The invention relates to the technical field of the
isotope labeling and in particular to a circular
system for a cultivating abundant
isotope carbon and
nitrogen double-labeling
plant sample. The circular
system for the cultivating abundant
isotope carbon and
nitrogen double-labeling
plant sample comprises a sealed cultivation chamber, a CO2
injector, a gas balancing device, a
nutrient solution
injector and a
nutrient solution circular
system. The sealed cultivation chamber comprises a transparent cover and a base. The transparent cover is internally provided with a
semiconductor condensation dehumidification device. The transparent cover is connected with the CO2
injector and the gas balancing device. A cultivation basin, a
liquid storage chamber, an external
peristaltic pump, an
ultraviolet sterilizing device, a circular
peristaltic pump and a converging
pool are successively connected to form a
circular loop of the
nutrient solution circular system. The converging
pool is connected with the
semiconductor condensation dehumidification device and the
nutrient solution injector. The cultivation basin and the
liquid storage chamber are installed in the base. The top of the cultivation basin is communicated with the transparent cover. Silica sand is installed in the cultivation basin. The circular system for the cultivating abundant isotope carbon and
nitrogen double-labeling
plant sample is capable of simultaneously labeling the carbon and nitrogen isotope, and realizing the cyclic utilization. The
pollution of normal 12 C and 14 N is avoided, and an abundant carbon and nitrogen isotope double-labeling plant can be obtained.