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Sargassum pallidum polysaccharide with effects of resisting oxidation and regulating intestinal flora as well as preparation method and application of sargassum pallidum polysaccharide

A technology for regulating intestinal flora and sea wormwood polysaccharides, applied in the direction of food ingredients as antioxidants, application, polysaccharide/gum-containing food ingredients, etc., can solve the problems of high energy consumption, high reaction temperature, long reaction time, etc., to achieve High degradation efficiency, mild reaction conditions, simple and easy-to-operate equipment

Active Publication Date: 2020-06-16
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method can prepare polysaccharides with both antioxidant and hypoglycemic activities, but this method has the disadvantages of long reaction time, high reaction temperature, and high energy consumption.

Method used

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  • Sargassum pallidum polysaccharide with effects of resisting oxidation and regulating intestinal flora as well as preparation method and application of sargassum pallidum polysaccharide
  • Sargassum pallidum polysaccharide with effects of resisting oxidation and regulating intestinal flora as well as preparation method and application of sargassum pallidum polysaccharide
  • Sargassum pallidum polysaccharide with effects of resisting oxidation and regulating intestinal flora as well as preparation method and application of sargassum pallidum polysaccharide

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Effect test

Embodiment 1

[0038]1) After drying the Artemisia annua, crush it, pass through a 60-mesh sieve, mix the Artemisia Artemisiae powder with 95% ethanol at a mass volume ratio of 1g:3mL, add 95% ethanol, heat and reflux for 3 hours at a temperature of 70°C, and centrifuge After 20 minutes, the residue was separated, and the residue was repeated twice, and dried at 45°C for 24 hours;

[0039] 2) Mix the dry powder pretreated in step 1) with water at a mass-to-liquid ratio of 1 g: 20 mL, and extract at a temperature of 80° C. for 2 hours and 2 extraction times; centrifugal separation, Combine the extracts, concentrate under reduced pressure at 50°C to 1 / 4 of the original volume, and obtain the concentrated solution of Artemisia annua polysaccharide;

[0040] 3) Add Sevag reagent to the concentrated solution of Artemisia polysaccharides, the volume ratio of the concentrated solution of Artemisia polysaccharides to Sevag reagent (chloroform:n-butanol=3:1) is 4:1, shake for 30min, and centrifuge to...

Embodiment 2

[0045] 1) After drying the sea wormwood, crush it, pass through a 60-mesh sieve, add 95% ethanol according to the mass volume ratio of the sea wormwood powder to 95% ethanol, heat and reflux for 4 hours at a temperature of 70°C, and centrifuge for 20 minutes , the residue was separated, and the residue was repeated twice for the above operation, and dried at 45°C for 24h;

[0046] 2) Mix the dry powder pretreated in step 1) with water at a mass-volume ratio of 1g:20mL, and extract at a temperature of 80°C for 2 hours and 3 extraction times; centrifugal separation, Combine the extracts, concentrate under reduced pressure at 50°C to 1 / 4 of the original volume, and obtain the concentrated solution of Artemisia annua polysaccharide;

[0047] 3) Add Sevag reagent to the concentrated solution of Artemisia polysaccharides, the volume ratio of the concentrated solution of Artemisia polysaccharides to Sevag reagent (chloroform:n-butanol=3:1) is 4:1, shake for 30min, and centrifuge to r...

Embodiment 3

[0052] 1) After drying the sea wormwood, crush it, pass through a 60-mesh sieve, add 95% ethanol according to the mass volume ratio of sea wormwood powder and 95% ethanol, heat and reflux for 3 hours at a temperature of 70°C, and centrifuge for 20 minutes , the residue was separated, and the residue was repeated twice for the above operation, and dried at 50°C for 24h;

[0053] 2) Mix the pretreated dry powder in step 1) with water at a mass-volume ratio of 1 g: 20 mL, and extract at a temperature of 80° C. for 2 hours and 4 extraction times; centrifugal separation, Combine the extracts, concentrate under reduced pressure at 50°C to 1 / 10 of the original volume, and obtain the concentrated solution of Artemisia annua polysaccharide;

[0054] 3) Add Sevag reagent to the concentrated solution of Artemisia polysaccharides, the volume ratio of the concentrated solution of Artemisia polysaccharides to Sevag reagent (chloroform:n-butanol=3:1) is 4:1, shake for 30min, and centrifuge t...

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PUM

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Abstract

The invention discloses sargassum pallidum polysaccharide with effects of resisting oxidation and regulating intestinal flora as well as a preparation method and application of the sargassum pallidumpolysaccharide. The preparation method comprises the steps of hot water extraction, deproteinization, decoloration, alcohol precipitation, dialysis and degradation, wherein the degradation process comprises the steps: adding a sargassum pallidum crude polysaccharide solution with the concentration of 5-8 mg / mL into an H2O2 solution until the volume concentration is 1-5%, placing the mixture in anultrasonic field, carrying out radiation treatment at the ultrasonic frequency of 20-25 kHz and the power of 450-550 W, and performing freeze drying to obtain sargassum pallidum polysaccharide. Compared with sargassum pallidum crude polysaccharide, the sargassum pallidum polysaccharide has the average molecular weight of 130-510 kDa, the particle size of 400-500 nm and low viscosity, can significantly improve the abundance of beneficial bacteria in human intestinal tracts and reduce the abundance of harmful bacteria, has an intestinal microorganism utilization rate improved by about 7%, and can be used as a food additive in functional food for regulating intestinal health.

Description

technical field [0001] The invention relates to the field of polysaccharides, in particular to a sea wormwood polysaccharide with the functions of anti-oxidation and regulating intestinal flora, its preparation method and application. [0002] technical background [0003] Artemisia annua, also known as "big-leaf seaweed", belongs to the Phaeiophyta Sargassum family and is widely distributed in warm temperate waters. Haihaozi is widely distributed in the Bohai Sea, Yellow Sea, East China Sea and other regions of China. It is dark brown and is a typical marine brown algae. It has a bitter, salty and cold taste and enters the spleen, kidney and lung. Functions and indications Cure diuresis, expel heat, painful nuclei, chronic bronchitis, etc., and have high medicinal value. Sea Artemisia contains a large amount of saturated and unsaturated fatty acids, and the content of unsaturated fatty acids is much higher than that of saturated fatty acids. Sea Artemisia is rich in variou...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A23L33/125A61P1/00A61P39/06
CPCC08B37/006C08B37/0003A61P39/06A61P1/00A23L33/125A23V2002/00A23V2200/02A23V2200/3202A23V2250/50
Inventor 袁旦李超扶雄
Owner SOUTH CHINA UNIV OF TECH
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