136 results about "Specific substrate" patented technology

Enzyme substrates and associated technology of the present invention are provided. An enzyme substrate of the invention may comprise a biologically functional fluorescent dye and an enzyme-specific substrate moiety attached in such a way that the functionality of the functional dye is diminished. An enzymatic reaction may cleave at least a portion of the substrate moiety from the enzyme substrate to provide a more functional product dye. This product dye may be nonfluorescent or weakly fluorescent, in general, and relatively fluorescent, in a particular condition, such as when bound to a partner biological molecule or an assembly of partner biological molecules. An enzyme substrate of the present invention may thus be useful in fluorescence detection, and / or in any of a variety of useful applications, such as the detection of enzymatic activity in a cell-free system or in a living cell, the screening of drugs, or the diagnosis of disease.

The invention discloses an electrochromic device, and in particular discloses a multi-layer structure of which a main body is transparent glass. A porous aerogel layer is packaged, conductive materials are coated on a specific substrate, and an electrochromic material is packaged among the conductive materials; voltage is applied to the conductive material layer, so that the characteristics of the electrochromic material can be changed; and moreover, photocatalyst materials can be coated on the outer side of the substrate, and a solar power supply layer is arranged, so that power can be supplied to the electrochromic device or the power can be supplied to other devices. According to another embodiment, an electrochromic composite material layer can be packaged between two adjacent substrates in the electrochromic device and has a composite structure consisting of aerogel and the electrochromic material.

The invention discloses a fluorescent probe for detecting the content of glutathione (GSH) in blood, and a synthesis method and an application thereof. The synthesis steps of the probe are simple, and the light stability is good. In the detection process, the fluorescent probe firstly as a substrate is combined with glutathione S-transferase (GST), and undergoes a reaction with another specific substrate GSH of the GST to produce fluorescence, the fluorescence is enhanced by about 35 times, and the maximum emission wavelength is at the near infrared region (beyond 700 nm). Compared with conventional GSH fluorescent probes, the probe can specifically detect the content of the GSH in a complex system, and has the advantages of wide dynamic response window, high efficiency and the like. The probe realizes the specific and high-efficiency identification on the GSH in the complex environment, and has extremely important application value in the biological and medical fields.

Thin films of single crystal-like materials are made by using flow-through ion beam deposition during specific substrate rotation around an axis in a clocking action. The substrate is quickly rotated to a selected deposition position, paused in the deposition position for ionized material to be deposited, then quickly rotated to the next selected deposition position. The clocking motion can be achieved by use of a lobed cam on the spindle with which the substrate is rotated or by stopping and starting a stepper motor at long and short intervals. Other symmetries can be programmed into the process, allowing virtually any oriented inorganic crystal to be grown on the substrate surface.

The invention discloses a method for preparing a perylenequinone pigment under the catalysis of Shiraia bambusicola and belongs to the technical field of bioengineering. In the method, naphthoquinone, cinnamic acid and the like which form a substrate are used to prepare the perylenequinone pigment under the catalysis of the Shiraia bambusicola. The catalytic process is implemented by: A), adding the substrate into culture system in a cell liquid culture process, wherein the perylenequinone pigment yield is up to 2.9g / L and the transformation rate is up to 9.7 percent; or B), placing cells obtained by liquid fermentation culture in a reaction system having the substrate, wherein the perylenequinone pigment yield is up to 35.3g / L and the transformation rate is up to 39.7 percent; or C), adding the substrate into a culture system in a cell solid culture process, wherein the perylenequinone pigment yield is up to 1.5g / 100g and the transformation rate is up to 46 percent. The method adopts a perylenequinone pigment-producing Shiraia bambusicola strain and specific substrate transformation to produce hypocrellin, is high in transformation rate, low in cost, less in step and applicable to industrial production and has excellent industrial application prospect.

A silicon wafer having no epitaxially deposited layer or layer produced by joining to the silicon wafer, with a nitrogen concentration of 1·1013-8·1014 atoms / cm3, an oxygen concentration of 5.2·1017-7.5·1017 atoms / cm3, a central thickness BMD density of 3·108-2·1010 cm−3, a cumulative length of linear slippages ≦3 cm and a cumulative area of areal slippage regions ≦7 cm2, the front surface having <45 nitrogen-induced defects of >0.13 μm LSE in the DNN channel, a layer at least 5 μm thick, in which ≦1·104 COPs / cm3 with a size of ≧0.09 μm occur, and a BMD-free layer ≧5 μm thick. Such wafers may be produced by heat treating the silicon wafer, resting on a substrate holder, a specific substrate holder used depending on the wafer doping. For each holder, maximum heating rates are selected to avoid formation of slippages.

The present invention relates to a method for purifying, modifying and immobilizing recombinant protein. The method utilizes genetic engineering to tag a DNA sequence encoding a target protein with a specific tag and express the vector to obtain a recombinant protein. The recombinant protein is then purified and modified by an affinity column and modification reagent. After exchanging the recombinant protein with a decoupling reagent, the recombinant protein is immobilized onto a specific substrate. The method, combining steps of purification, modification and immobilization, provides convenience to using recombinant protein. The omission of the use of dialysis or molecular sieve leads to a shorter period dedicating for removing excessive reagents and the increase of efficiency of recombinant protein recovery.

A spinner apparatus for manufacturing a photomask, performing a developing process for forming a resist pattern on a specific substrate, and performing an etching process in which a resist pattern is used as an etching mask are provided. A plurality of supply nozzles for supplying a developing solution or an etching solution are provided above the substrate on which processes will be performed and processing conditions such as the temperature and flux of the chemicals supplied from each supply nozzle are independently controlled. Accordingly, it is possible to control the deviation of the critical dimensions of the device.

The process according to the invention for the microbial production of a specific product and methane comprises the following steps: a) the production in a bioreactor (2, 10) of a specific product from a specific substrate using a specific organism, the producer, wherein during the production of this product metabolites such as e.g. low-molecular alcohols, aldehydes and acids, hydrogen and CO2, which may inhibit the production of the product, are obtained; and b) the production of methane by means of a methanogen, wherein in the production of the methane at least one metabolite is decomposed and by this means removed from the bioreactor (2, 10) and its energy is made usable.

The invention discloses a method for preparing a diamond-graphene composite film and relates to a method for preparing a diamond-graphene composite film by adopting a chemical vapor deposition method, belonging to the technical field of novel inorganic functional material preparation. The method comprises the following steps: 1) carrying out special treatment on the surface of a heterogeneous substrate to obtain specific substrate surface micro-pit having high surface energy and high density so as to induce graphene to grow; 2) placing the substrate on the substrate in a vacuum deposition chamber of a direct current plasma jetting growth system to grow a single-layer or minority-layer graphene film; 3) and controlling growth parameter conditions to achieve the self-coordination of a carbon bond atom structure of graphene on the surface layer, thus a diamond microstructure is evolved for growing a diamond film and the diamond-graphene composite film is prepared. The preparation method has simple and reliable process, high efficiency and no pollution, is beneficial to environmental protection and is suitable for industrial production.

The invention relates to a method for preparing duck oil diglyceride through catalysis of immobilized lipase. The method for preparing the duck oil diglyceride through the catalysis of the immobilized lipase comprises the following steps: firstly utilizing an alkali hydrolysis method to prepare fatty acid mixture, mixing the fatty acid mixture with glycerinum, and generating a catalytic reaction by utilizing the immobilized lipase; by utilizing an experiment of four single factors, including enzyme dosage, specific substrate, enzymolysis temperature and enzymolysis time, and response surface analysis method, optimal conditions for preparing the duck oil diglyceride from the immobilized lipase are obtained. When the duck oil diglyceride is prepared from the immobilized lipase, enzyme and product diglyceride can be completely separated, so that no enzyme residue exists in the product, the purity of the product is high, and the safety of foods is guaranteed. Furthermore, the selected immobilized lipase can be recycled and reutilized, so that the production costs are reduced, and environmental pollution is reduced. The method for preparing the duck oil diglyceride, which is provided by the invention, creates a new way for comprehensive utilization of duck oil side-products, the additional value of the duck oil can be largely increased, and the method is a green creative project with the important development value.

The present invention relates to specific substrates for a von Willebrand factor cleaving enzyme, ADAMTS-13, as well as to diagnosis of ADAMTS-13 deficient patients, diagnostic compositions, and kits employing the substrates. Particularly preferable substrate polypeptides for ADAMTS-13 are the polypeptide which begins at amino acid 1587 and ends at amino acid 1668 of SEQ ID NO: 1 in the Sequence Listing, and the polypeptide which begins at amino acid 1596 and ends at amino acid 1668 of SEQ ID NO: 1 in the Sequence Listing. These substrate polypeptides for ADAMTS-13 have high substrate specificity and also superior quantitativeness, and a suitable size for production by recombinant methods.