The invention provides a fresh ossein seasoning, which is prepared from 100 weight parts of fresh livestock and poultry bone meat and/or frozen livestock and poultry bone meat, 0.5 to 1 weight part of compound protease, 1 to 10 weight parts of edible salt, 0 to 10 weight parts of auxiliary materials and 0 to 10 weight parts of food additives, wherein the fresh livestock and poultry bone meat and/or frozen livestock and poultry bone meat comprises at least one kind of materials from pig bone, pork, ox bone, beef, goat bone, mutton, donkey meat, donkey bone, chicken bone, chicken meat, duct bone and duct meat; the auxiliary materials comprise at least one kind of materials from monosodium glutamate, maltodextrin, edible glucose, white granulated sugar, edible corn starch and composite spice; the food additives comprise at least one kind of materials from flavor development disodium nucleotide, disodium succinate, potassium sorbate, nisin, sodium dehydroacetate, sodium diacetate, octenyl succinate anhydrate starch, acetylated distarch phosphate, hydroxy propyl distarch phosphate and food flavor.