Deodorant complex microbial inoculant and preparation method and application thereof

A technology of microbial deodorization and compound bacterial agent, which is applied in the field of microbial technology treatment to achieve the effects of reducing the use of antibiotics, lasting deodorizing effect, and improving the ecological environment of feces

Inactive Publication Date: 2016-01-20
北京市畜牧业环境监测站 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In recent years, most domestic researchers have focused on the screening of a single strain, but there are few reports on the application of microbial agents composed of microorganisms with different functions in practice.

Method used

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  • Deodorant complex microbial inoculant and preparation method and application thereof
  • Deodorant complex microbial inoculant and preparation method and application thereof
  • Deodorant complex microbial inoculant and preparation method and application thereof

Examples

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preparation example Construction

[0024] According to another aspect of the present invention, also provide a kind of preparation method of microbial deodorizing compound bacterial agent, comprise the steps: 1) solid slope culture: Bacillus megaterium and Bacillus subtilis are respectively inoculated on beef extract peptone solid medium In this method, mucormycetes and yeast were inoculated in potato solid medium, lactic acid bacteria were inoculated in MRS solid medium, and cultured at constant temperature to activate the strains. In a preferred embodiment of the present invention, the formula of beef extract peptone solid medium is: beef extract 5g, peptone 10g, NaCl 5g, distilled water 1000mL and solid plus agar 20g, pH value is 7.0-7.2. The formula of the potato solid medium is: 200g of potatoes, 20g of glucose, 1000mL of distilled water and 20g of solid plus agar, and the pH value is 7.0-7.2. The formula of MRS solid medium is peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, Tween 801.0mL, di...

experiment example 1

[0037] 1) Solid slant culture: inoculate LS-1 and LS-2 in beef extract peptone solid medium, J3 and J6 in potato solid medium, J2 in MRS solid medium, and culture at 30°C For 2 days, the strains were activated. Among them, lactic acid bacteria 4.8×10 8 cfu / mL; Mucor 2×10 6 cfu / mL; Yeast 3.9×10 7 cfu / mL; Bacillus megaterium 3×10 6 cfu / mL; Bacillus subtilis 7×10 6 cfu / mL; the total bacterial count is 5.31×10 8 cfu / mL. The formula of beef extract peptone solid medium is: beef extract 5g, peptone 10g, NaCl 5g, distilled water 1000mL, solid plus agar 20g, pH7.0-7.2. The formula of potato medium is: 200g potatoes, 20g glucose, 1000mL distilled water, 20g solid plus agar, pH7.0-7.2. The formula of MRS medium is peptone 10g, beef extract 10g, yeast extract 5g, glucose 20g, Tween 801.0mL, dipotassium hydrogen phosphate 2g, sodium acetate 5g, diammonium citrate 2g, magnesium sulfate heptahydrate 0.58g, sulfuric acid tetrahydrate Manganese 0.25g, distilled water 1000mL, pH6.2-6.6...

Embodiment 2-3

[0044] Its preparation method is the same as that of Example 1, the difference lies in the ratio of each strain, fermentation temperature, stirring rate, fermentation time, etc., see Table 1 for details.

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Abstract

The invention discloses a deodorant complex microbial inoculant and a preparation method and application thereof. The deodorant complex microbial inoculant is prepared from 4.8-10*10<8> cfu/mL of lactic acid bacteria, 2-5*10<6> cfu/mL of mucor, 3.9-7.2*10<7> cfu/mL of saccharomycetes, 3-10*10<6> cfu/mL of bacillus megatherium and 7-10*10<6> cfu/mL of bacillus subtilis, and the total count is 5.31-10.97*10<8> cfu/mL. The deodorant complex microbial inoculant is prepared from various microbial strains through fermentation, the deodorant complex microbial inoculant can grow well in feces due to the characteristics of flora composition, metabolic types, respiration types and functional diversity of effects, the deodorant complex microbial inoculant can grow well in feces and fast occupy favorable ecological niches, and therefore growth and propagation of putrefying bacteria producing repugnant substances can be effectively inhibited or the repugnant substances are directly degraded, repugnant odor in-situ source control is achieved, and the deodorization effect is achieved.

Description

technical field [0001] The invention relates to the field of microbial technology treatment, in particular to a microbial deodorizing composite bacterial agent and its preparation method and application. Background technique [0002] With the continuous development of the economy, the improvement of people's living standards and the continuous acceleration of the urbanization process, the problem of indoor and outdoor environmental pollution has become increasingly prominent. Urban and rural residents have higher and higher standards for the living environment and more and more attention to the requirements of air quality. As one of environmental hazards, stench directly affects people's quality of life and even endangers people's health, and has attracted more and more attention. Large-scale livestock and poultry farms are an important source of odorous substances. A large amount of livestock and poultry manure and domestic waste cannot be fully and effectively utilized, wh...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16C12N1/14B01D53/84C12R1/785C12R1/11C12R1/125C12R1/01
CPCY02A50/20Y02P20/59
Inventor 张加勇吴迪梅张丽丽张卓毅罗一鸣刘佳杨军任莉彭生平王有月刘薇
Owner 北京市畜牧业环境监测站
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