Microbial agent used for degrading quinclorac herbicides

A microbial inoculum, a technology for quinclorac, which is applied in the agricultural field, can solve the problems of non-degradation, deformity, yield reduction and the like, and achieves the effect of degrading the content of quinclorac

Inactive Publication Date: 2020-09-25
SHANDONG SUNWAY LANDSCAPE TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] Quinclorac is a highly selective hormone-type herbicide, which has the advantages of being safe to rice and having special effects on barnyard grass. It is widely used in agricultural production, but the residual time of quinclorac herbicides is relatively long and it is not easy to degrade. easy to remain in the soil
It has been reported that quinclorac herbicides can remain in the soil for 2-3 years, even up to 4 years, and it is easy to cause phytotoxicity to subsequent crops in continuous cropping or rotation crops, especially some vegetables such as umbels. Sensitive crops such as Solanaceae and Solanaceae economic crops will produce phytotoxicity, leading to yield reduction, serious or even failure
In addition, quinclorac can also be enriched in agricultural products, causing problems such as food safety
Studies have shown that long-term consumption of agricultural products contaminated by organochlorine pesticides will lead to the accumulation of pesticides in the body, which may lead to gene mutation, cancer, deformity, etc.

Method used

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  • Microbial agent used for degrading quinclorac herbicides

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] A microbial bacterial agent for degrading quinclorac herbicides, in parts by weight, comprising: Bordetella 1.8g, Arthrobacter 1.8g, Burkholderia cepacia 1.3g, and Bacillus pallidum 1g , Pseudomonas 1.6g, Aspergillus niger 1.2g, Neurospora 1.3g, and the above-mentioned strains are all commercially available. The fermentation broths obtained after the above-mentioned strains were fermented and cultivated were mixed to form a mixed fermentation broth, 237.6 g of thalline cells were collected after centrifugation of the mixed fermentation broth, 23.76 g of skim milk and 28.5 g of sucrose were added, and then freeze-dried and mixed with 12g of cellulase, 24g of amylase, 26g of protease, 26g of lipase and 28g of bioaccelerator are compounded to obtain microbial bacterial agent.

[0015] Among them, Bordetella was inoculated in LB medium for fermentation, the culture temperature was 34°C, and cultured in a constant temperature shaking box with a rotation speed of 150r / min for...

Embodiment 2

[0023] A microbial agent for degrading quinclorac herbicides, in parts by weight, comprising: Bordetella 1.5g, Arthrobacter 2.1g, Bacillus megaterium 1g, Burkholderia cepacia 1.5 g, 1.6g of Pseudomonas, 1g of Aspergillus niger, 1.3g of Neurospora, all of which are commercially available. Mix the fermented liquids obtained after the fermentation and cultivation of the above strains respectively to form a mixed fermented liquid, collect 276.31 g of bacterial cells after centrifuging the mixed fermented liquid, add 27.6 g of skim milk and 33.16 g of sucrose, and then freeze-dry Compound with 28g of cellulase, 46g of amylase, 64g of protease, 38g of lipase and 57g of bioaccelerator to obtain microbial agent.

[0024] Among them, Bordetella was inoculated in LB medium for fermentation, the culture temperature was 34°C, and cultured in a constant temperature shaking box with a rotation speed of 130r / min for 18h;

[0025] Arthrobacter was inoculated in the culture formula as glucose...

Embodiment 3

[0032] A microbial agent for degrading quinclorac herbicides, in parts by weight, comprising: Bordetella 2g, Arthrobacter 1.8g, Bacillus megaterium 0.3g, Burkholderia cepacia 1.7 g, 1.5 g of Alcaligenes faecalis, 1.3 g of Aspergillus niger, and 1.4 g of Neurospora, all of which are commercially available. Mix the fermented liquids obtained after the fermentation and cultivation of the above-mentioned strains respectively to form a mixed fermented liquid, collect 264.21 g of bacterial cells after centrifuging the mixed fermented liquid, add 26.42 g of skim milk and 31.7 g of sucrose, and then freeze-dry Compound with 32g of cellulase, 34g of amylase, 34g of protease, 42g of lipase and 44g of bioaccelerator to obtain microbial bacterial agent.

[0033] Among them, Bordetella was inoculated in LB medium for fermentation, the culture temperature was 34°C, and cultured in a constant temperature shaking box with a rotation speed of 150r / min for 18h;

[0034] Arthrobacter was inocul...

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Abstract

The invention discloses a microbial agent used for degrading quinclorac herbicides. The microbial agent takes several strains of bordetella, arthrobacterium, bacillus megatherium, burkholderia cepacia, ochrobactrum anthropi, pseudomonas, alcaligenes faecalis, aspergillus niger and neurospora and cellulase, amylase, protease, lipase and a biological accelerating agent as raw materials, and fermented solutions obtained after the several strains of the bordetella, the arthrobacterium, the bacillus megatherium, the burkholderia cepacia, the ochrobactrum anthropi, the pseudomonas, the alcaligenes faecalis, the aspergillus niger and the neurospora undergo fermentation culture are mixed to form a mixed fermented solution, after thalli are collected by centrifugation from the mixed fermented solution, skimmed milk and sucrose are added, freeze drying is performed, and then, the thalli are compounded with the cellulase, the amylase, the protease, the lipase and the biological accelerating agentto obtain the microbial agent.

Description

technical field [0001] The invention belongs to the field of agricultural technology, in particular to a microbial bacterial agent for degrading quinclorac herbicides. Background technique [0002] Quinclorac is a highly selective hormone-type herbicide, which has the advantages of being safe to rice and having special effects on barnyard grass. It is widely used in agricultural production, but the residual time of quinclorac herbicides is relatively long and it is not easy to degrade. Easy to remain in the soil. It has been reported that quinclorac herbicides can remain in the soil for 2-3 years, even up to 4 years, and it is easy to cause phytotoxicity to subsequent crops in continuous cropping or rotation crops, especially some vegetables such as umbels. Sensitive crops such as economic crops of Solanaceae and Solanaceae will produce phytotoxicity, resulting in reduced yield, or even no harvest in severe cases. In addition, quinclorac can also be enriched in agricultura...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12N1/14C12N9/20C12N9/26C12N9/42C12N9/50B09C1/10C12R1/01C12R1/06C12R1/38C12R1/685C12R1/645
CPCB09C1/10B09C1/105C12N1/14C12N1/20C12N9/20C12N9/2411C12N9/2437C12N9/50C12Y301/01003C12Y302/01004
Inventor 王胜刘大欣曹林波
Owner SHANDONG SUNWAY LANDSCAPE TECH
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