitrificans G1788) has been preserved in China General Microbiological Culture Collection Center (China General Microbiological Culture Collection Center), and its preservation number is CGMCC-1228.

[0045] (2) Characterization and identification of strains.

[0046] 1. The morphological, physiological and biochemical characteristics of the G1788 strain.

[0047] According to "Common Bacteria System Identification Manual" (edited by Dong Xiuzhu, Cai Miaoying, etc., Beijing: Science Press 2001.2, ISBN: 7-03-008460-8) and "Bergey's Manual of DeterminativeBacteriology" (Ninth Edition) to G1788 bacteria identification, the results are as follows:

[0048] The cells of the G1788 strain are long rod-shaped, with a size of 0.6-1.0×3.1-6.5 μm, Gram’s staining is positive, flagella are perinatal, and can move; there are spores, spores are terminal or sub...

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Abstract

This invention relates to selection and application of thermophil denitriding Bacillales,of which conservation number is CGMCC-1228. Initial strain is from water of oil field. The strain is obtained by selection, inoculation, and domestication with good thermal stability. It belongs to soil Bacillales and can be used in fermented industry, which needs thermally stable enzyme. It grows well in the oil field and can degrade paraffin and decrease viscosity to improve liquidity. All of this can increase recovery and carriage efficiently. It can be used to control pollution and prepare surfactant.

Description

1. Technical field [0001] The invention relates to microbial strains, in particular to a thermophilic denitrification bacillus G1788 and its screening and application. 2. Background technology [0002] Microbial oil recovery is an emerging field with great application prospects and economic value in microbial application engineering. After primary and secondary oil recovery in an oil field, 60-65% of the crude oil still cannot be recovered. After years of water injection development, the oil field will enter a high water cut period, and will face difficulties such as difficult mining, high cost, and low recovery (such as the main oil fields in eastern my country). Thermal flooding, chemical flooding and miscible flooding, which are commonly used to solve this problem, have certain effects, but each has its serious disadvantages. Thermal flooding has a low utilization rate of thermal energy; chemical flooding and miscible flooding inject chemicals that remain in the formati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C10G32/00C11D3/38C12N1/20C12R1/01
CPCC10G32/00C11D3/381C12N1/20C12N1/26C12Q1/04G01N2333/32C12R2001/01C12N1/205
Inventor 王磊刘如林冯露梁凤来
Owner NANKAI UNIV
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