33 results about "Ochrobactrum anthropi" patented technology

The invention establishes aminopherase through genetic engineering. Compared with ochrobactrum anthropi based wild w-aminopherase coming from ochrobactrum anthropi, the enzyme activity of aminopherase is remarkably improved, and aminopherase can be used for industrially producing L-2-aminobutyric acid.

The present invention discloses one kind of human Ochrobactrum anthropi IBL01 (Ochrobactrum anthropi CCTCC M 206046) and its application in degrading plant stalks. Ochrobactrum anthropi IBL01 has high plant stalk degrading effect and can produce high activity cellulase, hadromse and hemicellulase. The present invention also discloses the preparation process of cellulose, xylanase, lignin peroxidase, laccase and manganese peroxidase with the human Ochrobactrum anthropi. The human Ochrobactrum anthropi of the present invention is bacterium with short growth period of about one week, and can produce fiber degrading enzyme as well as hemicellulose degrading enzyme and lignin degrading enzyme.

The invention discloses a compound microbial active filling material for removing sulphur-containing repugnant substances, as well as preparation and application of the compound microbial active filling material. Ochrobactrum anthropi SL1 and aquamicrobium defluvii SU1 related in the invention are isolated bacteria obtained in a reactor for processing repugnant substances, such as H2S, in a water pollution control laboratory of the ecological environment research centre of the Chinese academy of sciences. The bacterial strains are preserved in the general microbial centre of the Chinese general microbial strain preservation and management committee. The preservation numbers are CGMCC No.7400 and CGMCC No.7399 respectively. The compound microbial active filling material disclosed by the invention has the advantages of being large in specific area, high in porosity, good in air permeability, low in resistance, high in bacterial cell loading capacity and less prone to run away; and because of being loaded with ochrobactrum anthropi SL1 and aquamicrobium defluvii SU1, the compound microbial active filling material is difficult to decay and deform in a bioreactor for purifying waste gas, and therefore, the compound microbial active filling material can be used for a long time and is applied to complex processing conditions.

The invention relates to a composite microbial preparation of linear alkyl benzene sulphonic acid as well as a preparation method and application thereof. The composite microbial preparation of the linear alkyl benzene sulphonic acid contains Bacillus subtilis, Ochrobactrum anthropi and pseudomonas aeruginosa; the microbial content of the Bacillus subtilis accounts for 15-35% that of the total microbial content; the microbial content of the Ochrobactrum anthropi accounts for 40-60% that of the total microbial content; and the microbial content of the pseudomonas aeruginosa accounts for 10-30% that of the total microbial content. The composite microbial preparation has the advantages that the linear alkyl benzene sulphonic acid can be rapidly decomposed and thoroughly oxidized into CO2 and H2O, so that the growth of harmful microbe is effectively suppressed, the biological treatment efficiency of LAS (Sodium Dodecyl Benzene Sulfonate) wastewater is greatly increased, and the odor of sewage is eliminated.

The invention discloses ochrobactrum anthropi SL1 and application thereof to removal of repugnant substances containing sulfur. Ochrobactrum anthropi SL1 is an isolate obtained from a reactor for treating repugnant substances containing H2S and the like in a water pollution control laboratory in Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences. The strain is collected in China General Microbiological Culture Collection Center (CGMCC), with collection number of CGMCC No.7400. The strain can be used for preparing microbial active fillers, is used for purifying the repugnant substances containing sulfur and solving the problem of odor pollution produced in the processes of sewage, sludge and refuse treatment and composting, and has more than 90% of efficiency of degrading repugnant substances containing sulfur such as hydrogen sulfide, methanethiol, dimethyl sulfide and the like.

The invention provides a method for culturing bacteria and application thereof and relates to a method for culturing microorganisms with low price and application thereof to high-efficiency reduction and fixation of heavy metal. In the method for culturing the bacteria by using a low-price culture medium, bean curd water comprises rich carbon sources and nitrogen sources and low-price sugar dreg lixivium is added, human ochrobactrum lupini grows well when the pH value is 7.5, and escherichia coli, bacillus subtilis and bacillus cereus can grow under the conditions, so culture cost is greatly reduced. The human ochrobactrum lupini are further used for treating chromium-containing and nickel-containing industrial waste water in an optimized culture medium, and the result shows that the nickel and chromium reducing efficiency of the human ochrobactrum lupini is high.

The invention discloses a 5-enolpyruvyl-shikimate-3-phosphate (EPSP) synthase gene from ochrobactrum anthropi and application thereof. The invention also provides genetic engineering intermediates (such as an expression cassette, a carrier and a cell) of the gene, a method for obtaining a plant with glyphosate resistance, and application of the method, and provides an identification method for judging whether the plant has the glyphosate resistance. The EPSP synthase gene plays an important role in research and development of the glyphosate-resistant plant.

The invention discloses an azoxystrobin degrading bacterium and a microbial inoculum produced by using the degrading bacterium and applications of the degrading bacterium. The strain is ochrobactrum anthropi (Ochrobactrum (anthropi) SH14, the strain is preserved in China Center For Type Culture Collection on December 20, 2013, and the Preservation Number is CCTCC NO: M 2013681. The strain can effectively degrade azoxystrobin pesticide residues in a short time, so that the strain can be used for restoring natural environments such as water and soil and the like polluted by azoxystrobin. A preparation prepared by using the strain has the advantages of low production cost, easiness for use, good removal effect, and the like. By using the microbial inoculum, the residual quantity of azoxystrobin in water and soil can be reduced by over 85% in a short period of time, so that natural environments polluted by azoxystrobin are effectively restored, and the excessive pesticide residue problem and the environmental pollution problem in agricultural production are solved, thereby protecting the ecological environment and the human health.

The invention discloses a microbial inoculum for degrading straws at low temperature and a preparation method thereof. The microbial inoculum disclosed by the invention is prepared from ochrobactrum anthropi, lindernia procumbens, variovorax and nitrogen-producing pseudomonas. The bacterial strains comprise the following components in parts by weight: 30 to 50 parts of ochrobactrum anthropi, 10 to 25 parts of lindernia procumbens, 20 to 30 parts of variovorax and 25 to 40 parts of nitrogenous pseudomonas. The microorganisms are all screened from a low-temperature environment, various microorganisms have strong degradation ability to cellulose, and the microbial agent obtained by compounding the microorganisms can improve the degradation rate of straw at low temperature and effectively promote straw decomposition. The application of the fertilizer can promote straw returning to the field, improve soil fertility and reduce environmental pollution caused by random stacking and rotting of the straw.

The invention belongs to the technical field of microbial agents, and particularly relates to ochrobactrum anthropic with hexavalent chromium reduction and active black 5 degradation functions and anapplication of the ochrobactrum anthropic. The ochrobactrum anthropic is ochrobactrum anthropic S1 and is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2020461. The ochrobactrum anthropic disclosed by the invention can perform hexavalent chromium reduction and active black 5 degradation at the same time, and has good treatment prospects on Cr pollution and active black 5 pollution.

The invention belongs to the technical field of waterproof materials, particularly relates to a water-awakening type self-healing microbial capsule, and further relates to a waterproof material prepared from the microbial capsule. The microbial capsule is prepared from the following raw materials: ochrobactrum, a culture medium, nutrient substances, a calcium source, urea, a mixture of aluminate and gypsum, and swelling resin. The waterproof material provided by the invention can realize secondary repair, and can be directly awakened when meeting water, when a concrete member cracks, water flows into a crack, and the aluminate and the gypsum finish the first repair. And when the expanded crystal repaired for the first time cracks, water is in contact with the ochrobactrum anthropi along with the cracks, the ochrobactrum anthropi has a mineralization deposition effect, and the cracks are filled with mineralization deposition products again, so that the second time of repairing is completed. The microbial capsule and the waterproof material provided by the invention have the advantages of low cost of adopted materials, easily available raw materials, simple product production process and remarkable social and economic effects.