Circular system for cultivating abundant isotope carbon and nitrogen double-labeling plant sample

Abstract

The invention relates to the technical field of the isotope labeling and in particular to a circular system for a cultivating abundant isotope carbon and nitrogen double-labeling plant sample. The circular system for the cultivating abundant isotope carbon and nitrogen double-labeling plant sample comprises a sealed cultivation chamber, a CO2 injector, a gas balancing device, a nutrient solution injector and a nutrient solution circular system. The sealed cultivation chamber comprises a transparent cover and a base. The transparent cover is internally provided with a semiconductor condensation dehumidification device. The transparent cover is connected with the CO2 injector and the gas balancing device. A cultivation basin, a liquid storage chamber, an external peristaltic pump, an ultraviolet sterilizing device, a circular peristaltic pump and a converging pool are successively connected to form a circular loop of the nutrient solution circular system. The converging pool is connected with the semiconductor condensation dehumidification device and the nutrient solution injector. The cultivation basin and the liquid storage chamber are installed in the base. The top of the cultivation basin is communicated with the transparent cover. Silica sand is installed in the cultivation basin. The circular system for the cultivating abundant isotope carbon and nitrogen double-labeling plant sample is capable of simultaneously labeling the carbon and nitrogen isotope, and realizing the cyclic utilization. The pollution of normal 12 C and 14 N is avoided, and an abundant carbon and nitrogen isotope double-labeling plant can be obtained.

Description

technical field [0001] The invention relates to the technical field of isotope labeling, in particular to a circulation system for cultivating high-abundance isotope carbon and nitrogen double-labeled plant samples. Background technique [0002] In recent years, the cycle process of carbon and nitrogen contained in crop straws in soil has become one of the research hotspots in plant nutrition and soil science. Isotope tracer technology is a key technology to study the decomposition and transformation process of crop straw in soil, which can effectively reveal the release law of straw elements and the bioavailability of organic nutrients. Stable isotope nitrogen ( 15 N) labeled crop stalks were applied to the soil, and by measuring the different components of the soil 15 The change of N abundance can accurately calculate the straw 15 The amount of N nutrient transferred to soil and plants, and the proportion lost through gas, leaching, etc. Similarly, using the stable iso...

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Problems solved by technology

[0008] The invention provides a circulation system for cultivating high-abundance isotope carbon and nitrogen double-labeled plant samples, which is used to solve the problem that the existing isotope labeling technology cannot realize the circulation culture of isotope carbon and nitrogen double-labeling, and cannot obtain high-abundance double-labeled plants sample problem

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