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Ochrobactrum anthropi FX02 strain and application thereof to wastewater denitrification

A technology of Paleobacterium hominis and strains, applied in the field of microbial denitrification, can solve the problems of low efficiency, discontinuous denitrification process, limited application and development, etc., and achieve good application prospects

Active Publication Date: 2017-08-18
JINAN UNIVERSITY
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  • Summary
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  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The traditional biological denitrification technology is that the two processes of nitrification and denitrification are independent of each other, resulting in discontinuity and low efficiency of the denitrification process, which greatly limits the application and development of traditional biological denitrification technology

Method used

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  • Ochrobactrum anthropi FX02 strain and application thereof to wastewater denitrification
  • Ochrobactrum anthropi FX02 strain and application thereof to wastewater denitrification
  • Ochrobactrum anthropi FX02 strain and application thereof to wastewater denitrification

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The specific steps for screening heterotrophic nitrification-aerobic denitrification strains are as follows:

[0028] 1. Domestication and enrichment: The experimental sludge comes from the aerobic activated sludge of Guangzhou Datansha Sewage Treatment Plant. Take 10g of the fully mixed activated sludge and place them in 500 mL of 200mL nitrification culture solution and denitrification culture solution. Place the Erlenmeyer flask in a shaker, set the temperature to 30°C, rotate speed to 150 r / min, and acclimate to a 3d cycle. After each cycle, let the culture medium in the bottle stand for a while, discard the supernatant, and re-add 200 mL of fresh culture medium for domestication. During the period, the removal of ammonia nitrogen and nitrate nitrogen was tested.

[0029] 2. Separation and purification: Dilute 1 mL of the acclimation solution to 10 with sterile water -1 , 10 -3 , 10 -5 10 -7 , And then spread on the heterotrophic nitrification and aerobic denitrificati...

Embodiment 2

[0035] Growth and denitrification characteristics of the FX02 strain described in Example 1

[0036] 1. After expanding the FX02 strain, resuspend the bacteria in sterile water (constant volume to 1g / L), and then inoculate 10% of the inoculum into a 250mL Erlenmeyer flask containing 100mL of heterotrophic nitrification medium. Shake well, incubate on a shaker at 30°C and 120r / min for 72 hours, and sample regularly to determine NH in the supernatant 4 + -N, NH 2 OH, NO 3 - -N, NO 2 - -N, COD, TN and OD 600 value.

[0037] See the result figure 2 ,according to figure 2 The results showed that when the FX02 strain uses ammonia nitrogen as the sole nitrogen source, the FX02 strain enters the logarithmic phase after 12 hours of growth, and the strain enters the decay phase after a short stable phase. While the strain is growing, NH 4 + -N, TN and COD decrease, NH 4 + -N is completely removed at 72 h, maximum NH 4 + -N removal rate reached 10.64 mg / L•h, and TN and COD removal rates rea...

Embodiment 3

[0042] Example 3 Application of the FX02 strain described in Example 1 for enhanced denitrification.

[0043] Water quality indexNH 4 + -N

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Abstract

The invention belongs to the technical field of microbial denitrogenation, and particularly discloses an ochrobactrum anthropi FX02 strain and application thereof to wastewater denitrification. The strain is preserved in Guangdong Microbiological Culture Collection Center (GDMCC) on August 17, 2016 with the preservation number of GDMCC No:60062. The strain is a heterotrophic nitrification-aerobic denitrification bacteria, nitrification and denitrification can be conducted simultaneously, and the strain has high application value in sewage treatment.

Description

Technical field [0001] The present invention relates to the technical field of microbial denitrification, in particular to a strain of Palebacterium anthracis FX02 and its application in wastewater denitrification. Background technique [0002] In view of the deteriorating water environment quality and the increasingly serious phenomenon of water eutrophication, the efficient and rapid removal of nitrogen in water is an important issue in the field of water pollution prevention and control. Traditional biological denitrification technology is independent of nitrification and denitrification, which leads to the discontinuity and low efficiency of the denitrification process, which greatly limits the application and development of traditional biological denitrification technology. At present, the development and application of some new biological denitrification technologies provide new ideas for biological denitrification. Many new denitrification bacteria have been discovered one...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/01
CPCC02F3/34C12N1/205C12R2001/01
Inventor 曹刚李紫惠黄郑郑陈海升莫测辉
Owner JINAN UNIVERSITY
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