Method for culturing bacteria and application thereof

A technology of bacteria and nutrients, applied in the field of cultivating microorganisms, can solve problems such as complicated operation, high cost, and difficult to deal with harmful pollution

Inactive Publication Date: 2011-09-21
FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] Due to the shortcomings of traditional treatment methods, such as high cost, complicated operation, and difficult treatment of harmful pollution with large flow and low concentration, after years of exploration and research, biological treatment technology has been increasingly valued by people.

Method used

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  • Method for culturing bacteria and application thereof
  • Method for culturing bacteria and application thereof
  • Method for culturing bacteria and application thereof

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0019] Activation of example 1 bacterial classification

[0020] Take the preserved glycerol bacteria, insert it into a 100mL Erlenmeyer flask containing 50mL LB medium (pH7-8) at a ratio of 1:100, and place it on a 180r / min shaker under aerobic, normal pressure, and 30°C conditions. Shake culture in medium overnight to activate the strain.

example 2

[0021] Example 2 Nutrient condition optimization

[0022] Transfer the activated Bacillus human palei into four 250mL Erlenmeyer flasks containing 100mL of culture medium at a ratio of 1:100 between the bacteria liquid and the culture medium. As follows, No. 1 medium: tofu water + sugar residue extract (1%-10%); No. 2 medium: tofu water + glucose (1%-5%); No. 3: tofu water + sterile water; No. 4 Medium: tofu water + phosphate (0.01%-0.5%). Place the four Erlenmeyer flasks at 37°C and incubate them at 180r / min for 24h to measure the number of bacteria. Comparing the growth effect of Paleobacterium hominis in four different culture media, it was found that the growth of the bacteria was best in the tofu water added with sugar residue extract (1%-10%), and the growth condition was that No. 1 medium>4 Medium No. 3 > Medium No. 3 > Medium No. 2. see details figure 1 .

example 3

[0023] Example 3 Effects of different pH values ​​on the growth of human paleobacterium

[0024] According to Example 1, we selected the No. 1 medium with the best growth and reduction effect to carry out the following experiments. Get the bean curd water that has been subjected to acid hydrolysis, add sugar residue leaching solution (1%-10%), adjust the pH to be 4.5, 5.5, 6.5, 7.5, 8.5, 9.5 respectively, and insert the activated Pallidum pallidum with 1% inoculum, Divide into three groups in parallel, culture at 37°C, 180r / min for 24h, and measure the number of bacteria. The results show that within the range of pH=5.5-9.5, the growth of Pallidinus humanis in tofu water is better, and when the pH=7.5, the number of bacteria reaches the maximum value, so pH=7.5 is the best growth condition of Pallidinus humanis in tofu water Appropriate pH. see details figure 2 .

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Abstract

The invention provides a method for culturing bacteria and application thereof and relates to a method for culturing microorganisms with low price and application thereof to high-efficiency reduction and fixation of heavy metal. In the method for culturing the bacteria by using a low-price culture medium, bean curd water comprises rich carbon sources and nitrogen sources and low-price sugar dreg lixivium is added, human ochrobactrum lupini grows well when the pH value is 7.5, and escherichia coli, bacillus subtilis and bacillus cereus can grow under the conditions, so culture cost is greatly reduced. The human ochrobactrum lupini are further used for treating chromium-containing and nickel-containing industrial waste water in an optimized culture medium, and the result shows that the nickel and chromium reducing efficiency of the human ochrobactrum lupini is high.

Description

technical field [0001] The invention relates to a method for cultivating microorganisms and its application in efficient reduction and fixation of heavy metals. Background technique [0002] In my country, the yield of soybean is high, the planting area is wide, and there are many kinds of soybean products. Soybean is not only high in protein content, but also rich in calcium, phosphorus, potassium, iron, simple sugars, B vitamins, amino acids and organic acids and other substances that are beneficial to the human body. For the living habits and eating habits of our people, tofu products account for a large proportion of soybean food processing. Tofu is loved by people as a nutritious and economical traditional bean food. [0003] Survey data show that for every ton of tofu products produced, about one ton of "tofu water" is discharged, commonly known as "yellow pulp water". These "tofu water" are often directly discharged into the sewer, and the COD value in the wastewat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F1/58C12R1/01C12R1/085C12R1/125C12R1/19
Inventor 潘晓鸿林璋
Owner FUJIAN INST OF RES ON THE STRUCTURE OF MATTER CHINESE ACAD OF SCI
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