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Composite microbial preparation of linear alkyl benzene sulphonic acid as well as preparation method and application thereof

A technology of sodium linear alkylbenzene sulfonate and compound microorganisms, applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of incomplete degradation and inefficient degradation, and achieve the suppression of harmful microorganisms Effects of growth, elimination of odor, and inhibition of reproduction

Inactive Publication Date: 2013-01-30
ANHUI AGRICULTURAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to provide a high-efficiency degradation direct A compound microbial preparation of sodium alkylbenzene sulfonate (LAS) and its preparation method and application, the compound microbial preparation can rapidly decompose sodium linear alkylbenzene sulfonate and completely oxidize it into CO 2 and H 2 O, effectively inhibit the growth of harmful microorganisms, greatly improve the efficiency of biological treatment of wastewater containing LAS, and eliminate the odor of sewage

Method used

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  • Composite microbial preparation of linear alkyl benzene sulphonic acid as well as preparation method and application thereof
  • Composite microbial preparation of linear alkyl benzene sulphonic acid as well as preparation method and application thereof
  • Composite microbial preparation of linear alkyl benzene sulphonic acid as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Step 1: Carry out amplified culture separately

[0041] After Bacillus subtilis CICC 20682 is activated on a slant, it is inoculated in a liquid medium and cultured at 30°C for 48 hours. The liquid medium formula is 1.5% soybean powder, 1% starch, 0.2% yeast extract, 1% glucose and the balance is water , pH 6.5.

[0042] Paleobacterium humanis CCAM 030159 was activated on a slant, inoculated in a liquid medium, and cultured at 30°C for 48 hours. The liquid medium formula was glucose 0.02%, KH 2 PO 4 0.12%, NH 4 Cl 0.04%, MgCl 2 ·6H 2 O 0.02%, ferric citrate 0.01% and the rest are composed of water, pH is 7.0.

[0043] Pseudomonas aeruginosa CICC 20546 was activated on a slope, inoculated in a liquid medium, and cultured at 30°C for 72 hours. The liquid medium was formulated with 1% beef extract, 1% peptone, 1% glucose, 0.5% sodium chloride and other The quantity is composed of water and has a pH of 7.0.

[0044] Viable bacteria count, the number of detected bact...

Embodiment 2

[0048] Step 1: Carry out amplified culture separately

[0049] Bacillus subtilis CICC 20682 and CGMCC 1.385 were activated on a slant, inoculated in a liquid medium, and cultured at 35° C. for 50 hours. The formulation of the liquid medium was the same as in Example 1.

[0050] Paleobacterium humanum CCAM 030 159, CICC 22020, and CICC 21952 were activated on a slant, inoculated in a liquid medium, and cultured at 32° C. for 50 hours. The formulation of the liquid medium was the same as in Example 1.

[0051] Pseudomonas aeruginosa CICC 20546 and CGMCC 1.860 were activated on a slant, inoculated in a liquid medium, and cultured at 28° C. for 96 hours. The formulation of the liquid medium was the same as in Example 1.

[0052] Viable bacteria count, the number of detected bacteria, mixed according to the percentage of the number of the following bacteria in the total number of bacteria: 30% of Bacillus subtilis, 55% of Pseudomonas aeruginosa, 15% of the total number of viable ba...

Embodiment 3

[0056] Step 1: Carry out amplified culture separately

[0057] After the Bacillus subtilis CGMCC 1.385 was activated on a slant, it was inoculated in a liquid medium and cultured at 32° C. for 48 hours. The formulation of the liquid medium was the same as in Example 1.

[0058] Paleobacterium humanis CICC 22020 and CICC 21952 were activated on a slope, inoculated in a liquid medium, and cultured at 30° C. for 48 hours. The formulation of the liquid medium was the same as in Example 1.

[0059] Pseudomonas aeruginosa CICC 20546 was activated on a slant, inoculated in a liquid medium, and cultured at 30° C. for 72 hours. The formulation of the liquid medium was the same as in Example 1.

[0060] Viable bacteria count, the number of detected bacteria, mixed according to the percentage of the following bacteria in the total number of bacteria: Bacillus subtilis 30%, Pseudomonas aeruginosa 20%, the total number of viable bacteria is high At 1.5 billion / ml fermentation broth sample...

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Abstract

The invention relates to a composite microbial preparation of linear alkyl benzene sulphonic acid as well as a preparation method and application thereof. The composite microbial preparation of the linear alkyl benzene sulphonic acid contains Bacillus subtilis, Ochrobactrum anthropi and pseudomonas aeruginosa; the microbial content of the Bacillus subtilis accounts for 15-35% that of the total microbial content; the microbial content of the Ochrobactrum anthropi accounts for 40-60% that of the total microbial content; and the microbial content of the pseudomonas aeruginosa accounts for 10-30% that of the total microbial content. The composite microbial preparation has the advantages that the linear alkyl benzene sulphonic acid can be rapidly decomposed and thoroughly oxidized into CO2 and H2O, so that the growth of harmful microbe is effectively suppressed, the biological treatment efficiency of LAS (Sodium Dodecyl Benzene Sulfonate) wastewater is greatly increased, and the odor of sewage is eliminated.

Description

technical field [0001] The invention belongs to the field of microbial environmental protection, and in particular relates to a compound microbial preparation for efficiently degrading linear alkylbenzene sulfonate (LAS) and its preparation method and application. Background technique [0002] Sodium linear alkylbenzene sulfonate (Linear Alkylbenzene Sulphonates, LAS) is a class of anionic surfactants with good decontamination, emulsification, solubilization and penetration capabilities. It is widely used in various fields of industrial and agricultural production and people's daily life. Increasing day by day, its seriously polluted water body, soil and serious pollution to organisms and environment have received people's attention. At present, most of the synthetic detergents produced on the market are anionic surfactants, mainly linear alkylbenzene sulfonate (LAS), whose output accounts for about 90% of the total output of synthetic detergents. LAS generally enters water...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C02F3/34C12R1/125C12R1/385C12R1/01C02F101/34
Inventor 赵海泉李南华曾苏胡子全李春燕沈超
Owner ANHUI AGRICULTURAL UNIVERSITY
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