16results about How to "Reduce abundance" patented technology

The present application relates to the field of Gastrodia elata cultivation, and particularly relates to a method for continuously planting Gastrodia elata in old planting holes. The present application effectively improves the yield of Gastrodia elata, and significantly reduces the incidence of new Gastrodia elata and the decay rate of mother Gastrodia elata by scientifically planting after rhizoma polygonati. In the present application, the breeding hole is sexual reproduction, and it does not need to cultivate armillaria bed. The armillaria, fungus material and Gastrodia elata seeds can be placed at the same time. The planting hole is asexual reproduction. Since the Gastrodia elata seed needs a large amount of nutrient elements, the armillaria bed needs to be cultivated. This operation mode can form the difference of microbial community between the breeding hole and the planting hole, which is beneficial to ecological competition, and thus improves the soil recovery and improvement.

This invention belongs to the field of pharmaceutical technology, and relates to the use of a combination of sour cherry powder and celery seed powder, and complexes containing this composition. Specifically, the sour cherry powder and celery seed powder composition is used in the preparation of drugs for treating or regulating intestinal flora imbalance caused by hyperuricemia. The preparation method of this composition is as follows: pitting sour cherries, freezing them separately with liquid nitrogen, grinding them into powder using a pulverizer, and sieving them separately to obtain sour cherry powder and celery seed powder; mixing the obtained sour cherry powder and celery seed powder at a ratio of (0.5~3):1. This invention discusses the diversity, richness, and evenness of intestinal flora through diversity analysis, community bar chart analysis, and Venn diagram analysis. The results show that this composition can increase beneficial bacteria (Prevotella, Mulliez, Lactobacillus, etc.), reduce harmful bacteria (Alternaria), improve the structure of intestinal flora, and maintain the balance of intestinal flora.

This invention provides a strain of *Lactobacillus plantarum*, and discloses the fermentation product of *Eucommia ulmoides* leaves, *Rehmannia glutinosa*, dried ginger, and *Dendrobium officinale* obtained by fermenting these herbs, along with its preparation method and applications. This fermentation product can directionally activate the phenylpropane biosynthetic pathway, inducing the generation of characteristic small molecule metabolites of polyphenols, particularly sanguranyl sulfadiazine (SSA). Moreover, compared to the raw materials, the fermentation product contains more active small molecule metabolites. Finally, experiments on zebrafish modeled with prednisone demonstrated that the fermentation product can significantly promote bone formation. The fermentation product mainly restores the expression levels of related genes to normal zebrafish gene expression levels by regulating these genes. Therefore, the fermentation product can be used to prepare foods or medicines that improve bone health.

The invention provides application of protocatechuic acid in regulating intestinal flora of procambarus clarkii and flavor amino acid composition in muscle of the procambarus clarkii, and belongs to the technical field of development of aquatic feed additives. It is found that the protocatechuic acid can reduce the abundance of Vibrionaceae in the intestinal tract level of the procambarus clarkii, improve the abundance of Rhodobacteroceae, Lactobacillus and Bacillus, reduce the abundance of Vibrio in the intestinal tract level of the procambarus clarkii, and improve the abundance of Halocynthiibacter and Ruegeria, so that the composition of flavor amino acids in the muscles of the procambarus clarkii is improved. The invention further provides a procambarus clarkii feed and application thereof in procambarus clarkii culture, the content of flavor amino acids in procambarus clarkii can be effectively increased, the muscle quality of procambarus clarkii is improved, the cost is low, and the culture method is simple.

The invention relates to the technical field of compositions, and particularly discloses a composition for improving EoE and application thereof. The composition for improving EoE comprises choline, taurine and a selenium source. In the composition for improving EoE provided by the invention, each raw material component is reasonably designed, and the effect of remarkably improving EoE is achieved by fully exerting a synergistic effect. Research shows that the composition can significantly improve EoE, which is mainly reflected in that the composition can reduce infiltration and activation of eosinophilic granulocytes in esophageal tissues, effectively reverse esophageal epithelial hyperplasia, reduce levels of cell factors such as IL-13 and the like, improve abundance of bifidobacteria in intestinal tracts and reduce abundance of enterobacter.

The application discloses a DNA sulfur-modified bacillus subtilis and application thereof in alleviating alcoholic diseases, and relates to the field of biochemistry.The application provides a DNA sulfur-modified bacillus subtilis, which is named as Bacillus subtilis E10-1 and has a preservation number of CCTCC NO: M20253036.It is verified through animal experiments that the DNA sulfur-modified bacillus subtilis E10-1 is more resistant to alcohol than a strain in which sulfur modification is knocked out, can reduce the levels of indexes such as glutathione pyrotransaminase, glutathione transaminase and urea nitrogen in serum of a host after drinking alcohol, and the level of interferon gamma, can increase the levels of total protein, superoxide dismutase and glutathione peroxidase, can alleviate oxidative stress damage caused by alcohol, can reduce inflammatory reactions of various organ tissues, can maintain normal physiological functions of cells, can repair intestinal barriers, and can maintain intestinal flora homeostasis, so that alcoholic related diseases can be prevented and / or treated.

The application discloses a marker for screening aggressive social harmful behavior, a screening method and device and a storage medium, and relates to the technical field of aggressive social behavior screening. The reagent comprises a reagent for detecting relative abundance data of a microbial combination at the genus level. When the 13 kinds of bacteria are used for screening aggressive social harmful behavior, the screening accuracy is extremely high, and the screening method is non-invasive and objective. When the 13 kinds of bacteria are combined with social demographic information as screening factors of aggressive harmful social behavior, the screening accuracy is also extremely high. The application provides a new way for screening and intervention of aggressive social harmful behavior.

The application discloses a method for removing antibiotic resistance genes in straw sludge compost and application thereof, and belongs to the technical field of composting. In order to remove antibiotic resistance genes in municipal sludge, the application provides a method for removing antibiotic resistance genes in straw substrate sludge compost, specifically, antibiotic resistance genes are removed through combined action of strong oxidation and high temperature in aerobic composting. The method significantly reduces the abundance of antibiotic resistance genes in the compost sample on the basis of traditional aerobic composting technology, and reduces the potential risk of human health caused by recycling of the compost product in combination with an ozone oxidation technology and corn straw as a carbon source.

The application discloses application of Bacteroides oleamicus in preparation of drugs for preventing or treating colorectal cancer. The abundance of Bacteroides oleamicus in the present application is significantly reduced in tissues and feces of colorectal cancer patients. In a mouse model, Bacteroides oleamicus can be used for intragastric administration to inhibit the growth of colorectal cancer, and has no significant influence on the weight of mice, the secretion of inflammatory factors in serum and the expression of barrier proteins. The present application proves that Bacteroides oleamicus has important significance for early diagnosis, prevention and treatment of colorectal cancer, and provides a clearer path for clinical application and transformation.

The invention discloses application of fiveleaf gynostemma herb polysaccharide in preparation of medicine for treating ulcerative colitis, the main structure of the fiveleaf gynostemma herb polysaccharide is shown in the specification, research proves that the prepared fiveleaf gynostemma herb polysaccharide has an excellent treatment effect on ulcerative colitis for the first time, activation of NLRP3 inflammasome is inhibited by activating an NRF2 anti-oxidation pathway, and the curative effect of the fiveleaf gynostemma herb polysaccharide on ulcerative colitis is improved. The bacterial flora abundance of bacteroides and thick-walled mycophylum is increased, and the bacterial flora abundance of proteobacteria is reduced, so that the ROS level is reduced, the inflammatory response is reduced, and the intestinal mucosal barrier repair of DSS mice is promoted.

The application discloses a double-layer microcapsule containing lactobacillus plantarum and a preparation method and application thereof, and application of lactobacillus plantarum EASY 0352 in preparation of anxiolytic and depressive products. 2+ The metal-phenol network coating is formed on the surface of the bacteria by coordination self-assembly, the single-cell coating microcapsule can significantly enhance the adhesion of lactobacillus plantarum EASY 0352 to intestinal epithelial cells, and is beneficial to the colonization of lactobacillus plantarum EASY 0352 in the intestinal tract; on the basis of the single-cell coating microcapsule, the application further coats transglutaminase (TG) and whey protein concentrate (WPC) composite wall materials to form a double-layer microcapsule; and simulation of gastric and intestinal fluid digestion experiment results show that the double-layer microcapsule prepared by the application can significantly improve the survival rate of lactobacillus plantarum EASY 0352 in simulated gastric and intestinal fluid, and effectively protects the probiotics to pass through the gastrointestinal tract smoothly.

The application discloses a neutrophil-mediated engineered cross-nanovesicle delivery platform and application thereof, and belongs to the technical field of biological medicines. The delivery platform is composed of an exogenous cholesterol-rich LNV-SyBV hybrid vesicle and polimyxin B loaded by the exogenous cholesterol-rich LNV-SyBV hybrid vesicle. The exogenous cholesterol-rich LNV-SyBV hybrid vesicle is prepared from a plant-derived anti-inflammatory vesicle, an attenuated bacterial vesicle and exogenous cholesterol. The plant-derived anti-inflammatory vesicle is a nanovesicle LNV from lemon. The attenuated bacterial vesicle is a synthetic vesicle SyBV from Escherichia coli. The neutrophil-mediated engineered cross-nanovesicle delivery platform and application thereof provide an innovative strategy for the clinical treatment of carbapenem-resistant gram-negative bacterial infections through targeted delivery, antibacterial and anti-inflammatory dual functions and low immunotoxicity.

The invention discloses a fermented feed for promoting ruminants to efficiently utilize soluble proteins as well as a preparation method and application of the fermented feed, and belongs to the technical field of animal feeds. The fermented feed is prepared by fermenting a substrate consisting of cottonseed meal, corn flour and bran with a compound bacteria solution, and the substrate comprises the following components in percentage by mass: 5-31% of cottonseed meal, 2-31% of corn flour and 38-93% of bran; the mass of the composite bacterial liquid is 8-10% of that of the substrate, and the composite bacterial liquid is prepared by compounding a candida tropicalis bacterial liquid and an enterococcus faecalis bacterial liquid according to a volume ratio of 1: 1, wherein the concentrations of the candida tropicalis bacterial liquid and the enterococcus faecalis bacterial liquid are both 108 CFU / mL. The preparation method comprises the following steps: crushing and sieving the substrate, mixing, adjusting the water content to 40%, spraying the compound bacteria liquid, uniformly mixing, and fermenting at a constant temperature of 30 DEG C. According to the invention, the substrate ratio and the fermentation process are optimized, the utilization efficiency of soluble protein by ruminants is improved, the rumen fermentation environment is improved, meanwhile, the nitrogen emission is reduced, and economic benefits and environmental protection values are achieved in animal production.

The invention belongs to the technical field of veterinary biological products, and discloses chlorella pyrenoidosa CV-5, a probiotic microcapsule and application of the probiotic microcapsule in preparation of products for improving intestinal health of calves. The preservation number of the chlorella pyrenoidosa CV-5 provided by the invention is CGMCC (China General Microbiological Culture Collection Center) No.15206, and the Latin name of the chlorella pyrenoidosa CV-5 provided by the invention is Chlorella pyrenoidosa. Compared with other algae, the chlorella pyrenoidosa CV-5 provided by the invention has remarkable advantages in the aspect of improving the intestinal health of calves. On the basis, chlorella pyrenoidosa CV-5 powder and pectin are matched with glucose oxidase to serve as wall materials, dominant probiotic strains, sodium butyrate, trypsin and specific prebiotics serve as core materials, and the feed capable of remarkably improving the survival rate of the probiotics in the calf wrinkled stomach environment, improving the survival rate of the probiotics in the calf wrinkled stomach environment and improving the survival rate of the probiotics in the calf wrinkled stomach environment is further developed. Meanwhile, the abundance of pathogenic bacteria in intestinal tracts of the diarrhea calves is reduced. The feed additive is suitable for the livestock breeding industry, and especially has a good application prospect in the field of improving the intestinal health of the calves.

The invention belongs to the technical field of biological medicines, and relates to application of a histone deacetylase inhibitor HDACi in preparation of a medicine for preventing and / or treating hyperammonemia-induced encephalopathy. According to the invention, a histone deacetylase inhibitor HDACi is used as a new medicine, the HDACi can enter a central nervous system, and the GS is triggered to be degraded through a proteasome mediated by CRBN E3 ubiquitin ligase by improving the acetylation level of a key enzyme, namely glutamine synthetase (GS), of astrocytes, so that the GS protein abundance is reversibly reduced, and the GS protein abundance is reduced. The application has the advantages that pathological accumulation of toxic metabolite glutamine can be reduced from the source, cerebral edema, autophagy-lysosomal dysfunction, neuroinflammation and neuron injury caused by the pathological accumulation can be synchronously improved, severe neurotoxicity such as persistent enzyme inhibition and acute epilepsy caused by irreversible inhibitors such as MSO can be avoided, clinical transformation risks can be obviously reduced, and the safety is high.

The invention provides a method for reducing abundance of antibiotic resistance genes in soil and application of the method, and belongs to the technical field of environmental pollution treatment and soil remediation. Lignin is clearly used as a main active component for the first time, the abundance of multiple ARGs in soil can be remarkably reduced, and the effect is stable. The method provided by the invention has the advantage of simplicity and convenience in operation, only the lignin and the soil need to be simply mixed and proper humidity is maintained, complicated equipment or harsh conditions are not needed, and the method is easy to implement in fields such as farmlands and farms.