Modified wound dressings

a technology for wounds and dressings, applied in the field of wound healing, can solve the problems of reducing the quality of life, requiring a lot of care, and chronic and ‘infected’ wounds are typically difficult to heal, so as to improve the sensitivity, precision and specificity of infected wound detection, and improve the detection speed of infected wounds. the effect of exiting assays

Inactive Publication Date: 2019-10-10
CONVATEC TECH INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Benefits of technology

[0011]The technology disclosed herein provides for compositions and methods of detecting infected and/or chronic wounds. The disclosed technology improves upon exiting assays by: increasing the sensitivity, precision and specificity of detection of infected wounds; providing for the ability of qualitative and quantitative measurements; and, increasing the speed of detection of infected wounds in situ and in real-time. The assays and methods described herein are partly based on the use of specific reagents that detect biomarkers and/or probes which are present in infected or chronic wounds. The detection process may involve use of reagents that are specific to the markers present in infected wounds but not non-infected or non-chronic wounds and the detection step may involve qualitative or quantitative measurements of the signal(s) that are generated when the probe is acted upon by the marker. In embodiments wherein the detection method involves detection of enzym

Problems solved by technology

Unfortunately, chronic and ‘infected” wounds are typically hard to heal.
Patients with chronic wounds require a great deal of care and the wound often leads to a reduction in quality of life; a chronic wound can become a problem that some patients must deal with throughout the rest of their life.
Patient co-morbidities can also have a significant effect on the wound healing process, limiting and even halting the process as well as being contributing factors to the loss of quality of life.
Infection of wounds by bacteria delays the healing process, since bacteria produce enzymes and toxins and also compete for nutrients and oxygen with macrophages and fibroblasts whose activities are essential for the healing of the wound.
The inflammatory phase is particularly important to the wound healing process, wherein biochemical reactions at the wound situs facilitate healing but also cause tissue breakdown due to production of excess proteases.
Although proteases play an important role in breaking down dead tissue, in excess, they also have a detrimental effect on viable tissue, cause additional

Method used

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  • Modified wound dressings
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  • Modified wound dressings

Examples

Experimental program
Comparison scheme
Effect test

example 1

on of Polymer 1 (Using CMC Fiber)

[0288]

[0289]Sodium carboxymethylcellulose (NaCMC) fiber (443 mg, 1.08 mmol) was dissolved in deionized water (44 ml) to give a 1% solution. Dowex 650C monosphere ion exchange resin was added in order to provide the acidified CMC (CMC-H). The monospheres were removed by filtration and then tetrabutylammonium hydroxide (TBAH) 40% (aq) was added until the pH was 8-9. The resulting solution was stirred for 30 min before being lyophilized overnight. The lyophilized material (0.38 g) was dissolved in 40 ml dry DMF under nitrogen with stirring and gentle heating over a period of approximately 1 h. The resulting solution was cloudy and off-white. The solution was cooled to approximately 4° C. To this stirred solution, 2-chloro-N-methylpyridinium iodide (CMP-I) (0.33 g, 1.3 mmol) was added. Shortly after this, compound 1-b (0.5 g, 2 mmol) was also added along with 3 drops of dry DCM and a few drops of dry triethylamine. The reaction mixture was kept at 4° C. ...

example 2

on of Polymer 2

[0294]

[0295]A solution of Fmoc-Phe-OH (0.0267 g, 0.069 mmol), HBTU (0.0262 g, 0.069 mmol), DIPEA (0.015 ml, 0.090 mmol) and dry DMF (0.82 ml) was prepared. A 15-ml plastic separating column was set up with a 10 m polyethylene frit and a luer tip was attached. Polymer 1 (0.034 g, 0.097 mmol) was added to the column followed by the amino acid solution. A lid was placed onto the column and was further sealed with parafilm. The column was placed on a blood rotor at RT overnight. The solution was drained using reduced pressure and the solid product was washed with EtOH to provide Polymer 2 as a white solid. Weight=0.0266 g. A Kaiser test was used to confirm absence of terminal amines. FTIR: 3324 (N—H / O—H), 3281 (O—H), 2898 (C—H), 1720 (aromatic C═O), 1666 (C═O urethane stretch), 1660, (C═O amide stretch of Fmoc).

[0296]Solubility of Polymer 2 was determined in a similar manner as for compound 1-c. Data is shown below: x indicates insoluble material.

AfterAfterSolventInitialh...

example 3

on of Polymer 3

[0297]

[0298]Polymer 2 was placed in a solution of piperidine: DMF (1:4 v / v, 6 ml) for approximately 2 h. The resulting de-protected product was then washed with EtOH before conducting a Kaiser test to confirm presence of free amine. This de-protected product (0.080 g, 0.15 mmol) was added to a 15-ml plastic separating column set up with a 10 μm polyethylene frit and a luer tip. A solution of Fmoc-Phe-OH (0.063 g, 0.16 mmol), HBTU (0.062 g, 0.16 mmol), DIPEA (0.23 ml, 1.8 mmol) and dry DMF (6 ml) was prepared and added to the column. A lid was placed onto the column and was further sealed with parafilm. The column was placed on a blood rotor at RT overnight. The solution was drained using reduced pressure and the solid product was washed with EtOH (10 ml×3) to provide the coupling product as a red solid. Weight=0.0691 g. A Kaiser test was used to confirm absence of terminal amines. The coupling product was washed with DCM. FTIR: 3315 (N—H / O—H), 2866 (C—H), 1730 (aromat...

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Abstract

Embodiments described herein relate to compounds for the detection of wounds, e.g., chronic wounds or infected wounds, including compositions, substrates, kits, dressing materials, and articles, and systems containing such compounds. Further embodiments relate to methods of using these compositions, kits and systems in diagnostic assays, and in the diagnosis and/or detection of chronic or infected wounds based on enzymatic conversion of specific substrates which are contained in the compositions. Additional embodiments relate to methods of characterizing wounds based on expression of a plurality of markers and using such information to treat, manage, and follow-up patients suffering from chronic or infected wounds.

Description

CROSS-REFERENCE TO RELATED APPLICATIONS[0001]This application is the U.S. National Phase entry of International Application No. PCT / US2017 / 024915, filed on Mar. 30, 2017, which claims the benefit of U.S. Provisional Application No. 62 / 315,567, filed Mar. 30, 2016, each of which are incorporated herein by reference in their entireties.TECHNICAL FIELD[0002]Embodiments described herein generally relate to wound healing, and in particular to compositions and methods for the detection and treatment of wounds.BACKGROUND OF THE INVENTION[0003]In mammals, dermal injury triggers an organized complex cascade of cellular and biochemical events that result in a healed wound. Wound healing is a complex dynamic process that results in the restoration of anatomic continuity and function: an ideally healed wound is one that has returned to normal anatomic structure, function, and appearance. A typical wound heals via a model consisting of four stages—‘exudative’ phase, proliferative phase, reparati...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61L15/44A61L15/56A61L15/60
CPCA61L2300/406A61L15/60A61L15/46A61K9/06A61K49/0054A61L15/56A61L15/44A61K49/0073A61K49/0043A61K49/0056A61L2300/442A61L2300/414A61K49/0021A61L15/42A61P17/02A61F13/00063A61K31/496A61K38/1825A61K38/1858A61K45/06A61L15/38G01N33/573A61L2300/254
Inventor BALLAMY, LUCY
Owner CONVATEC TECH INC
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