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A strain of * degrading bacteria and its application

A technology of strains and strains, applied in the field of biodegradation treatment of contaminated soil and water, can solve the problems of restricting development and application, lack of efficient polycyclic aromatic hydrocarbon degrading bacteria, etc.

Inactive Publication Date: 2018-10-26
BEIJING NORMAL UNIVERSITY
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0003]However, due to the lack of efficient PAH-degrading bacteria, the further development and application of PAH-contaminated soil bioremediation technology is restricted

Method used

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  • A strain of * degrading bacteria and its application

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Embodiment Construction

[0019] to join Oil-contaminated soil was added to the open glass bottle of basal medium Enrichment and acclimatization of degrading microorganisms, added after 30 days The conical flasks of basal culture medium, trace metal solution, vitamin c solution and adsorbent are inserted into enriched and domesticated soil. After culturing in a shaker at a temperature of 25-30°C and a rotation speed of 100r / min for 5-7 days, the cycle transfer is carried out, and it can be efficiently removed after the number of cycles is greater than 8 times. of degrading bacteria. Made in a clean bench The bottom plate of agar solid medium as the only carbon source and energy source, on the bottom plate, make a plate containing Degrade the agar solid medium top plate of the bacterial population, and culture the prepared medium plate in an incubator for 10 to 15 days. In the ultra-clean bench to join , basal medium, trace metal liquid, vitamin c solution and adsorbent conical flasks were ...

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Abstract

The invention discloses a bacterial strain with degradative function belonging to the technical field of biodegradation treatment. The degrading bacterium was identified as Ochrobactrum anthropi DW1, and the strain preservation number was CGMCC No.8621. The bacterial strain was made into a bacterial suspension with a bacterial content of 1.25×108CFU / ml, and the degradation removal rate of the initial concentration of 0.14mg / L after 7 days was 56.2%.

Description

technical field [0001] The invention belongs to the technical field of biodegradation treatment of polluted soil and water, in particular to a plant degrading bacteria. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) are a class of condensed ring compounds containing two or more benzene rings arranged in linear, angular or clustered shapes, with hydrophobicity, low solubility, low vapor pressure, High melting point and boiling point, high octanol-water partition coefficient and other characteristics. Polycyclic aromatic hydrocarbons have strong carcinogenic, teratogenic and mutagenic effects. Generally speaking, low-molecular-weight PAHs with 2-3 rings have significant acute toxicity, while some high-molecular-weight PAHs have potential carcinogenicity. Due to the presence of conjugated large π-bond electron systems in PAHs molecules, they have high stability, their photolysis and biodegradation are slow, and they ca...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20B09C1/10C12R1/01
CPCB09C1/10C12N1/205C12R2001/01
Inventor 朱宜秦伟谢恩郑蕾丁爱中豆俊峰
Owner BEIJING NORMAL UNIVERSITY
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