202results about How to "Reduce abundance" patented technology

The invention relates to a manufacture technology of a lithium ion battery cathode material and specifically provides a high density lithium ion battery cathode material lithium cobalt oxide and a preparation method thereof. The high density lithium ion battery cathode material lithium cobalt oxide of the invention has a chemical formula of Li1+xCo(1-a-b-c) MgaTibAlcFdO(2-d), wherein a, b, c, d and x satisfy relations of: 0<=a<=0.03, 0.001<=b<=0.02, 0<=c<=0.01, 0<=d<=0.02 and 0<=x<=0.08; and a preferable value of a, a preferable value of b, a preferable value of c and a preferable value of d satisfy relations of: 0.005<=a<=0.01, 0.002<=b<=0.01, 0<=c<=0.005 and 0<=d<=0.005; and a cation M is doped or cladded by one or a composition of several selected from magnesium, titanium and aluminium. The invention can enhance stability of material structure, cycle performance and safety performance of the lithium ion battery as well as effectively increase space utilization rate of the material and compacted density of the cathode material, thereby substantially raising volume energy density of the material.

The invention provides a non-woven base composite membrane for lithium isotope separation and a preparation method thereof, as well as a lithium isotope separation method by using a membrane chromatography. The non-woven base composite membrane takes a non-woven fabric as a porous support body or a micropore membrane, a coating is prepared on the non-woven fabric by taking a crown ether graft polymer or a Kryptofix graft polymer with a lithium isotope separation effect as a film forming matter, or a coating can be prepared on the non-woven fabric by blending crown ether or Kryptofix and derivates of the crown ether or the Kryptofix into a film-formation polymer solution, and the coating and a base membrane are combined to form a composite separated membrane; the composite separated membrane is taken as a membrane chromatography medium stationary phase, so as to separate lithium isotopes by using the membrane chromatography. The non-woven base composite membrane or the lithium isotope separation method by using the membrane chromatography can effectively improve the contact and coupling efficiency of crown ether molecules and Lithium Ions in the separation process for solid-liquid extraction lithium isotopes, and realize the greenization, serialization and high efficient of the process of lithium isotope separation.

Provided is a high-purity copper or high-purity copper alloy sputtering target of which the purity is 6N or higher and in which the content of the respective components of P, S, O and C is 1 ppm or less, wherein the number of nonmetal inclusions having a particle size of 0.5 μm or more and 20 μm or less is 30,000 inclusions / g or less. As a result of using high-purity copper or high-purity copper alloy from which harmful inclusions of P, S, C and O system have been reduced as the raw material and controlling the existence form of nonmetal inclusions, the present invention addresses a reduction in the percent defect of wirings of semiconductor device formed by sputtering a high-purity copper target so as to ensure favorable repeatability.

The invention relates to bifidobacterium longum subsp. infantis CCFM687 as well as fermented food and application thereof. The bifidobacterium longum subsp. infantis CCFM687 provided by the inventioncan improve the depressive behavior of a depression mouse and raise the level of 5-hydroxytryptamine, 5-hydroxytryptophane and brain-derived neurotrophic factor in the brain of the depression mouse; moreover, the content of butyric acid in the intestines of the depression mouse is increased; the abundance of intestinal desulfovibrio is lowered while the abundance of bifidobacterium and S24-7 family is raised, the intestinal flora alpha-diversity is improved, the intestinal flora disturbance of the depression mouse is relieved, and the occurrence of autism, inflammatory bowel disease, obesity,diabetes mellitus type I and the like is reduced; the level of mRNA simulating tryptophan hydroxylase in enterochromaffin cell is raised, the secretion of 5-hydroxytryptophane of the enterochromaffincell is increased, and a precursor substance is provided for the synthesis of 5-hydroxytryptamine in brain. Therefore, the bifidobacterium longum subsp. infantis CCFM687 has a broad application value.

The invention discloses lactobacillus paracasei Lc19 and a microbial preparation containing the lactobacillus paracasei Lc19. The lactobacillus paracasei Lc19 is preserved in the China General Microbiological Culture Collection Center, and the preservation number of the lactobacillus paracasei Lc19 is CGMCC NO.17827; the lactobacillus paracasei Lc19 not only has a remarkable curative effect on constipation and colitis, but also can improve various characteristic intestinal related florae of constipation and colitis in a targeted manner, improve the abundance of various beneficial bacteria, reduce the abundance of various harmful bacteria, maintain the balance of the intestinal florae, and greatly improve the capability of recovering the health and diversification of the intestinal florae; the lactobacillus paracasei Lc19 also has the advantages of being convenient to eat, simple in treatment, high in remission rate, free of toxic and side effects and the like.

The invention provides a microbial agent YF, wherein the microbial agent YF comprises four strains. The four strains are Bacillus soniensis B-2, Bacillus paralicheniformis B-26, Bacillus soniensis N-16 and Bacillus pumilus N-17. The four strains are all preserved in the China General Microbiological Culture Collection Center (CGMCC), and the preservation date is March 5,2020, wherein the preservation number of the Bacillus soniensis B-2 is CGMCC NO.19454, the preservation number of the Bacillus paralicheniformis B-26 is CGMCC NO.19449, the preservation number of the Bacillus sorola N-16 is CGMCC NO.19448, and the preservation number of the Bacillus pumilus N-17 is CGMCC NO.19450. The microbial inoculum provided by the invention can effectively prevent the corn stalk rot in saline soil, improve soil quality of the saline soil, and obviously promote growth of corns.

The present invention provides a method for enhancing an immune response in a mammal to facilitate the elimination of a chronic pathology. The method involves the removal of immune system inhibitors such as soluble TNF receptor from the circulation of the mammal, thus, enabling a more vigorous immune response to the pathogenic agent. The removal of immune system inhibitors is accomplished by contacting biological fluids of a mammal with one or more binding partner(s) such as TNFα muteins capable of binding to and, thus, depleting the targeted immune system inhibitor(s) from the biological fluids. Particularly useful in the invention is an absorbent matrix composed of an inert, biocompatible substrate joined covalently to a binding partner, such as a TNFα mutein, capable of specifically binding to a targeted immune system inhibitor such as soluble TNF receptor.

The invention discloses a panda source lactobacillus plantarum and application. The strain was preserved in China Center for Type Culture Collection on May 4, 2016 and the preservation number is CCTCC NO: M2016245. The panda source lactobacillus plantarum provided by the invention can be used for improving antibody levels of IgA, IgM and IgG immune globulins of blood serum; a condition that the percent of lymphocytes is reduced and the percent of neutrophils is increased, caused by infection and induction of enterotoxigenic escherichia coli, is inhibited; expression levels of inflammation related factors IL-1beta, IL-8, IL-6, TLR4 and MyD88mRNA (messenger Ribonucleic Acid) are remarkably down-regulated, and intestinal acute inflammation response caused by the enterotoxigenic escherichia coli is alleviated.

Belonging to the technical field of contaminated soil remediation, the invention particularly relates to an improvement method of extremely acidified mine soil. The method utilizes an improving agentand a microbial agent to change the microbial community structure of soil, especially reduce the relative abundance of iron-sulfur oxidation acid production microorganisms and inhibit metal sulfide from oxidizing and producing acid. The improving agent can be utilized for adsorption, chelation, complexing and immobilization of heavy metal ions and reduction of the biological toxicity, thus reducing the diffusion and leakage of heavy metal ions to surrounding soil or water. The improvement method provided by the invention has the advantages of low investment cost, good acidification control andheavy metal stabilization effect, substantial increase of nutrient content, no destruction of the soil structure, and easy implementation.

The invention discloses sargassum pallidum polysaccharide with effects of resisting oxidation and regulating intestinal flora as well as a preparation method and application of the sargassum pallidumpolysaccharide. The preparation method comprises the steps of hot water extraction, deproteinization, decoloration, alcohol precipitation, dialysis and degradation, wherein the degradation process comprises the steps: adding a sargassum pallidum crude polysaccharide solution with the concentration of 5-8 mg / mL into an H2O2 solution until the volume concentration is 1-5%, placing the mixture in anultrasonic field, carrying out radiation treatment at the ultrasonic frequency of 20-25 kHz and the power of 450-550 W, and performing freeze drying to obtain sargassum pallidum polysaccharide. Compared with sargassum pallidum crude polysaccharide, the sargassum pallidum polysaccharide has the average molecular weight of 130-510 kDa, the particle size of 400-500 nm and low viscosity, can significantly improve the abundance of beneficial bacteria in human intestinal tracts and reduce the abundance of harmful bacteria, has an intestinal microorganism utilization rate improved by about 7%, and can be used as a food additive in functional food for regulating intestinal health.

The invention relates to the technical field of biology, in particular to application of polysaccharide. The invention provides the application of lentinan in preparation of healthcare food capable of improving the abundance of beneficial bacteria and reducing the abundance of harmful bacteria in intestinal florae. An applicant for the invention observes the influence of the lentinan on lipid metabolism in mice through the intestinal florae by inducing obesity type-2 diabetic models of the mice through high-fat diet; the lentinan provided by the invention can regulate intestinal flora disturbance caused by diet, and can obviously change the proportion of the harmful bacteria in the intestinal florae and make unbalanced intestinal florae in vivo to trend to be normal so as to regulate the blood fat and blood sugar indicators of obesity caused by the intestinal flora disturbance due to the diet; the content of triglyceride in blood plasma can be obviously reduced; the content of cholesterol, low-density lipoprotein and free fatty acid can be reduced.

The invention provides a polypeptide probe based on photo-crosslinking and nano-metal particle self-assembly technologies. The polypeptide probe is modified with protein-post-translational modified polypeptide. According to the polypeptide probe, affinity recognition and enrichment capture processes are integrated, so that the convenient and efficient recognition and identification of a protein-post-translational modified binding protein or a compound of the binding protein. According to the polypeptide probe of the invention, a photo-crosslinking group is introduced into nano metal particles,so that a weak acting force between the polypeptide and the binding protein is converted into a strong covalent bond effect formed by the photo-crosslinking group, and therefore, the polypeptide probe can bear strong elution conditions and can capture proteins with low abundance and transient interaction; the influence of the modification of the photo-crosslinking group on a polypeptide frameworkis avoided, and therefore, the original structure and recognition characteristic of the polypeptide framework are well maintained, and influence on the binding specificity of the corresponding binding protein is greatly reduced. The invention further provides a preparation method of the polypeptide probe, and the application of the polypeptide probe to the identification of post-translational-modified binding proteins, the identification of interaction between protein modifications and the discovery of new protein-modified binding proteins.

The present disclosure describes compositions and methods for increasing the abundance of commensal bacteria belonging to the order Clostridiales, including Blautia, Ruminococcus, Clostridium, Eubacterium, Holdemania and Dorea species, that are as-sociated with reduced lethal GVHD and improved overall survival following bone mar-row or hematopoietic stem cell transplant. The present disclosure, therefore, provides methods for reducing the likelihood, incidence or severity of GVHD by (1) avoiding the loss of endogenous beneficial species through antibiotic selection; (2) by administering a therapeutically effective amount of a composition comprising one or more Clostridiales associated with reduced GVHD to individuals who may lack or have lost those strains from their intestinal microbiota. Additionally, support for endogenous or reestablished Clostridiales related to reduced GVHD as a treatment option for reducing GVHD can also be provided in the form of nutritional supplementation, for example, sugars fermented by some species of Clostridiales with GVHD reducing activity.

The invention relates to the technical field of biology, and especially relates to a purpose of ganoderan in preparation of health food for regulating flora in intestinal tract. The invention provides the purpose of ganoderan in preparation of health food capable of increasing beneficial bacteria abundance in flora of intestinal tract and reducing harmful bacterium abundance. The applicant of the invention adopts high-fat feed to induce a mouse obesity type-II diabetes model to observe the influence of ganoderan on lipid metabolism of mice through enteric florae, and by adopting the ganoderan, enteric flora disturbance caused by diet can be adjusted, constitution of enteric florae can be remarkably improved, and the unbalance enteric florae in bodies can be recovered, so that the blood fat and the blood sugar indexes of obesity caused by enteric flora disturbance caused by diet can be adjusted, the content of triglyceride in blood plasma can be remarkably reduced, and the content of cholesterol, low density lipoprotein and free fatty acid can be reduced.

The invention relates to complex probiotic for treating and preventing allergic diseases as well as a preparation method and a preparation of the complex probiotic. The complex probiotic is characterized by being prepared from strain composition shown in the description in percentage by weight.

The invention discloses a La-Mg-Ni series hydrogen storage alloy for a low-temperature nickel-hydrogen battery and a preparation method of the La-Mg-Ni series hydrogen storage alloy. The chemical component of the alloy is La<0.75-x>CexMg0.25Ni3Co0.5, wherein the subscripts in the chemical component are atomic ratios of corresponding constituents, and the value range of x is 0.09-0.11. The preparation method of the alloy comprises the following steps of raw material preparation; smelting and casting; alloy thin strip preparation; and grinding and screening. The alloy prepared by the method hashigh low-temperature electrochemical performance, and under the condition of 248K, at the quenching rate of 10 m.s<-1>, when charging and discharging are circulated 100 times, the discharge capacity of the alloy reaches the maximum value of 245.9 mAh.g<-1>. An alloy material prepared by the preparation method is particularly suitable for being used under the condition of zero DEG C or below, particularly under the condition of minus 25 DEG C and even under the condition of minus 30 DEG C.

The invention relates to bifidobacterium longum subspecies CCFM687, fermented food and application thereof. Bifidobacterium longum infantile subspecies CCFM687 of the present invention can improve the depression-like behavior of depressed mice, improve the levels of 5-hydroxytryptamine, 5-hydroxytryptophan and brain-derived neurotrophic factor in the brain of depressed mice; improve depression Butyric acid content in the gut of mice; lower intestinal abundance of Desulfovibrio spp., increased abundance of Bifidobacterium and S24‑7 families, increased gut microbiota α‑diversity, and improved intestinal Intestinal flora disorder, reduce the occurrence of autism, inflammatory bowel disease, obesity and type I diabetes; increase the mRNA level of tryptophan hydroxylase 1 in simulated enterochromaffin cells, and increase the 5-hydroxychromatin level of the cells The secretion of amino acid provides precursor substances for the synthesis of 5-hydroxytryptamine in the brain. Therefore, it has broad application value.

The invention discloses cordyceps militaris intracellular polysaccharide and a preparation method thereof, and applications of the cordyceps militaris intracellular polysaccharide in regulation of intestinal flora. The method comprises the following steps: carrying out fermentation culture on cordyceps militaris strains, and separating to obtain cordyceps militaris mycelia; degreasing the cordyceps militaris mycelia, and performing hot water leaching to obtain a cordyceps militaris leaching liquid; and removing protein by adopting trypsin and a Sevage method, carrying out ethanol precipitation, and carrying out DEAE-Sepharose Fast Flow column chromatography purification to obtain the cordyceps militaris intracellular polysaccharide. The cordyceps militaris intracellular polysaccharide prepared by the invention can regulate the intestinal bacterial flora structure of a high-fat diet mouse, promote the growth of intestinal probiotics of high-fat diet mice, improve the proportion of phylum firmicutes / bacteroides, adjust the structure of fungal flora, promote the synthetic route of intestinal butyrate, induce activation of short-chain fatty acid receptors, promote fat metabolism and inhibit fat accumulation so as to achieve the effects of reducing blood fat and protecting the liver.

The present invention relates to a new method for using a novel nanometer composite material for protein sample treatment. According to the present invention, with the application of the material in the protein sample pretreatment, the abundance of the high-abundance proteins can be effectively reduced, the complexity of the sample can be reduced, and the more low-abundance proteins in the blood plasma can be identified; and compared with the traditional protein sample pretreatment method, the protein sample pretreatment method of the present invention has characteristics of convenience, easy performing, low cost and the like.

The embodiment of the invention relates to application of a mycotoxin biodegradation agent to promotion of healthy pig production, in particular to application of the mycotoxin biodegradation agent tothe preparation of feed for positively regulating a microbial flora in contents of pig jejunum, improving the detoxification ability of pigs and promoting healthy growth. The mycotoxin biodegradationagent can up-regulate the expression of 153 proteins in the liver of piglets and down-regulate the expression of 55 proteins. The differential proteins are mainly involved in the composition of cellcomponents, molecular functions and biological processes. Analysis of KEGG pathways of the differential proteins shows that the mycotoxin biodegradation agent can up-regulate the expression of 13 proteins without down-regulated proteins, the KEGG pathways in which the up-regulated proteins mainly participate include endoplasmic reticulum protein processing, glycolysis / gluconeogenesis, tryptophan metabolism, valine, leucine and isoleucine metabolism, microbial metabolism in different environments, starch and sucrose metabolism and the like.

The invention discloses application of 2'-fucosyllactose to regulation of host intestinal flora and enhancement of intestinal barriers, and belongs to the technical field of biological medicine. The new application includes an application of the 2'-fucosyllactose to preparation of drugs for regulating the host intestinal flora and enhancing the intestinal barriers, an application of the 2'-fucosyllactose to preparation of health food for regulating the host intestinal flora and enhancing the intestinal barrier, and an application of the 2'-fucosyllactose to preparation of food for regulating the host intestinal flora and enhancing the intestinal barriers. The situation that the 2'-fucosyllactose has effects in regulating the host intestinal flora and enhancing the intestinal barriers, andcan be used for preventing and relieving intestinal flora disorder and intestinal barrier damage caused by pathogenic bacteria infection and the like is disclosed for the first time.

The invention discloses lactobacillus fermentum and an application thereof in improving intestinal health, which belongs to the technical field of microorganisms and medicines. The lactobacillus fermentum CCFM1037 screened by the invention can improve the intestinal health, which is specifically embodied in that (1) the lactobacillus fermentum CCFM1037 can grow by taking fructo-oligosaccharide, xylooligosaccharide and galactooligosaccharide as a unique carbon source and metabolize to produce acid, and has broad-spectrum oligosaccharide utilization capability; (2) various short-chain fatty acids such as acetic acid, propionic acid and butyric acid can be produced through in-vitro metabolism; and (3) the relative abundance of Parabacteroides in intestinal tracts can be obviously reduced, sothat the lactobacillus fermentum CCFM1037 has a huge application prospect in the preparation of products (such as foods, medicines or health care products) for improving the intestinal health.

Production of stable isotope 15N marked L-valine is carried out by selecting proper bacterium, slope preparing, culturing formula by fermentation, fermenting, separating and extracting. It can add micro-vitamin composite, improve growth and metabolism and decrease 15N utilization rate.

The invention provides a vegetation recovery method for extremely acidified mine soil, and belongs to the technical field of vegetation recovery. The method follows a primary succession rule; plant configuration is performed in a layered and staged manner; in the first stage, primary succession started in a primary bare area is simulated, acid-resistant and heavy-metal-resistant plants are taken as main plants, and multiple plants such as trees, shrubs and grasses are subjected to group configuration; and in the second stage, tolerant plants are transited to natural succession through the compensation effect of biodiversity, and a near-natural plant community independent of the tolerant plants is constructed. According to the method, a stable three-dimensional vegetation ecological systemcan be established on the extremely acidified mine soil in a short time, soil acidification and re-acidification are effectively controlled, and the long-term stability of the vegetation system is achieved. The method is easy to operate, rapid in vegetation reconstruction, high in coverage degree, not prone to degradation, good in acidification control effect and suitable for large-area application and popularization.

The invention provides a meat goose feed for improving feed intake in a fattening period. Each 100 kg of feed comprises the following components: 9.5-29.5 kg of corn flour, 8 kg of soybean meal, 20-40kg of broken rice, 20 kg of Qinghua rice, 8 kg of bran, 10 kg of rice bran, 4.5 kg of special additive premix for meat geese and 2.5 kg of special biological fermented feed for meat geese. The feed provided by the invention can reduce the proportion of caecum escherichia coli of the meat geese, reduce the production of serum LPS, reduce the expression of satiety signal, stimulate appetite througha brain gut axis approach, and promote the feed intake of the meat geese. The feed can also promote the intestinal development of the meat geese, improve the villus height of the intestinal tract, and facilitate the intestinal health of the meat geese. On the basis of balanced nutrition, the novel meat goose feed provided by the invention is added with food processing byproducts such as the broken rice and the Qinghua rice in a formula, improves the proportion of fiber feeds, has low cost and good breeding effect, and can be widely applied to the field of meat goose breeding.

The invention belongs to the technical field of immunotherapy, and particularly relates to application of a hypoxia-adjustable promoter in CAR-T, a CAR structure of the hypoxia-adjustable promoter, anexpression vector, CAR-T cells, application of the CAR-T cells, a method for improving the IFN-gamma and / or IL-2 factor secretion capacity of the CAR-T cells in a hypoxia environment, and a method for improving the CAR-T cell killing capacity in the hypoxia environment. The CAR structure contains the hypoxia-adjustable promoter, the hypoxia-adjustable promoter is formed by connecting Hif1a adjusting elements and mini promoters, and the number of repetitions of the Hif1a adjusting elements is 3-5. The CAR structure induced and started by the hypoxia microenvironment not only can be effectivelyexpressed in T lymphocytes, but also can enhance the activation of the CAR-T cells in the hypoxia environment and enhance the effectiveness and safety of the CAR-T, and can be used for targeted therapy of tumors.

The invention discloses a modified apple pectin which has a purity of higher than 90%, a methyl esterification degree of lower than 6%, an acetylation degree of less than 2%, a molecular linearity ofgreater than 12, and an average molecular weight of 8.0-15 kDa, and a degradation rate of higher than 90% after 12 h in the intestinal tract. The invention further discloses a preparation method of the pectin, which comprises the following steps: uniformly mixing water and apple powder; sequentially carrying out enzymolysis by using alpha-amylase and the like to obtain an apple powder enzymatic hydrolysate, carrying out concentration and suction filtration, and performing hot leaching on the filter residue with acid water to obtain a crude pectin extract; firstly adding pectin methylesterase and pectin acetylesterase to the crude pectin extract for enzymolysis, and then adding rhamnogalacturonase and the like thereto for enzymolysis to obtain a pectinase enzymatic hydrolysate; subjecting the pectinase enzymatic hydrolysate to high-pressure dynamic micro-jet treatment before concentration, adding ethanol to precipitate, re-dissolving the precipitate for ultrafiltration and dialyzing, and carrying out concentration and drying to obtain the modified apple pectin. According to the invention, the modified apple pectin has an increased intestinal fermentation speed and a higher degradation rate than commodity apple pectin.

The invention discloses a coating diaphragm with high flame retardance and lithium dendrite growth inhibition and a preparation method thereof.The preparation method comprises the following steps that 1, functionalized BNNS nanosheets and Mg (OH) 2 powder are stirred and dispersed in water and homogenized, and coating slurry is obtained; 2, coating a base membrane with the coating slurry, and drying; a diaphragm A is obtained; step 3; dipping the diaphragm A in a polydopamine solution, washing and drying; and obtaining the coated diaphragm. (1) through the synergistic effect between the functionalized BNNS nanosheet and Mg (OH) 2, the ion transmission rate, the flame retardant property and the heat resistance of the coated diaphragm are remarkably improved; (2) through modification of dopamine and tannic acid, the hydrophilicity of a coating layer is increased, the ion conduction rate and the ion conductivity are greatly improved, and the growth of lithium dendrites in the battery is effectively inhibited; and meanwhile, the adhesion between the functionalized BNNS nanosheet and the Mg (OH) 2 and the base membrane is improved.

The invention discloses a microbial preparation with inactivated streptococcus thermophilus MN002 as well as a preparation method and application of the microbial preparation. The streptococcus thermophilus MN002 is preserved in the China General Microbiological Culture Collection Center (CGMCC) on May 7, 2010, and the preservation number is CGMCC No.3817. The inactivated streptococcus thermophilus MN002 product disclosed by the invention has the effects of improving colitis and characteristic intestinal flora thereof, and improving constipation and characteristic intestinal flora thereof.