Microbial agent used for degrading quinclorac herbicides
A microbial inoculum, a technology for quinclorac, which is applied in the agricultural field, can solve the problems of non-degradation, deformity, yield reduction and the like, and achieves the effect of degrading the content of quinclorac
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Embodiment 1
[0014] A microbial bacterial agent for degrading quinclorac herbicides, in parts by weight, comprising: Bordetella 1.8g, Arthrobacter 1.8g, Burkholderia cepacia 1.3g, and Bacillus pallidum 1g , Pseudomonas 1.6g, Aspergillus niger 1.2g, Neurospora 1.3g, and the above-mentioned strains are all commercially available. The fermentation broths obtained after the above-mentioned strains were fermented and cultivated were mixed to form a mixed fermentation broth, 237.6 g of thalline cells were collected after centrifugation of the mixed fermentation broth, 23.76 g of skim milk and 28.5 g of sucrose were added, and then freeze-dried and mixed with 12g of cellulase, 24g of amylase, 26g of protease, 26g of lipase and 28g of bioaccelerator are compounded to obtain microbial bacterial agent.
[0015] Among them, Bordetella was inoculated in LB medium for fermentation, the culture temperature was 34°C, and cultured in a constant temperature shaking box with a rotation speed of 150r / min for...
Embodiment 2
[0023] A microbial agent for degrading quinclorac herbicides, in parts by weight, comprising: Bordetella 1.5g, Arthrobacter 2.1g, Bacillus megaterium 1g, Burkholderia cepacia 1.5 g, 1.6g of Pseudomonas, 1g of Aspergillus niger, 1.3g of Neurospora, all of which are commercially available. Mix the fermented liquids obtained after the fermentation and cultivation of the above strains respectively to form a mixed fermented liquid, collect 276.31 g of bacterial cells after centrifuging the mixed fermented liquid, add 27.6 g of skim milk and 33.16 g of sucrose, and then freeze-dry Compound with 28g of cellulase, 46g of amylase, 64g of protease, 38g of lipase and 57g of bioaccelerator to obtain microbial agent.
[0024] Among them, Bordetella was inoculated in LB medium for fermentation, the culture temperature was 34°C, and cultured in a constant temperature shaking box with a rotation speed of 130r / min for 18h;
[0025] Arthrobacter was inoculated in the culture formula as glucose...
Embodiment 3
[0032] A microbial agent for degrading quinclorac herbicides, in parts by weight, comprising: Bordetella 2g, Arthrobacter 1.8g, Bacillus megaterium 0.3g, Burkholderia cepacia 1.7 g, 1.5 g of Alcaligenes faecalis, 1.3 g of Aspergillus niger, and 1.4 g of Neurospora, all of which are commercially available. Mix the fermented liquids obtained after the fermentation and cultivation of the above-mentioned strains respectively to form a mixed fermented liquid, collect 264.21 g of bacterial cells after centrifuging the mixed fermented liquid, add 26.42 g of skim milk and 31.7 g of sucrose, and then freeze-dry Compound with 32g of cellulase, 34g of amylase, 34g of protease, 42g of lipase and 44g of bioaccelerator to obtain microbial bacterial agent.
[0033] Among them, Bordetella was inoculated in LB medium for fermentation, the culture temperature was 34°C, and cultured in a constant temperature shaking box with a rotation speed of 150r / min for 18h;
[0034] Arthrobacter was inocul...
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