Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Duganella bacterium and uses thereof

A technology of Duchenne bacterium and bacterial cells, which is applied in the direction of bacteria and fermentation, can solve the problems of limited number of species and few applied researches, and achieve the effects of fast growth, good application prospects, and convenient industrial production

Inactive Publication Date: 2008-03-12
TSINGHUA UNIV
View PDF0 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the number of known species of Duanella is limited, and its application is rarely studied

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Duganella bacterium and uses thereof
  • Duganella bacterium and uses thereof
  • Duganella bacterium and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1, the isolation and identification of Duganella sp. (Duganella sp.) B2 CGMCC №2056

[0029] 1. Sample collection

[0030] Soil samples were collected from Glacier No. 1 in Xinjiang.

[0031] 2. Isolation and screening of strains

[0032] Prepare separation plate medium: 5g of peptone, 3g of beef extract, 20g of agar, add 1000ml of water, pH7.0. The above medium was added at 1.05kg / cm 2 , 121°C, autoclaved for 20 minutes.

[0033] The soil sample was made into a 2% (2g / 100ml) suspension, and diluted to 2×10 by 10 times 8 , take 0.2mL sample solution of each dilution and spread evenly on the above-mentioned separation medium plate, culture at 25°C for 7 days, observe and find that there are blue single colonies, select the colonies, separate them by streaking, and pick them after culturing Get the bacterial single colony and carry out streak separation, repeat the above steps, until it is a pure bacterial strain (detected with a microscope, the cell shape...

Embodiment 2

[0047] Embodiment 2, utilize Duganella sp. (Duganella sp.) B2 CGMCC №2056 to produce violacein

[0048] 1. Strain culture

[0049] Prepare plate medium: 5g of peptone, 3g of beef extract, 20g of agar, add water to 1000ml, and adjust pH to 7.0. The plate medium was sterilized at 121° C. for 15 minutes, poured onto the plate, cooled and then inoculated with Duganella sp. B2 CGMCC №2056, and cultured at 25° C. for 72 hours.

[0050] Prepare seed liquid medium (content per liter): 5 g of peptone, 3 g of beef extract, and adjust pH to 7.0. Pick a single colony of Duganella sp. B2 CGMCC №2056 from the plate and inoculate it in a sterile liquid medium, and cultivate it at a temperature of 25° C. with a rotation speed of 200 rpm (rotation radius of 32 mm). After 24 hours of cultivation, the bacteria were in the logarithmic growth phase.

[0051] Preparation of fermentation liquid medium: starch 15g, ferrous sulfate 0.03g, potassium nitrate 1g, dipotassium hydrogen phosphate 0.7g, m...

Embodiment 3

[0086] Embodiment 3, utilize Duganella sp. (Duganella sp.) B2 CGMCC №2056 to produce violacein

[0087] 1. Culture of strains

[0088] Prepare plate medium: 5g of peptone, 3g of beef extract, 20g of agar, add water to 1000ml, and adjust pH to 7.0. The plate medium was sterilized at 121° C. for 15 minutes, poured onto the plate, cooled and then inoculated with Duganella sp. B2 CGMCC №2056, and cultured at 25° C. for 72 hours.

[0089]Prepare seed liquid medium (content per liter): 5 g of peptone, 3 g of beef extract, and adjust pH to 7.0. Pick a single colony of Duganella sp. B2 CGMCC №2056 from the plate and inoculate it in a sterile liquid medium, and cultivate it at a temperature of 25° C., with a stirring speed of 200 rpm (rotation radius of 32 mm). After 24 hours of cultivation, the bacteria were in the logarithmic growth phase.

[0090] Prepare fermentation broth: 10g starch, 0.01g ferrous sulfate, 0.8g potassium nitrate, 0.7g dipotassium hydrogen phosphate, 1g magnesi...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a Duganella bacterium and its application. The Duganella bacterium is sp. B2 CGMCC No:2056. Culturing Duganella sp. B2 CGMCC No:2056 will get purple bacteriocin. The fermenting condition for the bacterial strain is: culturing 24-60 h under 4-28 DEG C. The fermenting culture medium contains in each liter: starch 10-20 g, ferrisulphas 0.01-0.05 g, potassium nitrate 0.8-1.2 g, dipotassium hydrogen phosphate 0.5-1 g, and magnesium sulfate 0.5-1 g. The pH value of the fermenting culture medium is 6-9. The purple bacteriocin from the bacterial strain is a natural product, and free from any hidden harm due to artificial pigment. Tests show that the purple bacteriocin is of good biologic activity, will generate no side effect on animal bodies, and can be widely used in such fields as medicine and industries, etc.

Description

technical field [0001] The present invention relates to a strain of Duchenne and its application. Background technique [0002] Pigments are generally divided into synthetic pigments and natural pigments, both of which have been widely used in food colorants, daily cosmetics and feed additives. Synthetic pigments refer to organic pigments prepared by chemical synthesis methods, mainly extracted from coal tar or synthesized with aromatic compounds such as benzene, toluene, naphthalene, and aniline. After the appearance of artificial synthetic pigments, they were quickly accepted by users due to their bright color, strong coloring power, good stability, convenient color matching, and low price. However, with the development of modern medicine, food hygiene and other disciplines, people found that synthetic pigments Pigments have toxicological problems, some are harmful to human health, and even induce cancer. Therefore, more and more countries have stopped using some more to...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N1/20C12P1/04
Inventor 邢新会王海胜卢元薛园姜瑞波娄恺魏东
Owner TSINGHUA UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com