Method for producing biological medicament fertilizer product through solid state fermentation of biological residue

A technology of solid-state fermentation and biomedical fertilizer, applied in application, fertilization device, fertilizer mixture, etc., to achieve unique effects of regulating plant metabolism, low cost, and simple process

Active Publication Date: 2011-09-21
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, it has been reported that solid-state fermentation of wastewater sludge is used to produce Bt pesticides (K.Lachhab, R.D.Tyagi a, J.R.Vale'ro. Production of Bacillus thuringiensis biopesticides using wastewater sludge as a raw material: effect ofinoculum and sludge solids concentration.Process Biochemistry 37 (2001) 197-208), but there is no report to produce Bt biopesticide or biofertilizer by solid-state fermentation of biological residue

Method used

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  • Method for producing biological medicament fertilizer product through solid state fermentation of biological residue
  • Method for producing biological medicament fertilizer product through solid state fermentation of biological residue

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Experimental program
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Embodiment 1

[0035] Embodiment 1, take asparagus leftovers as raw material to prepare biological medicine fertilizer

[0036] (1) Pretreatment of raw materials: the asparagus scraps are crushed to 20 meshes, the initial water content of the asparagus scraps is adjusted to 65%, and the inorganic salt KH is added 2 PO 4 0.2%, MgSO 4 ·7H 2 O 0.1%, CaCO 3 1.0%, fully stir and mix, adjust the pH to 7.0, and after 20-30 minutes of moist heat sterilization, obtain sterile culture materials, cool to about 30°C for later use;

[0037] (2) Bacterial strain expansion culture: pick a ring of Bt bacterial lawn from the bacillus thuringiensis slant culture medium, inoculate in 100mL sterilized beef extract peptone liquid medium (beef extract 0.3%, peptone 1%, NaCl 0.2%) , pH7.0~7.2), carry out shaking flask culture, the culture conditions are: temperature 30 ℃, rotating speed 200 rpm, culture 7~8 hours, as Bt bacteria seed liquid;

[0038] (3) Solid-state fermentation: inoculate Bt seed liquid in...

Embodiment 2

[0040] Embodiment 2, take asparagus leftovers as raw material to prepare biological medicine fertilizer

[0041] (1) Pretreatment of raw materials: the asparagus scraps are crushed to 40 meshes, the initial water content of the asparagus scraps is adjusted to 70%, and KH 2 PO 4 0.075%, MgSO 4 ·7H 2 O 0.2%, CaCO 3 0.5% Stir and mix well, adjust the pH to 7.2, and after 20-30 minutes of damp heat sterilization, obtain sterile culture materials, cool to about 30°C for later use;

[0042] (2) Bacterial classification expansion culture: with embodiment 1;

[0043] (3) Solid-state fermentation: inoculate the Bt seed liquid into the aseptic culture material, stir and mix, and carry out solid-state fermentation. The fermentation conditions are: temperature 32°C, humidity 85%, ventilation rate 1: 2, during 11 hours of fermentation, Reduce the fermentation temperature to 27°C, and the fermentation time is 40 hours;

[0044] (4) Post-treatment of fermented materials: the fermente...

Embodiment 3

[0045] Embodiment 3, using asparagus residue as raw material to prepare biomedical fertilizer

[0046] (1) Pretreatment of raw materials: asparagus residues are crushed to 40 meshes, the initial water content of asparagus residues is adjusted to 75%, and inorganic salt KH 2 PO 4 0.2%; MgSO 4 ·7H 2 O 0.2%; MnSO 4 ·H 2 O 0.002%; FeSO 4 ·H 2 O0.002%; ZnSO 4 ·H 2 O 0.002%, CaCO 3 1.0%, fully stir and mix, adjust the pH to 7.4, and after 20-30 minutes of moist heat sterilization, obtain sterile culture materials, cool to about 30°C for later use;

[0047] (2) Bacterial classification expansion culture: with embodiment 1;

[0048] (3) Solid-state fermentation: Inoculate the Bt seed liquid into the aseptic culture material, stir and mix, and carry out solid-state fermentation. The fermentation conditions are: temperature 30°C, humidity 95%, ventilation rate 1: 1.2, in 10 hours of fermentation, Reduce the fermentation temperature to 26°C, and the fermentation time is 40 h...

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Abstract

The invention relates to the technical field of biological pesticides, in particular to a method for producing a biological medicament fertilizer product through solid state fermentation of biological residue. The method comprises the following steps of: 1) pretreating the biological residue; 2) performing solid state fermentation, namely inoculating bacillus thuringiensis seed liquor into a sterile culture material for solid state fermentation, wherein temperature-changing fermentation that the initial fermentation temperature is reduced by 2-5DEG C is adopted in the 8 to 11 hours of fermentation; and 3) adding chitosan oligosaccharide powder to obtain the biological medicament fertilizer product. The method has the advantages of low cost, simple process, low equipment investment, environment-friendly production process and the like; moreover, in the method, the chitosan oligosaccharide powder is added into a fermentation product, and the obtained biological medicament fertilizer has effects of killing insects in a broad spectrum and inhibiting viruses, has the functions of regulating plant metabolism and promoting plant growth, has the fertilizer efficiency integrating effect, and combines a biological pesticide and a biological fertilizer.

Description

technical field [0001] The invention relates to the technical field of biopesticides, in particular to a method for producing biomedicine fertilizer products by solid-state fermentation of biological residues. Background technique [0002] my country has joined the WTO, and agriculture will face new challenges and development opportunities. The problem of drug residues in agricultural by-products has attracted more and more attention from all countries. One of the important measures to improve the competitiveness of my country's agricultural products in the international market is to reduce the residual amount of toxic substances in agricultural products. Biological pesticides and biological fertilizers (referred to as biomedical fertilizers) will provide support for the high-quality and safe production of agricultural products and the reduction of toxic substance residues. Biomedical fertilizer is a new industry in the pesticide and fertilizer industry in the 21st century, r...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C05G3/02C05G3/60
Inventor 赵兵赵庆生王晓东袁晓凡
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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