The invention discloses a short bacillus and an application thereof. The short bacillus is a curtobacterium sp. SY311 bacterial strain and is preserved in China General Microbiological Culture Collection Center (CGMCC) with the preservation number of CGMCC No.7181. The method for preparing trehalose by fermenting the short bacillus concretely comprises the following steps of: (1) activating the strain; (2) preparing a seed solution; (3) fermenting; (4) deproteinizing; (5) collecting a trehalose component; and (6) carrying out posttreatment, namely carrying out decoloration, filtering, low pressure concentration, crystallization and drying on the trehalose component, so that the trehalose product is obtained. According to the invention, the adopted strain is mutated by ultraviolet, trehalose enzymatic activity is low, and accumulative increase of trehalose in fermentation liquor can be facilitated, so that the yield of the final trehalose product is increased; and the preparation technology disclosed by the invention is simple, trehalose is directly synthesized by adopting a one-step method, raw materials are available, cost is low, less impurities are contained in the trehalose product, purification is easy, and the method provided by the invention is applicable to industrial production.