118 results about "Bacillales" patented technology

The invention discloses bacillus aryabhattai, microbial inoculum thereof, a preparation method and application. The bacillus aryabhattai BC104 is beta-cypermethrin degrading bacteria, and the preservation unit is the China General Microbiological Culture Collection center (CGMCC), the preservation date is 15th, Dec, 2015, and the preservation number is CGMCC No.11664; the bacillus aryabhattai belongs to mycomonera, phylum firmicutes, bacillus class, bacilli bacteria, bacillaceae and bacillus. The microbial inoculum with the bacillus aryabhattai as an active ingredient is the microbial inoculum obtained by preforming activation culture and fermentation culture on the bacillus aryabhattai BC104. The preparation method comprises the steps of activation culture and fermentation culture; the application of the microbial inoculum is to prepare a medicine for degrading beta-cypermethrin. The strain disclosed by the invention has the advantages of simple preparation process of the microbial inoculum, low cost, convenience in use and good application prospect.

The invention relates to a rehabilitation technology of polycyclic aromatic hydrocarbon pollutants (PAHs), particularly discloses a mixed inoculant for degrading PAHs. The mixed inoculant comprises indigenous mixed bacteria, wherein the indigenous mixed bacteria comprise Pseudomonas sp. B08, Pseudomonas sp. BM, Bacillus sp. BYB, Bacillus sp. B07, Ochrobactrum sp. ZHL4, Gordonia sp. BGDS and Mycobacterium sp. BFZG. The mixed inoculant disclosed herein can be added according to an inoculation quantity of 5-10%, and when the initial concentration of PAHs is 180.67mgL<-1>, the total degradation rate of PAHs after being processed for 9 days can reach 91.21%.

Disclosed are bacillus licheniformis engineering bacteria for nattokinase production and a method for producing nattokinase by using the bacillus licheniformis engineering bacteria, which belong to the technical fields of microorganism genetic engineering and enzyme engineering. According to the invention, a nattokinase gene of bacillus subtilis MBS04-6 is obtained through PCR technology augmentation. Bacillus licheniformis BL10 is utilized as an expression host; promoter P43 of bacillus subtilis 168 is taken as a promoter; a signal peptide of extracellular serine protease Vpr of bacillus licheniformis WX-02 is taken a signal peptide; and a terminator sequence of alpha-amylase of bacillus licheniformis WX-02 is taken as a terminator. The expression elements are connected to shuttle vector pHY300PLK to form an expression vector, and the expression vector is transformed into the bacillus licheniformis BL10 to obtain the bacillus licheniformis engineering bacteria BL10 (pP43SNT) for nattokinase production. The bacterial strain has been deposited with the China Center for Type Culture Collection (CCTCC) at September 10th, 2013, and the accession number is CCTCC NO:M2013401. The invention also provides a method of the bacterial strain for producing nattokinase, and the maximum enzyme activity can reach 11.37 FU/mL in a liquid fermentation medium.

The invention discloses a bacillus altitudinis strain and application thereof. The bacillus altitudinis strain is classified and named as Bacillus altitudinis, the preservation unit is the China general microbiological culture collection center (CGMCC), the preservation date is December 28th in 2018, the preservation number is CGMCCNo.17046. The bacillus altitudinis strain belongs to the genus ofbacillus, the family of bacillaceae, the order of bacillales, the class of bacilli, the phylum of firmicutes in the domain of bacteria. The bacillus altitudinis strain can be applied in biological control over root rot of plukenetia volubilis.

The present invention concerns spore display methods. More specifically, the invention concerns display of heterologous molecules, such as peptides and polypeptides, on spores of bacilli, such as, for example, Bacillus thuringiensis (Bt) or Bacillus cereus (BC), using externally exposed spore coat proteins or fragments or variants thereof.

The invention discloses a food-grade nattokinase expression bacterium. The recombinant bacterium is bacillus licheniformis which is transformed so that the genome contains at least one copy nattokinase gene. Preferably, in the genome of the expression bacterium, the negative regulatory factor gene hrcA is inactivated. The production strain provided by the invention accords with the requirements of a food-grade expression system, multiple copy nattokinase genes are tracelessly integrated into the genome of the bacillus licheniformis to be expressed, the expression quantity is obviously improved compared with that in the prior art, through the inactivation of the negative regulatory factor gene hrcA, the enzyme activity and expression quantity are further improved, the enzyme activity achieves 1350FU/mL, and the protein content achieves 1.45g/L. The nattokinase prepared by the recombinant bacterium is high in purity, can be processed into a health product or can be taken as an active ingredient of a medicine for treating cerebral infarction.

The invention belongs to the technical field of agricultural microbiology, and relates to the fields of biochemistry and molecular biology simultaneously. Through separation, a bacillus amyloliquefaciens strain CH1 for producing a growth-promoting fungus apoptosis lipopeptide is obtained, which is preserved in the China Center for Type Culture Collection (CCTCC) with the preserving number of CCTCC: M208127. A bioactive substance is obtained from a culture of the bacillus amyloliquefaciens strain CH1 and is characterized as the growth-promoting fungus apoptosis lipopeptide. The molecular weight of the lipopeptide is 1,065 or a homologue thereof. The lipopeptide has an infrared spectrum as shown in Figure 6 in the instruction and consists of 9 carbon alkyls and 8 amino acids, wherein the seventh alkyl carbon and the last amino acid form a cyclic peptide through an oxygen atom. The bacillus amyloliquefaciens strain and the lipopeptide prepared from the same are tested on the biological application prospect in apoptosis.

The invention discloses a preparation method of Bacillus licheniformis (Bacilluslicheniformis subsp. Licheniformis, BL KN-08, CCTCC: NO. M208251) raw pesticide, comprising the following steps: A. screening B1 strains, collecting samples from soil and water body, adding sterile water, and standing; B. conforming the B1 strain the 16 SrDNA of which totally measures 1514 effective basic groups, and identifying; C. for B1 culture medium, adopting the response surface experiment designed by Box-Behken, taking the amount of spores as a screening index, and using a single factor experiment to select factors from multiple carbon sources, nitrogen sources and microelement factors; D. improving ferment biomass, and using the culture medium of the response surface method for batch fermentation; E. extracting and recovering spores; and F. detecting the amount of live spores. The invention can accurately detect the amount of the effective live spore in a product, has favourable repeatability, low detection cost, simple operation and convenient popularization and can simultaneously detect 7-8 samples; the amount of the live spores in the Bacillus licheniformis raw pesticide of the invention can reach 500 billion/g.

The present invention discloses one kind of human Ochrobactrum anthropi IBL01 (Ochrobactrum anthropi CCTCC M 206046) and its application in degrading plant stalks. Ochrobactrum anthropi IBL01 has high plant stalk degrading effect and can produce high activity cellulase, hadromse and hemicellulase. The present invention also discloses the preparation process of cellulose, xylanase, lignin peroxidase, laccase and manganese peroxidase with the human Ochrobactrum anthropi. The human Ochrobactrum anthropi of the present invention is bacterium with short growth period of about one week, and can produce fiber degrading enzyme as well as hemicellulose degrading enzyme and lignin degrading enzyme.

The invention provides a Bacillus licheniformis engineered bacterium capable of high production of poly-Gamma-glutamic acid, collected under CCTCC No: M2016439 and named as Bacillus licheniformis (WX-02)/pHY-zwf, and further provides a construction method of the Bacillus licheniformis (WX-02)/pHY-zwf and a method for high production of poly-Gamma-glutamic acid. The synthetic level of intracellular NADPH is increased through genetic engineering modification, and the yield of Gamma-PGA. By constructing glucose-6-phosphate dehydrogenase overexpression support pHY-zwf and converting to Gamma-PGA-producing Bacillus licheniformis WX-02 pre-stored in a lab, the Bacillus licheniformis (WX-02)/pHY-zwf capable of producing Gamma-PGA is re-constructed. The bacterium may provide significantly synthesis of Gamma-PGA under optimized media and culture conditions. The yield of Gamma-PGA is up to 51.13 g/L which is 49% significantly higher than the Gamma-PGA yield of an original bacterium.

The invention relates to a Xanthobacter and its snail killer. A snail bacterium, namely Xanthobacter autotrophicus is a Xanthobacter autotrophicus II whose collection unit. It uses beef peptone agar plate or slope culture medium to separate bacterial strains, obtains snail-killing Xanthobacter autotrophicus solution (a hundred million grades/ml) by bottle-shaking fermentation process; and the application of Xanthobacter autotrophicus as a snail killer is that: making ultrasonic processing on the Xanthobacter autotrophicus solution and plant material forming agent, then mixing them to prepare a novel biological snail killer, which has the characters: (1) quickly killing the snail, and effectively preventing the snails from climbing up to flee; (2) harmless to non-target living things and fish, fast to naturally degrade and no pollution; (3) no toxic and side effect, and time of maintaining snail-killing pesticide effect 15 days long; (4) convenient to store, transport and use; etc.

The invention discloses low-temperature denitrification pseudomonas fluorescens and relates to pseudomonas fluorescens. The low-temperature denitrification pseudomonas fluorescens are pseudomonas fluorescens WSW-1001 and are collected in China General Microbiological Culture Collection Center (CGMCC), the collection number is CGMCC No.7476, the collection date is April 15th, 2013, and the collection address is No.3, No.1 Courtyard, Beichen Road, Chaoyang District, Beijing. The low-temperature denitrification pseudomonas fluorescens are heterotrophic nitrification aerobic denitrifiers, can be used for degrading NH4+-N, NO--N and NO--N (0.25 mg/L-1314.1 mg/L) with a wide concentration range, can be applied to low-nitrogen pollution source water denitrification, medium- and high-concentration wastewater denitrification and eutrophic water body treatment, and have an important meaning on low-nitrogen pollution source water denitrification, water eutrophication control and biological environment modification.

A drug-resistance gene film chip for detecting mycobacterium tuberculosis is prepared by designing 54 probe spotting on a nylon film by aiming at the mutant sites of mycobacterium tuberculosis rpoB, kagG, embB, inhA, ahpC, gyrA, rrs, rpsL and pncA gene; 12 pairs of specific primers with biotin-labeled at 5' end are utilized; a sample DNA is subjected to triple PCR and amplified to form a large amount of gene fragment products with biotin; the amplified products and the probes on the film chip carry out specific hybridization; and then film washing, enzyme-linking and color reaction are carried out, thus preparing the chip. The gene film chip and the detection method thereof can detect the common gene mutations of the mycobacterium tuberculosis on the drug resistance of drugs such as isoniazid, rifampicin, streptomycin, ethambutol, pyrazinamide, quinolone and the like at one step, and are applicable to extracorporeal detection sputum sample, clinical isolation strains and mycobacterium tuberculosis multi-drug resistant gene in the organization sample.

Disclosed is a model human-source flora litter model construction and method for detecting flora in litter intestinal tract. The construction steps contain: (1) prepare healthy people fecal bacteria suspension; (2) paunch to farrowing sow to fetch litters; (3) breed the litters in 24-hour aero-ultra filter positive-pressure draft isolation room; (4) inoculate. The detecting steps contain: (1) extract the fecal microbe genome DNA; (2) analyze ERIC-PCR finger spectrum and community DNA crossing; (3) planting analyze bacillus in HFA litter intestinal tract flora; (4) planting analyze HFA bacteroide in HFA litter tract flora. This invention successfully plant human donator's bacillus and bacteroide in litter model intestinal tract. The finger spectrums comparing shows that the flora finger spectrum in litter model intestinal tract has homologous height with that of the human donator. This invention can be used to study the cross-correlation between intestinal tract flora and its host physiology and immune specificity, also it can be used to evaluate regulation and metabolism effect of functional foods or drugs to intestinal tract flora.

The invention belongs to the technical field of genetic engineering of enzymes, and relates to a novel laccase from bacillus amyloliquefaciens and a gene, preparation method application of the novel laccase. According to the technical scheme, by using a molecular biology means and a genetic engineering technology, the bacillus amyloliquefaciens capable of generating the laccase is obtained throughstrain selection, the gene of the novel laccase is amplified through a PCR technique, and then the gene of the novel laccase is expressed in a bacillus subtillis expression system, a bacillus amyloliquefaciens expression system and a bacillus licheniformis expression system to obtain a bacillus subtillis high-stability laccase recombined strain, a bacillus amyloliquefaciens high-stability laccaserecombined strain and a bacillus licheniformis high-stability laccase free expression recombined strain respectively, and preparation of the novel laccase is achieved. The novel bacterial laccase plays a good role in the aspects of protein polymerization, dye decolorization and paper making.

The invention discloses a Duganella bacterium and its application. The Duganella bacterium is sp. B2 CGMCC No:2056. Culturing Duganella sp. B2 CGMCC No:2056 will get purple bacteriocin. The fermenting condition for the bacterial strain is: culturing 24-60 h under 4-28 DEG C. The fermenting culture medium contains in each liter: starch 10-20 g, ferrisulphas 0.01-0.05 g, potassium nitrate 0.8-1.2 g, dipotassium hydrogen phosphate 0.5-1 g, and magnesium sulfate 0.5-1 g. The pH value of the fermenting culture medium is 6-9. The purple bacteriocin from the bacterial strain is a natural product, and free from any hidden harm due to artificial pigment. Tests show that the purple bacteriocin is of good biologic activity, will generate no side effect on animal bodies, and can be widely used in such fields as medicine and industries, etc.

The invention provides Ochrobactrum guangzhouense P2 with high capacity in degrading pyridine. The strain as a novel species of Ochrobactrum genus is obtained by separating and purifying sludge of oil and gas factories, and is preserved in China Center for Type Culture Collection with the preservation number of CCTCC M 207191. The strain has higher capacity in degrading pyridine, and can effectively degrade pyridine in sludge, thus lowering the sludge processing cost.

The invention discloses a compound microorganism live bacteria preparation for enhancing activated sludge, a preparation method and applications thereof. The preparation contains Bacillus fusiformis, Comamonas sp. and Ochrobactrum sp.. The preparation method comprises the following steps: first, Bacillus fusiformis, Comamonas sp. and Ochrobactrum sp. after inclined plane activation are inoculated in liquid mediums respectively, and cultured at the temperature of 30 DEG C-37 DEG C for 40-54 h to obtain a microorganism bacteria liquid; second, the microorganism bacteria liquids obtained from the first step are mixed uniformly, dried at the temperature of 40 DEG C-60 DEG C, and the processes of crushing to 15-50 meshes and sieving are performed. The preparation can enhance activated sludge performances effectively and rapidly in applications as an activated sludge COD-degrading enhancer in the papermaking wastewater treatment process. The preparation can degrade papermaking wastewater COD efficiently and improve wastewater chromaticity and water quality.

The invention belongs to the field of biochemical engineering and processes, relates to a method for adsorbing metal ions by a biological adsorbent and in particular relates to an ochrobactrum intermedium extracellular polysaccharide and application of the ochrobactrum intermedium extracellular polysaccharide to the aspect of soil improvement. The method comprises the following steps: step (1): obtaining ochrobactrum intermedium capable of producing an extracellular polysaccharide; step (2): obtaining the ochrobactrum intermedium extracellular polysaccharide; step (3): adsorbing the metal ions through the ochrobactrum intermedium extracellular polysaccharide; step (4): desorbing the metal ions through the ochrobactrum intermedium extracellular polysaccharide.

The invention provides a Chinese herbal medicine feed additive. The Chinese herbal medicine feed additive is obtained by mixing components in traditional Chinese medicines of raw materials. The traditional Chinese medicines of raw materials comprises the following components in parts by mass: 15 parts of bark of Chinese corktree, 10 parts of stems of sargentgloryvine, 10 parts of dahurian patriniaherb, 10 parts of Chinese gall, 10 parts of pomegranate rind, 20 parts of spreading hedyotis herb, 15 parts of barbed skullcap herb, 15 parts of purslane, 25 parts of honeysuckles, 20 parts of Chinese pulsatilla roots, 20 parts of kudzuvine roots, 5 parts of coptis chinensis, 10 parts of atractylodes rhizomes, 20 parts of poria cocos, 5 parts of costusroot, 15 parts of medicated leaven, 10 partsof chicken's gizzard-membrane, 15 parts of bighead atractylodes rhizomes, 15 parts of hairy vein agrimonia herb, and 10 parts of Chinese mugwort leaves. The Chinese herbal medicine feed additive is used for feeding cultured fishes with enteritis, has the efficacy of regulating the structure of intestinal flora, and can obviously reduce the proportion and amount of harmful flora in intestinal tracts, improve the beneficial bacteria ratio of lactobacillales to bacillales, relieve the symptoms of the fishes with the enteritis, reduce the mortality of the fishes with the enteritis, and improve theacquiring rate of fishes.

The invention discloses an efficient phosphate solubilizing bacillus megaterium in a tree rhizosphere. The preservation number is CCTCC NO. M 2017454, and the efficient phosphate solubilizing bacillusmegaterium is named as Bacillus megaterium L205. The test shows that the phosphate solubilizing amount of bacillus megaterium reaches 203.04mg/L, and the liquid bacterium can effectively solubilize insoluble inorganic phosphorus, increase the content of available phosphorus in red-yellow soil, and promote the growth of tree seedlings. The efficient phosphate solubilizing bacillus megaterium provided by the invention enriches the sink population of bacillus megaterium at the culture collection center and provides convenience for better exploitation of microbial population resources of bacillusmegaterium. Meanwhile, the bacillus megaterium strain L205 can secrete organic acids, solubilize inorganic phosphorus and improve the phosphorus absorption and utilization of plants, is especially suitable for the production of tree biological fertilizers and tree growth promoting bacteria in red-yellow soil areas in South China, and can be used for further developing compound microbial fertilizers and bacteria.

The invention discloses an N-acyl high serine lactone quenching bacterium and an application thereof in disease control. Researches show that an ochrobactrum intermedium has quenching activity to various AHLs quorum sensing signal molecules, is separated to obtain an efficient quenching bacterium strain D-2, can degrade the various AHLs quorum sensing signal molecules quickly and remarkably, including OHHL, OOHL and OdDHL and the like, can alleviate AHLs dependent pathogenic bacteria disease symptoms remarkably and has a huge application potential in preventing and treating AHLs-mediated pathogenic bacterium harm. The strain is stable in activity, provides a novel development path of a treatment strategy for replacing chemical prevention with bioprevention and blocking quorum sensing as atarget without inducing selective pressure and has a huge popularizing and applying potential in preventing and treating quorum sensing mediated pathogenic bacteria harm. The anti-pathogenic biocontrol bacterium acting as quorum sensing has a wide application prospect.