The invention discloses an environmental-friendly mixed culture fish feed capable of strengthening food calling properties. The environmental-friendly mixed culture fish feed is prepared from the following raw materials in percentage by weight: 10-15% of soybean meal, 15-30% of rape seed cakes, 5-15% of cottonseed meal, 2-10% of corn alcohol meal, 2-8% of pig internal organ powder, 2-8% of fish meal, 2-5% of beer yeast powder, 5-15% of high-gluten flour, 1-4% of soybean oil, 0.5-1% of soybean phospholipid oil, 2-5% of calcium dihydrogen phosphate, 2-5% of bentonite, 0.5-1% of bacillus subtilis, 0.2-0.5% of taurine, 0.3-0.6% of dried orange peel, 0.05-0.1% of sulfobetaine, 0.1-0.5% of choline chloride, 0.05-0.2% of methionine, 0.2-0.5% of lysine, 0.2-0.6% of composite trace elements and 0.3-0.4% of composite vitamins. The feed can improve the food calling properties and the digestibility, reduce the powder, and reduce the waste of feeds.