77results about How to "Reduce abundance" patented technology

The invention relates to application of breast milk oligosaccharides to improvement of intestinal flora of mothers and infants, in particular to application of 2 '-fucosyllactose to preparation of food which is taken by a mother and contributes to regulation of intestinal flora of the mother and offspring thereof, and the mother is in a gestation period and / or a lactation period. The invention is provided through a large amount of research; when a mother supplements a certain amount of 2 '-fucosyllactose in a gestation period and / or a lactation period, the abundance of beneficial bacteria in intestinal tracts of the mother can be remarkably improved, the content of harmful bacteria is reduced, meanwhile, the abundance of beneficial bacteria in intestinal tracts of filial generations of the mother is remarkably improved, and the content of harmful bacteria is reduced; particularly, the abundance of some beneficial bacteria which cannot be directly eaten in the intestinal tracts of the mother and the offspring can be adjusted.

The application of the animal bifidobacterium subsp. Lactis BX-245 in relieving the inflammatory bowel disease is characterized in that the animal bifidobacterium subsp. Lactis BX-245 is preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation number is CGMCC No.26973. The invention also discloses an application of the animal bifidobacterium subsp. Lactis BX-245 in relieving the inflammatory bowel disease. The research aims at discussing the relieving effect of the animal bifidobacterium subsp. Lactis BX-245 on IBD mice induced by dextran sodium sulfate (DSS), and 6-week male C57BL / 6J mice which normally eat and drink water are randomly divided into a normal group (NC group), a model group (IBD group), a common yoghourt (FM group), an animal bifidobacterium subsp. Lactis BX-245 bacterial powder group (BX-245 group) and BX-245 yoghourt (BFM group). The relieving effect of the animal bifidobacterium subsp. Lactis BX-245 and the fermented milk of the animal bifidobacterium subsp. Lactis BX-245 on the symptom of the mouse inflammatory bowel disease is evaluated through weight change, immune factors, targeted metabonomics and metagenomics, and data support is provided for application of the animal bifidobacterium subsp. Lactis BX-245 in products.

The invention discloses lactobacillus reuteri CCFM1288 for relieving depressive mood and application of the lactobacillus reuteri CCFM1288, and belongs to the technical field of microorganisms. The lactobacillus reuteri CCFM1288 has the tryptophan metabolism capability, and can survive for 7 days in the intestinal tract; after the lactobacillus reuteri CCFM1288 intervenes a depressive mouse, anxiety-like and depression-like behaviors of the mouse can be obviously relieved, flora alpha-diversity is improved, intestinal symbiotic bacteria are promoted to secrete indole substances, intestinal homeostasis is maintained, hypothalamic-pituitary-adrenal axis hyperfunction is relieved, dopamine and serotonin neurotransmitter nervous system functions are improved, and the effect of preventing and treating depression is achieved. The neurotransmitter release is promoted, and the anti-depression effect is achieved. In clinical tests, intervention of Lactobacillus reuteri CCFM1288 can significantly improve the depressive symptom of MDD patients, and a new safe alleviation approach is provided for depression treatment.

The invention belongs to the technical field of microorganisms, and particularly relates to clostridium butyricum and application thereof, and the preservation number of the clostridium butyricum is CGMCC (China General Microbiological Culture Collection Center) No.27853. According to the present invention, with the application of the bacterial strain, the species diversity of the host intestinal flora can be significantly reduced, the Firmicite / Bacteroidetes ratio can be significantly increased, the abundance of harmful bacteria such as Escherichia coli and Salmonella bacteria can be reduced, and the abundance of beneficial bacteria can be improved; in addition, the yield of butyric acid of the clostridium butyricum is larger than or equal to 1.5 g / L, and the activity of amylase in fermentation liquor of the clostridium butyricum is 16.04 + / -0.46 U / mL. When the feed additive is used in feed, intestinal functions of organisms can be effectively improved.

The invention discloses a medicine for treating renal cell carcinoma and application thereof, and belongs to the field of biomedicine, the medicine comprises a reagent capable of ubiquitination degradation of WEE1, FBXO2 overexpression can prevent proliferation and movement of renal cell carcinoma cells, and FBXO2 can reduce abundance of WEE1 through ubiquitination degradation; the interaction and ubiquitination degradation of the WEE1 are mediated by the FBA structural domain of the FBXO2; fBXO2 degrades WEE1 through ubiquitination to inhibit RCC canceration.

The invention belongs to the technical field of drug delivery and food medicine, and particularly relates to resistant starch of intestinal targeted slow-release polyphenol and a preparation method and application of the resistant starch of the intestinal targeted slow-release polyphenol. Performing annealing recrystallization on the heat-dispersed high-amylose corn starch in an ethanol solution to obtain single-spiral structure resistant corn starch; and self-assembling the resistant corn starch with the single-spiral structure and the quinoa polyphenol extract in an alcohol-water system to obtain the intestinal targeted slow-release polyphenol resistant starch. According to the resistant starch of the intestinal targeted slow-release polyphenol prepared by the method disclosed by the invention, the specific starch spiral structure can realize the intestinal targeted controlled release of the polyphenol contained in the intestinal targeted slow-release polyphenol, so that the loss of phenolic compounds in the gastrointestinal digestion process is reduced, and the accurate and continuous release of the polyphenol compounds in the intestinal digestion stage is realized; the residence time of the polyphenol in the intestinal tract is prolonged, so that the bioavailability of the polyphenol is improved.

The invention discloses bifidobacterium breve with hypoglycemic capacity and application, a product and a method thereof, and belongs to the technical field of microorganisms. The invention provides a bifidobacterium breve (Bifidobacterium breve) strain BBr37 with the blood sugar reducing capacity, and the preservation number of the bifidobacterium breve strain BBr37 is CGMCC (China General Microbiological Culture Collection Center) NO.32423. The invention also provides an application of the strain BBr37 in preparation of hypoglycemic drugs or health-care foods with an auxiliary hypoglycemic function or bacteriostatic agents or non-treatment-destination bacteriostasis, and a product and a method based on the strain BBr37. The strain BBr37 disclosed by the invention has a relatively strong inhibition effect on escherichia coli, staphylococcus aureus, salmonella, haemophilus parasuis, haemophilus parainfluenza, campylobacter jejuni and enterocolitis yarrowia, and can effectively achieve the effects of reducing blood sugar and regulating intestinal flora.

The invention relates to the technical field of biology, in particular to a method for solid state fermentation of bagasse by using white rot fungi, which comprises the following steps: (1) activation and propagation of strains: pouring the white rot fungi into a PDA culture medium for activation and propagation; (2) raw material preparation: air-drying fresh bagasse, adding water to adjust the water content of the bagasse to 60-70%, and adjusting the pH to 4-6 with glacial acetic acid; and (3) preparing the bagasse feed, namely mixing the white rot fungi subjected to expanding culture according to 1-5% of the weight of an air-dried substance with bagasse of which the initial pH is adjusted to be 4-6, adding 1-5% of soluble sugar, and fermenting for 10-45 days to obtain the fermented bagasse feed. According to the white rot fungus fermented bagasse prepared by the method disclosed by the invention, the hemicellulose content of the bagasse can be reduced to 26.62%-22.45%, the lignin content can be reduced to 10.67%-8.66%, the crude protein content of the bagasse can be increased to 1.61%-2.12%, the lactic acid content can be increased to 26.57 mg / L-181.69 mg / L, and high-quality feed application of the bagasse can be realized.

The present application relates to the field of Gastrodia elata cultivation, and particularly relates to a method for continuously planting Gastrodia elata in old planting holes. The present application effectively improves the yield of Gastrodia elata, and significantly reduces the incidence of new Gastrodia elata and the decay rate of mother Gastrodia elata by scientifically planting after rhizoma polygonati. In the present application, the breeding hole is sexual reproduction, and it does not need to cultivate armillaria bed. The armillaria, fungus material and Gastrodia elata seeds can be placed at the same time. The planting hole is asexual reproduction. Since the Gastrodia elata seed needs a large amount of nutrient elements, the armillaria bed needs to be cultivated. This operation mode can form the difference of microbial community between the breeding hole and the planting hole, which is beneficial to ecological competition, and thus improves the soil recovery and improvement.

The invention provides a feed traditional Chinese medicine additive for improving chicken quality of broiler chickens and application thereof, and relates to the field of animal feeds, and the feed traditional Chinese medicine additive provided by the invention comprises the following raw material components in parts by mass: 10-30 parts of radix tetrastigme stems and leaves, 1-2 parts of pomegranate rind, 0.5-1 part of dried orange peel and 0.5-1 part of liquorice. By using the feed traditional Chinese medicine additive and the broiler feed provided by the invention, the contents of flavor amino acids such as glutamic acid, alanine and threonine and essential amino acids such as phenylalanine, lysine and isoleucine can be increased, the amino acid composition of broilers is improved, the flavor of broiler chicken is improved, and the quality of broiler chicken is improved; the abundance of beneficial bacteria such as Prevoteraceae UCG001 and the like can be improved, the abundance of harmful bacteria such as Mucispirillum and the like can be reduced, and meanwhile, the intestinal nutrition transfer can be enhanced and the inflammatory response in vivo can be relieved.

The invention discloses a morchella esculenta-ganoderma lucidum cultivation method for improving continuous cropping soil of morchella esculenta, and relates to the technical field of morchella esculenta cultivation, and the method comprises the following steps: in the same land parcel, planting a first round of medium-mushroom morchella esculenta No.1 in November of the first year, and planting ganoderma lucidum after planting a second round of medium-mushroom morchella esculenta No.1 in November of the second year; after harvesting of the medium mushroom morchella No.1 every time, the soil is turned over and aired. By means of the method, the morchella planting frequency can be increased, the morchella yield can be increased, fungus communities in soil can be adjusted by planting ganoderma lucidum twice, vacant land parcels are not needed, the land utilization rate is increased, flora distribution can be adjusted, and ganoderma lucidum harvest can be obtained.

The invention belongs to the field of modern agricultural technology and relates to a method for soil improvement, and specifically relates to a method for improving saline-alkali land, wherein a composite bacterial agent of Huajinpu alkaline halobacter BH‑8 and coal gangue is applied to saline-alkali land soil, wherein the concentration of Huajinpu alkaline halobacter BH‑8 in the composite bacterial agent is 1×10 9 ~3×10 9 CFU / mL, the concentration of coal gangue particles is 5-10g / L; the present invention uses Huajinpu alkaline halobacter and coal gangue to prepare a composite bacterial agent for saline-alkali land improvement for the first time. By comparing with the blank control group, coal gangue alone, and microorganisms alone, the combination of Huajinpu alkaline halobacter and coal gangue improves the soil fertility and various enzyme activities of saline-alkali land, and optimizes the structure and function of soil microbial communities in saline-alkali land. The method of the present invention is scientific and reliable, has good repeatability, has important scientific value, provides an important reference for saline-alkali land improvement, helps to play an important role in saline-alkali land improvement, and improves the efficiency of saline-alkali land improvement.

The invention discloses Lactobacillus crispatus and its use. The Lactobacillus crispatus is Lactobacillus crispatus chen-01 (Lactobacillus crispatus chen-01), and its deposit number is CGMCC NO.23396. Lactobacillus crispatus can be used to prepare medicines, and the medicines are used to treat and / or prevent HPV infection and vaginal infection. After the medicine prepared by the Lactobacillus crispatus is locally transplanted into the vagina of the present invention, the HPV viral load can be effectively reduced, the HPV clearance rate can be significantly improved, vaginitis can be effectively improved, and the diversity and abundance of vaginal flora can be significantly reduced; in addition, local vaginal transplantation of Lactobacillus crispatus can also significantly improve the vaginal microecological composition, which is close to that of normal healthy people.

This invention belongs to the field of pharmaceutical technology, and relates to the use of a combination of sour cherry powder and celery seed powder, and complexes containing this composition. Specifically, the sour cherry powder and celery seed powder composition is used in the preparation of drugs for treating or regulating intestinal flora imbalance caused by hyperuricemia. The preparation method of this composition is as follows: pitting sour cherries, freezing them separately with liquid nitrogen, grinding them into powder using a pulverizer, and sieving them separately to obtain sour cherry powder and celery seed powder; mixing the obtained sour cherry powder and celery seed powder at a ratio of (0.5~3):1. This invention discusses the diversity, richness, and evenness of intestinal flora through diversity analysis, community bar chart analysis, and Venn diagram analysis. The results show that this composition can increase beneficial bacteria (Prevotella, Mulliez, Lactobacillus, etc.), reduce harmful bacteria (Alternaria), improve the structure of intestinal flora, and maintain the balance of intestinal flora.

This invention provides a strain of *Lactobacillus plantarum*, and discloses the fermentation product of *Eucommia ulmoides* leaves, *Rehmannia glutinosa*, dried ginger, and *Dendrobium officinale* obtained by fermenting these herbs, along with its preparation method and applications. This fermentation product can directionally activate the phenylpropane biosynthetic pathway, inducing the generation of characteristic small molecule metabolites of polyphenols, particularly sanguranyl sulfadiazine (SSA). Moreover, compared to the raw materials, the fermentation product contains more active small molecule metabolites. Finally, experiments on zebrafish modeled with prednisone demonstrated that the fermentation product can significantly promote bone formation. The fermentation product mainly restores the expression levels of related genes to normal zebrafish gene expression levels by regulating these genes. Therefore, the fermentation product can be used to prepare foods or medicines that improve bone health.

The invention discloses PHGG with specific molecular weight as well as a preparation method and application thereof, and relates to the technical field of PHGG preparation. The method comprises the following steps: (1) mixing guar gum, cellulase and mannase in a phosphate buffer solution, and then stirring for enzymolysis to obtain a guar gum enzymolysis solution; (2) performing enzyme deactivation and sterilization on the obtained guar gum enzymolysis solution, and performing freeze drying after filtration to obtain PHGG; in the step (2), the molecular weight of the PHGG is 5 to 500 kDa. Compared with the prior art, the operation is simpler, and the molecular weight of the prepared PHGG is more accurate.

The invention provides application of protocatechuic acid in regulating intestinal flora of procambarus clarkii and flavor amino acid composition in muscle of the procambarus clarkii, and belongs to the technical field of development of aquatic feed additives. It is found that the protocatechuic acid can reduce the abundance of Vibrionaceae in the intestinal tract level of the procambarus clarkii, improve the abundance of Rhodobacteroceae, Lactobacillus and Bacillus, reduce the abundance of Vibrio in the intestinal tract level of the procambarus clarkii, and improve the abundance of Halocynthiibacter and Ruegeria, so that the composition of flavor amino acids in the muscles of the procambarus clarkii is improved. The invention further provides a procambarus clarkii feed and application thereof in procambarus clarkii culture, the content of flavor amino acids in procambarus clarkii can be effectively increased, the muscle quality of procambarus clarkii is improved, the cost is low, and the culture method is simple.

The invention relates to the technical field of breeding of adult eriocheir sinensis, in particular to application of a new wheat-crab ecological planting and breeding mode to improvement of microbial communities in an adult crab breeding environment, which comprises the following steps: planting wheat in a field, digging ditches around to protect eriocheir sinensis, and performing field operation in the growth period of wheat; after wheat is harvested, straw is removed, then land is ploughed, and water is injected for grass planting; after 15 days, Chinese mitten crabs are bred in the wheat stubble field; through the new wheat-crab ecological planting and breeding mode, the microorganism composition of the culture environment of the eriocheir sinensis is improved, the change rule of microorganisms of water quality, water body and bottom mud under the new wheat-crab ecological planting and breeding mode is mastered, and the culture mode of adult crabs is enriched.

The invention discloses an energy brick for energy supplementation in the lactation period of sows, and belongs to the technical field of energy supplements. The energy brick comprises the following components: grease, carbohydrate, a protein raw material, amino acid, an element supplement, vitamins and an additive. The energy brick for supplementing energy in the lactation period of the sows does not contain antibiotics and chemical synthetic drugs, does not cause adverse effects on growth of the sows, and does not indirectly affect human health; the energy brick for supplementing energy in the lactation period of the sows is formed in a brick form, so that the energy brick is convenient to store and transport, the sows mainly eat food in a licking mode, the feeding speed of the sows can be slowed down, the feeding frequency is reduced, and meanwhile, continuous supply of energy can be effectively guaranteed.

The present invention relates to the use of human milk oligosaccharides to improve the intestinal flora of mothers and infants, and specifically to the use of 2'-fucosyllactose in the preparation of a food that helps regulate the intestinal flora of the mother and her offspring through intake by the mother, wherein the mother is in pregnancy and / or lactation. After a large number of studies, the present invention proposes that the mother can significantly increase the abundance of beneficial bacteria in the intestine and reduce the content of harmful bacteria by supplementing a certain amount of 2'-fucosyllactose during pregnancy and / or lactation, and at the same time significantly increase the abundance of beneficial bacteria in the intestine of the offspring and reduce the content of harmful bacteria, especially the abundance of some non-directly edible beneficial bacteria in the intestine of the mother and the offspring can be regulated.

The invention relates to the technical field of compositions, and particularly discloses a composition for improving EoE and application thereof. The composition for improving EoE comprises choline, taurine and a selenium source. In the composition for improving EoE provided by the invention, each raw material component is reasonably designed, and the effect of remarkably improving EoE is achieved by fully exerting a synergistic effect. Research shows that the composition can significantly improve EoE, which is mainly reflected in that the composition can reduce infiltration and activation of eosinophilic granulocytes in esophageal tissues, effectively reverse esophageal epithelial hyperplasia, reduce levels of cell factors such as IL-13 and the like, improve abundance of bifidobacteria in intestinal tracts and reduce abundance of enterobacter.

The invention relates to an application of breast milk oligosaccharide in preparation of a preparation for inhibiting proliferation of intestinal tract Ruminococcus, the breast milk oligosaccharide is 2 '-FL and / or 6'-SL, the relative abundance of the intestinal tract Ruminococcus can be obviously reduced, the proliferation of the intestinal tract Ruminococcus can be inhibited, and the breast milk oligosaccharide can be applied to preparation of the preparation for inhibiting the proliferation of the intestinal tract Ruminococcus. The breast milk oligosaccharide is 2 '-FL or a composition of 2'-FL and 6 '-SL, compared with 2'-FL or a composition of 2 '-FL and 6'-SL, the breast milk oligosaccharide has a better inhibition effect on intestinal tract Ruminococcus, can be applied to preparation of a preparation for inhibiting abnormal proliferation of intestinal tract Ruminococcus of a patient suffering from the inflammatory bowel disease, and is particularly applied to preparation of a preparation for preventing enteritis and maintaining intestinal health.

The invention discloses an application of cordyceps guangdongensis sporocarp polysaccharide in preparation of a product for treating and improving diabetic nephropathy. Experimental results show that the cordyceps guangdongensis sporocarp polysaccharide can treat and improve the diabetic nephropathy, improve the renal function, reduce the levels of serum urea nitrogen and serum creatinine, and reduce the level of uremia toxin indolesulfuric acid phenol in the plasma of diabetic mice; kidney inflammation is improved, and mRNA expression of IL-6, TNF-alpha, IL-1beta and MCP-1 in the kidney is reduced; the oxidative stress and fibrosis of the kidney are inhibited, and the mRNA expression of Rac1, keap1, Ncf2 and Nsf11c of the kidney is reduced; the abundance of pathogenic bacteria in intestinal tracts and kidneys is reduced, and the pathogenic bacteria are mainly Escherichia.

The invention provides hydrothermal carbon-based biological nano-selenium as well as a preparation method and application thereof, the hydrothermal carbon-based biological nano-selenium is prepared by taking a selenium-resistant functional strain as a chassis cell and inorganic selenium as a precursor substance; then, with garden waste as a carbon-based raw material and biological nano-selenium as a selenium source, synthesizing hydrothermal carbon-based biological nano-selenium through a one-step hydrothermal carbonization method; finally, the obtained hydrothermal carbon-based biological nano-selenium is applied to harmful substance removal and biomass energy production of an arsenic-containing breeding wastewater anaerobic digestion system. The preparation process of the hydrothermal carbon-based biological nano-selenium is the combination of biological fermentation and hydrothermal reaction, the method is green, safe and low in energy consumption, the arsenic content in an anaerobic digestion final product of the aquaculture wastewater and the abundance of antibiotic resistance genes (ARGs) and pathogenic bacteria can be effectively reduced by adding the hydrothermal carbon-based biological nano-selenium prepared by the method into the arsenic-containing aquaculture wastewater, and the yield of the aquaculture wastewater is improved. Meanwhile, the methane yield is increased.

The invention relates to the technical field of biological detection, in particular to a method for identifying young pigeons and laying pigeons based on metagenome sequencing. According to the method, on the basis of metagenome sequencing of the DNA sample of the pigeon faeces, the intestinal microbial flora characteristics and the antibiotic resistance gene characteristics are used as identification indexes, the young pigeons and the laying pigeons can be effectively distinguished and identified, meanwhile, a macro whole genome marker is provided for analysis of the intestinal microorganisms of the pigeons, and assistance is provided for research on healthy feeding and breeding of meat pigeons. By utilizing the method disclosed by the invention, the microbial assembly with higher resolution can be generated, and the microbial classification group with lower abundance and the function thereof can be identified, so that more microbial diversity in the samples and among the samples can be revealed.

The invention relates to the technical field of harmless treatment of livestock and poultry manure, in particular to a method for reinforcing and reducing plasmid-mediated resistance genes by using mixed magnetic resin. The method comprises the following steps: adding livestock and poultry manure and mixed magnetic resin into a fermentation tank, controlling the pH value of a system in the fermentation tank to be 7-8, and controlling the temperature of a reaction tank to be 37 + / -1 DEG C for anaerobic digestion; wherein the addition amount of the mixed magnetic resin is 0.1-0.5 g / kg of total suspended solids, and the mixed magnetic resin is a mixture of magnetic cation exchange resin and magnetic anion exchange resin in a mass ratio of 2: 3-3: 2. The method provided by the invention is used for treating the livestock and poultry manure, and provides a new technical support for reducing resistance genes and controlling transmission of the resistance genes.

The invention discloses related application of fructus lycii fermented wine in regulating intestinal flora, and relates to the field of fructus lycii wine. It is found for the first time that the fructus lycii fermented wine can improve the relative abundance of ackermann bacteria, lactobacillus, bifidobacterium, flavonolysis bacteria and blue-green algae bacteria and meanwhile reduce the relative abundance of escherichia, shigella, proteusbacillus vulgaris, pseudomonas and klebsiella; the content of short-chain fatty acids in intestinal tracts can be remarkably increased, and the probiotic can be applied to the field of foods as prebiotics.

The invention discloses the application of abalone visceral glycoprotein nano-selenium in the preparation of an aquatic feed additive for regulating the intestinal flora of tilapia. The abalone visceral glycoprotein nano-selenium of the invention can be used as an aquatic feed additive to improve the intestinal microbial diversity of tilapia, significantly increase the abundance of some beneficial bacteria such as Ceti in the intestinal tract of tilapia, significantly reduce the abundance of conditional pathogens such as Plesiomonas and Citrobacter and the abundance of potential pathogen Citrobacter freundii, and significantly increase the level of pyrrole-2-carboxylic acid in the intestinal tract of tilapia.

The application discloses a DNA sulfur-modified bacillus subtilis and application thereof in alleviating alcoholic diseases, and relates to the field of biochemistry.The application provides a DNA sulfur-modified bacillus subtilis, which is named as Bacillus subtilis E10-1 and has a preservation number of CCTCC NO: M20253036.It is verified through animal experiments that the DNA sulfur-modified bacillus subtilis E10-1 is more resistant to alcohol than a strain in which sulfur modification is knocked out, can reduce the levels of indexes such as glutathione pyrotransaminase, glutathione transaminase and urea nitrogen in serum of a host after drinking alcohol, and the level of interferon gamma, can increase the levels of total protein, superoxide dismutase and glutathione peroxidase, can alleviate oxidative stress damage caused by alcohol, can reduce inflammatory reactions of various organ tissues, can maintain normal physiological functions of cells, can repair intestinal barriers, and can maintain intestinal flora homeostasis, so that alcoholic related diseases can be prevented and / or treated.

The application discloses a marker for screening aggressive social harmful behavior, a screening method and device and a storage medium, and relates to the technical field of aggressive social behavior screening. The reagent comprises a reagent for detecting relative abundance data of a microbial combination at the genus level. When the 13 kinds of bacteria are used for screening aggressive social harmful behavior, the screening accuracy is extremely high, and the screening method is non-invasive and objective. When the 13 kinds of bacteria are combined with social demographic information as screening factors of aggressive harmful social behavior, the screening accuracy is also extremely high. The application provides a new way for screening and intervention of aggressive social harmful behavior.