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Ochrobactrum anthropic with hexavalent chromium reduction and active black 5 degradation functions and application of ochrobactrum anthropic

A technology of Bacillus pallidum and Bacillus pallidum, applied in bacteria, chemical instruments and methods, microorganism-based methods, etc., can solve problems such as reduced efficiency, and achieve the effect of huge practical application prospects.

Inactive Publication Date: 2021-04-02
CENT SOUTH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, metals such as copper and chromium are often used to fix the color of dyes, so chromates containing hexavalent chromium (Cr(Ⅵ)) often coexist with dyes in textile, dyeing, and leather wastewater, and dyes and heavy metal pollutants themselves are both It is toxic and has an inhibitory and lethal effect on microorganisms. Therefore, the efficiency of microorganisms in treating wastewater containing active black 5 and Cr(Ⅵ) will be greatly reduced
At present, physical methods, chemical methods, biological methods and the combined application of different methods for the treatment of wastewater containing active black 5 or Cr(Ⅵ) have been relatively common, but microorganisms that can treat active black 5 and Cr(Ⅵ) pollution are not rarely reported

Method used

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  • Ochrobactrum anthropic with hexavalent chromium reduction and active black 5 degradation functions and application of ochrobactrum anthropic
  • Ochrobactrum anthropic with hexavalent chromium reduction and active black 5 degradation functions and application of ochrobactrum anthropic
  • Ochrobactrum anthropic with hexavalent chromium reduction and active black 5 degradation functions and application of ochrobactrum anthropic

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Effect test

Embodiment 1

[0021] Embodiment 1: Morphological characteristics of degrading active black 5 and reducing hexavalent chromium strain Ochrobactrum anthropic S1 of the present invention

[0022] Streak inoculate a single colony into LB solid medium, place the plate upside down in a constant temperature incubator, and incubate at 30°C for 12h. Gram-negative, obligate aerobic, the colonies are smooth and round, with a raised center and bright translucent.

Embodiment 2

[0023] Example 2: Screening and Phylogenetic Identification of Degradative Active Black 5 and Hexavalent Chromium-reducing Strain Ochrobactrum anthropic S1 of the Present Invention

[0024] (1) Activated sludge samples collected by Guangzhou Dongguan Textile Printing and Dyeing Factory were sent to the laboratory at low temperature for further screening and analysis of the target strains.

[0025] (2) In the laboratory environment: utilize the anaerobic reactor to carry out further domestication to the activated sludge, the stoste after domestication carries out gradient dilution, with 10 -6 In order to dilute the final concentration, spread evenly on LB solid plate, and incubate in a 30°C constant temperature incubator for 12 hours. After repeated separation and purification, a single strain S1 capable of degrading active black 5 and reducing hexavalent chromium was finally obtained.

[0026] (3) The genomic DNA of S1 was extracted and its 16S rDNA fragment was amplified, an...

Embodiment 3

[0027] Embodiment 3: Degradation active black 5 of the present invention and reduction hexavalent chromium bacterial strain Ochrobactrum anthropic S1 are to the removal effect of active black 5 and hexavalent chromium

[0028] (1) Prepare LB liquid medium, and pick a single colony of Ochrobactrum anthropic S1 on the LB solid plate to inoculate in the LB liquid medium, and cultivate overnight, which is the S1 seed solution.

[0029] (2) Mix 10mL seed liquid with 90mL sterilized medium, then add potassium dichromate powder dried to constant weight in this medium, so that the Cr(Ⅵ) concentration in the system is 20mg / L, and Cultivate at 30°C, 180rpm. Samples were taken every 12 hours to analyze the remaining Cr(Ⅵ) content. The results showed that Ochrobactrum anthropic S1 had a removal rate of 80.6% for Cr(Ⅵ).

[0030](3) Mix 10mL seed liquid with 90mL sterilized medium, then add reactive black 5 powder to this medium to make the concentration 50mg / L, and cultivate it under sta...

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Abstract

The invention belongs to the technical field of microbial agents, and particularly relates to ochrobactrum anthropic with hexavalent chromium reduction and active black 5 degradation functions and anapplication of the ochrobactrum anthropic. The ochrobactrum anthropic is ochrobactrum anthropic S1 and is preserved in China Center for Type Culture Collection, and the preservation number is CCTCC NO: M 2020461. The ochrobactrum anthropic disclosed by the invention can perform hexavalent chromium reduction and active black 5 degradation at the same time, and has good treatment prospects on Cr pollution and active black 5 pollution.

Description

technical field [0001] The invention belongs to the technical field of microbial inoculum agents, and in particular relates to human palebacterium pallidus capable of reducing hexavalent chromium and degrading active black 5 and its application. Background technique [0002] my country is one of the first countries in the world to use dyes, and it is also the country with the largest production, consumption and trade of dyes in the world. Dyes have a wide range of applications and are commonly used in many industries such as textiles, printing and dyeing, leather and papermaking. In the process of industrial application, the utilization rate of dyestuffs is low, and about 15% of the dyestuffs can not be utilized and are discharged into the environment. According to reports, the annual wastewater discharge of my country's textile industry alone is as high as 1.84 billion tons, and about 2000-3000 tons of dyes enter the environment with wastewater every year. Dyes containing...

Claims

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Application Information

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IPC IPC(8): C12N1/20C02F3/34C02F101/22C02F101/10C12R1/01
CPCC12N1/20C02F3/34C02F2101/22C02F2101/10
Inventor 周洪波王玉光袁明珠程海娜
Owner CENT SOUTH UNIV
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