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Application of lentinan in preparation of healthcare food with intestinal flora regulating function

A technology to regulate intestinal flora and lentinan, applied in food science, medical raw materials derived from fungi, digestive system, etc., can solve problems such as no metagenomic research reports on lentinan

Inactive Publication Date: 2017-05-10
INFINITUS (CHINA) CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It has the functions of anti-inflammation, anti-virus, anti-tumor, regulating immune function and stimulating the formation of interferon, but there is no metagenomics research report on which specific beneficial and harmful bacteria can be regulated by lentinan in the intestinal flora

Method used

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  • Application of lentinan in preparation of healthcare food with intestinal flora regulating function
  • Application of lentinan in preparation of healthcare food with intestinal flora regulating function
  • Application of lentinan in preparation of healthcare food with intestinal flora regulating function

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] The lentinan of the present invention is prepared by the following preparation method: After the shiitake mushrooms are crushed, water is added according to the ratio of shiitake mushrooms: water = 1g: 5mL, heated and stirred at 75°C for 3 hours, and the filter residue obtained by filtration is prepared according to the above-mentioned ratio of material to liquid and temperature Continue to extract twice with time, combine three extractions, concentrate under reduced pressure and centrifuge to obtain a clarified concentrate, add 9 times the volume of 95% ethanol to the clarified concentrate according to the volume ratio of the clarified concentrate: 95% ethanol=1:9 After mixing, let stand overnight at 4°C to precipitate, centrifuge to collect the precipitate, and freeze-dry in a vacuum to obtain lentinan. The lentinan of the present invention can also be purchased in the market.

Embodiment 2

[0048] The applicant of the present invention has carried out a pharmacological experiment using the lentinan prepared in Example 1, and the experimental results are as follows:

[0049] 1. Experimental materials

[0050] 1.1. Experimental Instruments

[0051] Thermo full-wavelength microplate reader: Thermo Fisher Scientific China, 1510;

[0052] JA1203N analytical balance: product of Shanghai Precision Instrument Co., Ltd.;

[0053] Electronic balance: Changshu Shuangjie Testing Instrument Factory, T1000.

[0054] 1.2. Experimental reagents

[0055] Glucose determination kit: Shanghai Rongsheng Biopharmaceutical Co., Ltd., 20131207147;

[0056] Total cholesterol assay kit: Shanghai Rongsheng Biopharmaceutical Co., Ltd., 20131105146;

[0057] Triglyceride determination kit: Shanghai Rongsheng Biopharmaceutical Co., Ltd., 20131105121;

[0058] Low-density lipoprotein assay kit: Shanghai Rongsheng Biopharmaceutical Co., Ltd., 20140302124;

[0059] Free fatty acid quantit...

Embodiment 3

[0102] The fresh feces of the mice in the 12th week of the mouse experiment in Application Example 1 were collected, and the intestinal flora among different groups were analyzed for differences. figure 1 And B, positive drug group in table 6, C, normal group, D, lentinan group 1, E, lentinan group 2, F, lentinan group 3, M, model group (grouping is the same as table 2 in embodiment 2) .

[0103] This research is most concerned about whether the bacteria with differences between the M model group and the C normal group can be adjusted after medication, and the figure 1 . figure 1 The OTUs selected in are the OTUs with differences between the C normal group and the M model group (their effectsize>1.96), and then these OTUs are also tested between other groups and the M model group, and the obtained effectsize draws the above heat map . from figure 1 We can easily see that the effect of the positive drug is very significant, and the number of bacteria enriched in the M model...

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Abstract

The invention relates to the technical field of biology, in particular to application of polysaccharide. The invention provides the application of lentinan in preparation of healthcare food capable of improving the abundance of beneficial bacteria and reducing the abundance of harmful bacteria in intestinal florae. An applicant for the invention observes the influence of the lentinan on lipid metabolism in mice through the intestinal florae by inducing obesity type-2 diabetic models of the mice through high-fat diet; the lentinan provided by the invention can regulate intestinal flora disturbance caused by diet, and can obviously change the proportion of the harmful bacteria in the intestinal florae and make unbalanced intestinal florae in vivo to trend to be normal so as to regulate the blood fat and blood sugar indicators of obesity caused by the intestinal flora disturbance due to the diet; the content of triglyceride in blood plasma can be obviously reduced; the content of cholesterol, low-density lipoprotein and free fatty acid can be reduced.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the use of lentinan in preparing health food with the effect of regulating intestinal flora. Background technique [0002] People's eating habits mostly prefer high-fat, high-sugar and high-calorie diets. With the improvement of material living standards, people's demand for high-fat diets is also increasing. A high-fat diet is also one of the important causes of diseases such as obesity and diabetes. Studies in recent years have found that some polysaccharide-rich foods and medicines have better effects in reducing blood sugar and lipids. Studies have shown that gut flora can control fat metabolism, trigger systemic chronic low-grade inflammation, and induce obesity and insulin resistance. Diet structure is an important factor in determining the composition of intestinal flora. Maintaining intestinal microecological balance is necessary to maintain human health. [0003] Most of...

Claims

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Application Information

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IPC IPC(8): A23L33/125A61K36/06A61K31/715A61P1/00A61P3/04A61P3/00
CPCA61K31/715A61K36/06A23V2002/00A23V2200/32
Inventor 马方励马忠华胡明华王园园葛亚中金鑫
Owner INFINITUS (CHINA) CO LTD
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