15 results about "Matrix metalloproteases" patented technology

The invention discloses a serum protein marker combination for detecting cerebral microhemorrhage, and belongs to the field of biological medicine detection.The serum protein marker combination comprises four serum proteins including matrix metalloproteinase 3, fibronectin-like extracellular matrix protein 1 containing epidermal growth factors, a metalloproteinase inhibiting factor 1 and uromodulin; through efficient and accurate serum protein marker combination, the defects that brain function magnetic resonance SWI examination is high in cost and long in time consumption, operation depends on high-level imaging physicians and the like are overcome, an innovative tool is provided for rapid, accurate and non-invasive screening and auxiliary diagnosis of cerebral microhemorrhage, and wide clinical application prospects are achieved.

Methods of decreasing shedding of CD28, decreasing soluble CD28 levels, treating cancer and improving immunotherapies comprising inhibiting matrix metalloproteases are provided. Methods of producing agents for performance of the methods of the invention are also provided.

The invention provides a temperature-sensitive hydrogel for resisting bacteria and promoting repair and a preparation method thereof, the temperature-sensitive hydrogel comprises PEG-NB, a matrix metalloproteinase degradable peptide cross-linking agent, gelatin, indocyanine green and levofloxacin, and the temperature-sensitive hydrogel is prepared by photo-crosslinking. According to the temperature-sensitive hydrogel provided by the invention, the structure and the performance of the hydrogel are optimized through the synergistic effect of the gelatin and the PEG-NB, and the mechanical strength, the biocompatibility, the degradability and the like of the hydrogel are remarkably improved; meanwhile, the photo-thermal antibacterial function of the photo-thermal agent indocyanine green and the chemical antibacterial function of levofloxacin are integrated, and near-infrared light irradiation is combined, so that the antibacterial and skin repair promoting effects of the hydrogel are synergistically improved.

This invention discloses a Bifidobacterium that promotes collagen production and its applications, belonging to the field of microbial technology. The Bifidobacterium animalis BBS 01 provided by this invention can promote the expression levels of collagen synthesis-related genes and matrix metalloproteinase inhibitor genes, and reduce the expression levels of matrix metalloproteinase-related genes, thereby promoting collagen production. The Bifidobacterium animalis BBS 01 can be used to prepare edible nutrients or skin nutrients.

The present application relates to the field of biological medicine, and more particularly to a nano-composite system for treating vitiligo, an intelligent controlled-release microneedle patch and a preparation method thereof, wherein the nano-composite system contains enzyme-responsive micelles and polyethylene glycol modified polydopamine nanoparticles; the enzyme-responsive micelles are formed by glyceryl monostearate encapsulating ginseng root-derived exosomes; and the polyethylene glycol modified polydopamine nanoparticles are formed by covalent connection of a polydopamine core and methoxy-polyethylene glycol-amine. Compared with the prior art, the nano-composite system of the present application can realize the spatiotemporal programmed release of drugs in response to the pathological microenvironment, wherein the polydopamine nanoparticles provide instant antioxidant effect, and the exosome micelles trigger release under the condition of overexpression of matrix metalloproteinase-9, thereby synergistically exerting anti-inflammatory and promoting pigment regeneration effects; animal experiments have proved that the patch can effectively reverse the white spots and realize the recoloring of hair and skin. The microneedle patch thus prepared can realize transdermal self-administration, is convenient and painless.

The application discloses a preparation method and application of a dual-immune checkpoint inhibitor modified glycosyl nanoparticle, relates to the technical field of biological medicine, and comprises (a) a core-shell structure nano carrier formed by self-assembly of amphiphilic quaternary ammonium chitosan oligosaccharide, wherein the nano carrier is loaded with a dabrafenib; (b) a PD-1 / PD-L1 pathway blocking polypeptide AUNP-12, which is coupled to the surface of the nano carrier through a covalent bond; and (c) a CTLA-4 pathway blocking antibody alpha-CTLA-4, which is coupled to the surface of the nano carrier through a Schiff base reaction; wherein the amphiphilic quaternary ammonium chitosan oligosaccharide is composed of a hydrophilic quaternary ammonium chitosan oligosaccharide segment, a hydrophobic vitamin E succinate segment and an enzyme-sensitive polypeptide GPLGVRGDG connecting the two segments and capable of being specifically cut by matrix metalloproteinase 2; the dual-immune checkpoint inhibitor modified glycosyl nanoparticle provided by the application has good biological tissue compatibility, and the dual-immune checkpoint inhibitor modified glycosyl nanoparticle (LTHAC) has strong killing effect and targeting capacity on B16F10 melanoma cells with high expression of PD-L1 and abnormal activation of a MAPK pathway.

Disclosed is an in vitro method for assessing whether a human patient suffering from periodontitis has mild periodontitis or advanced periodontitis. The method is based on the insight to determine a selection of two biomarker proteins. Accordingly, in a sample of saliva a patient suffering from periodontitis, the concentrations are measured of the proteins Pyruvate Kinase (PK) and at least one of Matrix metalloproteinase-9 (MMP9), S100 calcium-binding protein A8 (S100A8), and Hemoglobin subunit beta (Hb-beta); or of the proteins Matrix metalloproteinase-9 (MMP9) and at least one of S 100 calcium-binding protein A8 (S100A8) and S100 calcium-binding protein A9 (S100A9). Based on the concentrations as measured, a value is determined reflecting the joint concentrations for said proteins. This value is compared with a threshold value reflecting in the same manner the joint concentrations associated with advanced periodontitis. The comparison allows assessing whether the testing value is indicative of the presence of advanced periodontitis or of mild periodontitis in said patient. Thereby, typically, a testing value reflecting a joint concentration below the joint concentration reflected by the threshold value is indicative for mild periodontitis in said patient, and a testing value reflecting a joint concentration at or above the joint concentration reflected by the threshold value, is indicative for advanced periodontitis in said patient.

Disclosed herein are means, methods, and compositions of matter useful for stimulation of elastin / collagen production and suppression of matrix metalloprotease activity. The disclosure further provides compositionally useful preparations derived from regenerative cells that have been manipulated for optimum growth factor and anti-aging properties. In one embodiment cellular mixtures derived from regenerative cells contacted with activating signals. Said activating signals include stimulation of toll like receptors and other "danger" sensing molecules. In one embodiment regenerative factors are harvested from stem cells and compounded into composition preparations.

This invention belongs to the field of electrochemical analysis technology and relates to an electrochemiluminescence peptide sensing method for detecting matrix metalloproteinases, comprising: 1) mixing a peptide chain solution, an iridium metal complex solution, and a magnetic microparticle MB@SA suspension, adding a protease standard solution, and incubating in one pot; 2) magnetically enriching the magnetic bead complex on the electrode surface and measuring the electrochemiluminescence intensity; 3) changing the volume of the protease standard solution to obtain magnetic bead complexes after reacting with different concentrations of protease and measuring the electrochemiluminescence intensity; obtaining a standard regression equation with protease concentration as the abscissa and the change in electrochemiluminescence intensity as the ordinate; 4) taking the test solution to obtain the corresponding electrochemiluminescence intensity, substituting it into the standard regression equation to obtain the protease concentration. This invention has the advantages of mild probe synthesis conditions, rapid labeling, high sensitivity of the detection method, and ease of operation, realizing rapid, sensitive, and highly selective determination of matrix metalloproteinases.

A lipid nano drug delivery system targeting a brain lesion and a preparation method and application thereof. The drug delivery system comprises a lipid, a delivery drug, and a functional penetrating peptide, and the functional penetrating peptide is formed by covalently connecting a peptide chain linking a nanocarrier end, an arginine-rich penetrating peptide, a matrix metalloproteinase-9 sensitive peptide, and a polyanion inhibitory peptide. The lipid nano drug delivery system can be used for targeting the brain lesion and realizing mitochondrial enrichment by means of modification of the functional penetrating peptide. The repair of mitochondria is realized by encapsulating peptide drug cyclosporin A by means of a lipid nanoparticle core by utilizing a dilution-induced precipitation technique, thereby solving the problems that current cyclosporin A is difficult to effectively reach a brain lesion and the therapeutic window is small, and improving the ability to repair cells around the brain lesion with a small administration dose.

The present disclosure relates to nucleic acid-peptide-nucleic acid conjugate molecules and to methods for synthesizing nucleic acid-peptide-nucleic acid conjugate molecules. In some embodiments, a method for synthesizing a nucleic acid-peptide-nucleic acid conjugate molecule using proximity-enhanced synthesis includes covalently linking a peptide with a first nucleic acid strand via a first reaction, hybridizing the first nucleic acid strand with a second nucleic acid strand to bring the second nucleic acid strand in proximity to the peptide, and covalently linking the peptide with the second nucleic acid strand via a second reaction to provide the nucleic acid-peptide-nucleic acid conjugate molecule. In some embodiments, the peptide of the nucleic acid-peptide-nucleic acid conjugate molecule is a substrate for cleavage by an enzyme, such as matrix metalloproteinase-8 (MMP-8). Exemplary applications of the nucleic acid-peptide-nucleic acid conjugate molecule for drug delivery, molecular assembly of hybrid structures, and constraining the peptide to a biologically active conformation are also disclosed.

This invention discloses a novel application of walnut bark extract. The invention employs a complex enzymatic hydrolysis combined with gradient solvent extraction, supplemented by macroporous resin purification technology, to obtain a walnut bark extract enriched with polyphenolic compounds. It has been demonstrated that this extract can increase the elastin content in human dermal fibroblasts, inhibit the expression of various types of matrix metalloproteinases, and has the effects of improving skin firmness and anti-photoaging. Therefore, it can be applied in the preparation of cosmetics with firming and anti-photoaging functions.

The invention provides an intelligent drug delivery system for treating osteoarthritis as well as a preparation method and application thereof, the intelligent drug delivery system is a drug-loaded micelle of which the surface is covalently coupled with a virus glycoprotein mimic peptide, and the hydrodynamic diameter is 16 + / -5 nm; the sequence of the mimic peptide comprises a type II collagen adhesion domain and a matrix metalloproteinase 13 specific lysis domain, and the mimic peptide can simulate a dual action mechanism of a virus: firstly, the mimic peptide is non-specifically adhered to a cartilage matrix to realize long-acting retention, and then the mimic peptide is specifically activated in a microenvironment in which pathological cells overexpress MMP13, a cell penetrating peptide is exposed, and efficient intracellular delivery of a drug is driven. The technical bottlenecks of short drug residence time, poor cartilage penetrability and lack of lesion cell targeting in the traditional treatment method are solved, the breakthrough from cartilage targeting to precise lesion cartilage cell targeting is realized, cartilage degeneration can be obviously delayed, symptoms can be improved, and excellent curative effects and clinical transformation prospects are shown.

Methods of inhibiting expansion of cerebral contusion, inhibiting secondary hemorrhage and capillary fragmentation in the brain, reducing pericontusional edema and hemorrhage size in the brain, decreasing water content in a CNS tissue, inhibiting disruption of the blood-brain barrier, inhibiting CNS contusion progression and improving post-contusion motor function, inhibiting microvascular impairment caused by endothelial cell swelling and fragmentation, inhibiting extravasation of blood into the brain parenchyma of a subject, inhibiting endothelial cell breakdown in a brain tissue, inhibiting extravasation of endovascular fluids into the brain's interstitium, decreasing vasogenic edema as measured by T2 flair magnetic resonance imaging, and decreasing matrix metalloprotease concentration in a CNS tissue, by administering a SUR1-TRPM4 channel inhibitor alone or in combination with one or more drugs or agents.

The present application relates to a kind of whole-cell biosensors of E. coli surface display, for realizing the high sensitivity detection of multiple proteases.The sensor is displayed on the surface of E. coli by synthetic biology method and includes anchor module, protease-specific cleavage peptide and fluorescent reporter module of recombinant fusion protein.When target protease exists, its specific cleavage peptide causes fluorescent reporter module to dissociate from cell surface, and fluorescence signal is weakened, and the degree of signal attenuation is positively correlated with protease concentration and activity.The detection sensitivity of the present application is high, and the detection limit of matrix metalloproteinase-2, thrombin and other proteases reaches the level of femtogram per milliliter, which is more than three orders of magnitude higher than traditional methods;It is simple to operate, only needs to be added sample-incubation-detection three steps, and the cost of single detection is less than 0.1 dollar;It can directly detect protease activity in complex biological matrix without expensive antibody, and has good clinical application and large-scale screening potential.