Technology for rapid sugar-free micropropagation of dendrobium officinale kimura et migo under non-aseptic condition
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A non-sterile condition, technology of Dendrobium candidum, applied in horticulture, botany equipment and methods, gardening tools/equipment, etc., can solve the problems of high cost and long tissue culture cycle
Inactive Publication Date: 2012-12-26
荆树汉
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[0005] The purpose of the present invention is to provide a kind of reproduction technology of Dendrobium candidum under non-sterile conditions. The method is low in cost, easy to operate, saves energy and labor costs, and better solves the problem of high cost in the current tissue culture process of Dendrobium candidum. The problem of long training period
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Embodiment 1
[0030] Further illustrate substantive content of the present invention with embodiment below. But the content of the present invention is not limited thereto.
[0031] The surface of Dendrobium candidum capsules collected from Guiping, Guangxi was sterilized to obtain aseptic seeds, and the seeds were sown on the aseptic germination solid medium. The medium composition was 1 / 2MS, added 2% sucrose, 20% potato extract, and boiled for 20 minutes. Stir it into milk and add it to the medium, the hormone is 0.5mg / L, the agar is 0.5%, and the pH value is 6.2. The culture bottle is placed at a temperature of 20-25° C., and the light is natural light or fluorescent lamp 1500 Lux, and cultured for 2 months.
[0032] Induction and propagation of germinated protocorms
[0033] The method of induction and propagation is 1 / 2MS, 15% potato, 3% sucrose, boiled for half an hour, stirred into milk and added to the medium, plus NAA0.5mg / L, 6BA0.5mg / L, activated carbon 0.2%, agar 0.6%, cultiva...
Embodiment 2
[0038] The surface of dendrobium candidum capsules collected by Zhejiang Leqin was sterilized to obtain sterile seeds. The seeds were sown on the sterile germination solid medium. The medium composition was 1 / 2MS, adding 2% sucrose and 20% potato extract, and boiled for 20 minutes Stir it into milk and add it to the medium, the hormone is 0.5mg / L, the agar is 0.5%, and the pH value is 6.2. The culture bottle is placed at a temperature of 20-25° C., and the light is natural light or fluorescent lamp 1500 Lux, and cultured for 2 months.
[0039] Induction and propagation of germinated protocorms
[0040] The method of induction and propagation is 1 / 2MS, 15% potato, 3% sucrose, boiled for half an hour, stirred into milk and added to the medium, plus NAA0.1mg / L, 6BA1.0mg / L, activated carbon 0.2%, agar 0.6%, cultivated in the above medium for one month.
[0041] The aseptic seedlings propagated above can be transplanted for rooting culture, the medium is MS, banana extract 15%, s...
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Abstract
The invention discloses a novel rapid propagation technology in the technical field of biology, and relates to a method for the rapid propagation of dendrobium officinale kimura et migo under a non-aseptic condition. The method mainly comprises the steps of: non-aseptic germination of dendrobium officinale kimura et migo seeds, formation of protocorms and seedlings and successive rapid propagation under the non-aseptic condition. Coarse pine bark powder is added into a 1 / 2 MS culture medium to obtain a substrate free of sugar and agar, and the seedlings of the dendrobium officinale kimura et migo are placed into a closed container after being planted, are autotrophically grown through carbon dioxide under an approximately natural condition, and are promoted to be rooted, grown and strengthened within a shorter time. The method has the characteristics that seedling culture time is shortened, high-pressure sterilization is not required during successive culture, the seedlings can be propagated and grown in a normal state, and are difficult to pollute, and the cultured seedlings have large leaves and thick stems, and are high in quality. Compared with the conventional tissue culturing method for the dendrobium officinale kimura et migo, the method has the advantages that energy consumption is reduced, labor cost is reduced, and the method has the three major advantages of high speed, energy saving and good effect.
Description
technical field [0001] The invention is a rapid propagation technology in the field of biotechnology, and relates to a method for rapid propagation of famous and precious medicinal material Dendrobium officinale under non-sterile conditions. technical background [0002] Dendrobium is a commonly used traditional Chinese medicine in my country. The 2010 edition of the Chinese Pharmacopoeia has listed Dendrobium officinale as a single species collection, so it presents a unique curative effect that its efficacy or value is more precious than ordinary Dendrobium. It has the effects of nourishing the stomach and promoting body fluid, moistening the lungs and relieving cough, etc. It is often used for various diseases such as fever injury, dry mouth and polydipsia, and post-illness deficiency heat. Due to the destruction of forest ecology and excessive exploitation of resources, wild resources are on the verge of exhaustion. In 1987, the State Council listed it as a national key p...
Claims
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