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Microbial inoculum for degrading straw at low temperature and preparation method thereof

A technology for inoculum and straw, applied in the field of agricultural microorganisms, can solve the problems of restricting soil microorganisms, affecting the efficiency of straw returning to the field, reducing the degradation rate of corn straw, and achieving the effects of improving soil fertility, promoting the returning of straw to the field, and promoting the decomposition of straw.

Inactive Publication Date: 2021-11-16
SHENYANG AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Northeast is a cool area, with an average annual low temperature of -10.7°C to 9.65°C. The low ground temperature limits the activities of soil microorganisms, greatly reduces the degradation rate of corn straw, and affects the efficiency of straw returning to the field.

Method used

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  • Microbial inoculum for degrading straw at low temperature and preparation method thereof
  • Microbial inoculum for degrading straw at low temperature and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0030] The bacterial agent for degrading straw in this embodiment is composed of 3.0 kg of Pseudomonas bacterium liquid, 2.5 kg of Pomobacterium bacterium liquid, 2.7 kg of phagocytosis bacterium liquid and 3.5 kg of Pseudomonas azogenes bacterium liquid.

[0031] The bacterial agent is prepared through the following steps.

[0032]Step 1, activating the bacterial strain and preparing liquid seed solution. The inventors firstly activated the preserved strains of Paleobacterium pallidum, Phytophthora phagocytosis, Phytophagocystis and Pseudomonas azogenes using CMC-Na Congo red medium, and then transferred the activated strains into liquid enzyme-producing culture The base was cultured statically at 15-20°C for 3 days to obtain a liquid seed liquid of a single strain. The specific formula of CMC-Na Congo red medium is: sodium carboxymethylcellulose (CMC-Na) 10.0g, potassium nitrate (KNO 3 ) 1.0g, dipotassium hydrogen phosphate (K 2 HPO 4 ) 0.5g, magnesium sulfate (MgSO 4 ....

Embodiment 2

[0036] The bacterial agent for degrading straw in this embodiment is composed of 3.5 kg of Pallidum bacterium liquid, 2.0 kg of Pomo bacterium liquid, 2.3 kg of phagocytosis bacterium liquid and 2.5 kg of Pseudomonas azogenes bacterium liquid by weight.

[0037] The preparation process of the bacterial agent is similar to Example 1. The difference is that the fermentation temperature in step 2 is 10-15°C, and the static culture time is 6 days.

Embodiment 3

[0039] The bacterial agent for degrading straw in this embodiment is composed of 4.5 kg of Pallidum bacterium liquid, 1.5 kg of Pomo bacterium liquid, 2.0 kg of phagocytosis bacterium liquid and 4.0 kg of Pseudomonas azogenes bacterium liquid by weight.

[0040] The preparation process of the bacterial agent is similar to Example 1. The difference is that the fermentation temperature in step 2 is 15-20°C, and the static culture time is 5 days.

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Abstract

The invention discloses a microbial inoculum for degrading straws at low temperature and a preparation method thereof. The microbial inoculum disclosed by the invention is prepared from ochrobactrum anthropi, lindernia procumbens, variovorax and nitrogen-producing pseudomonas. The bacterial strains comprise the following components in parts by weight: 30 to 50 parts of ochrobactrum anthropi, 10 to 25 parts of lindernia procumbens, 20 to 30 parts of variovorax and 25 to 40 parts of nitrogenous pseudomonas. The microorganisms are all screened from a low-temperature environment, various microorganisms have strong degradation ability to cellulose, and the microbial agent obtained by compounding the microorganisms can improve the degradation rate of straw at low temperature and effectively promote straw decomposition. The application of the fertilizer can promote straw returning to the field, improve soil fertility and reduce environmental pollution caused by random stacking and rotting of the straw.

Description

technical field [0001] The invention belongs to the technical field of agricultural microorganisms, and in particular relates to a bacterial agent for degrading straw at low temperature and a preparation method thereof. Background technique [0002] Corn is one of the important food crops in my country, and it is also the basic raw material of many chemical products. With the increasing market demand for corn, the planting area of ​​corn is gradually expanding. But at the same time, the amount of corn stalks is also increasing. On the premise of not polluting the environment, how to effectively degrade corn stalks and return them to the field has become a difficult problem for corn planting areas. [0003] Northeast China is the largest corn planting area in my country, and the perennial corn planting area and total output account for about 35% of the country's total. The Northeast region is a cool area, with an annual average low temperature of -10.7°C to 9.65°C. The low ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C05F17/20C12R1/38C12R1/01
CPCC12N1/20C05F17/20Y02W30/40
Inventor 韩梅罗培宇黄玉茜卫季辉董珊珊王靖然李超敏魏冉彭帅刘艳何志刚侯雨昕杨丽娟
Owner SHENYANG AGRI UNIV
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