Salmon and trout feed based on peroxisome proliferator activated receptor (PPAR) and method for preparing salmon and trout feed

A technology of fish feed and combined activity, applied in animal feed, animal feed, application, etc.

Inactive Publication Date: 2012-12-12
BEIJING FISHERIES RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the fish meat quality problem of the existing cultured fish, and proposes a fish feed b

Method used

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  • Salmon and trout feed based on peroxisome proliferator activated receptor (PPAR) and method for preparing salmon and trout feed

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0019] The present invention will be described in detail below through specific examples.

[0020] This example takes rainbow trout as the research object. First, determine the types of natural PPAR-binding active substances that can be added to fish feed, and then study the relationship between the feed and fish fat and fatty acid content after adding different amounts of natural PPAR-binding active substances. . The specific implementation process is as follows:

[0021] (1) The PPAR binding activity of natural substances can be tested by p-nitrophenol phosphate activity, and the specific steps are as follows:

[0022] First, the expression vector pGEX-4T of GST and rainbow trout PPARα or PPARβ receptor fusion protein, and the expression vector pET28a of transcription coactivator SRC1 and bacterial alkaline phosphatase BAP fusion protein, GST and PPAR receptor fusion protein and SRC1-BAP were constructed The fusion protein was obtained through the Escherichia coli in vitro...

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Abstract

The invention provides a salmon and trout feed based on a peroxisome proliferator activated receptor (PPAR) and a method for preparing the salmon and trout feed. The salmon and trout feed comprises proteins, fat, trace elements and other basic substances which are required by the salmon and trout growth, and further contains natural PPAR alpha and/or PPAR beta binding active substances, such as daidzin, curcumin, isoflavone, arachidonic acid, berberine, naringenin, polydatin, kaempferol, isoflavoues aglycone, conjugated linoleic acid and the like. The salmon and trout feed can regulate the fat and fatty acid anabolism of the cultured salmon and trout, the fat content of the salmon and trout is lowered, the beneficial omega-3 fatty acid content is improved, and the salmon and trout quality is improved.

Description

technical field [0001] The invention belongs to the technical field of aquaculture and aquatic feed production and processing, and specifically relates to a feed suitable for salmon and trout based on PPAR binding activity and a preparation method thereof, which can improve the quality of fish meat products of farmed salmon and trout. Background technique [0002] Fish meat is delicious, high in protein, and rich in unsaturated fatty acids that are beneficial to human health. It is an important food source for humans. Especially salmon and trout, its meat is tender, beautiful in color and rich in nutrition, especially the content of EPA and DHA is several times higher than that of other fish. Salmon, which is popular with consumers, is the trade name of certain salmon and trout species, and the commercially available Norwegian salmon is mainly Atlantic salmon. However, relying solely on catching wild fish can no longer meet people's needs, and the shortage of supply can onl...

Claims

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Application Information

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IPC IPC(8): A23K1/18A23K1/16A23K1/10
Inventor 贾成霞张清靖朱华刘盼曲疆奇杨慕
Owner BEIJING FISHERIES RES INST
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