The invention discloses a
sugar-free
tissue culture system for industrialization of rare
medicinal plants, and relates to the technical field of
plant tissue culture and intelligent
bioreactor control. Comprising a temporary immersion culture unit, a
carbon dioxide supply and uniform mixing unit, a
liquid phase buffer adding unit, an environment regulation and
control unit, a physiological state acquisition unit, a multi-point
carbon dioxide sensor array, an edge controller, a special risk constraint
large model controller and a block chain-based credible strategy consistency network. According to the
system, a
sugar-free culture medium is used as a culture solution, a temporary immersion
rhythm formed by an immersion phase and a ventilation phase is adopted, a carbon supply window is set at the ventilation phase, and
carbon dioxide injection is forbidden at the immersion phase; a uniformity threshold value, a
rise time threshold value, a deviation correction and amplitude limiting rule, a carbon supply curve parameter set and a
liquid phase buffer parameter set bound with the carbon supply curve parameter set are generated in the
large model, and a risk upper bound quantile and a strategy admission threshold value are calculated based on a rolling
calibration set to achieve admission; the strategy packet is executed after being subjected to block chain
consensus release and edge
verification, correction, amplitude limiting and degradation are triggered in a window according to criteria, and a
calibration set is written back, so that cross-base consistency triggering calibration and
recovery are realized.