The application discloses a
jellyfish saussurea extract with
acetylcholinesterase inhibitory activity and a preparation method thereof, and relates to the technical fields of
traditional Chinese medicine extraction and nerve drugs. The
jellyfish saussurea extract is prepared by the following steps:
drying jellyfish saussurea whole grass, crushing, sieving, obtaining jellyfish saussurea coarse
powder, adding an
acetic acid-
sodium acetate
buffer solution with a pH value of 4.5-5.0 to the coarse
powder, and the adding amount of the
buffer solution is 1:10-1:20 (w / v). A composite
enzyme is added to the above-mentioned mixed
system, and the composite
enzyme is composed of
cellulase and
pectinase, and the
mass ratio of the two is 1.5-2.5:1. The application adopts the integration of "composite enzymatic wall breaking, double-frequency
ultrasonic assisted extraction, and
macroporous resin directional
elution", and the AChE inhibitory active ingredients (
small molecule phenolic acid and
flavonoid glycoside) in the jellyfish saussurea are directionally enriched according to the physical and chemical properties. The composite enzymatic wall breaking (
cellulase+
pectinase) can efficiently degrade
plant cell wall
cellulose and intercellular layer
pectin, and significantly improve the wall breaking rate.