4 results about "Matrix metalloproteases" patented technology

This invention discloses a Bifidobacterium that promotes collagen production and its applications, belonging to the field of microbial technology. The Bifidobacterium animalis BBS 01 provided by this invention can promote the expression levels of collagen synthesis-related genes and matrix metalloproteinase inhibitor genes, and reduce the expression levels of matrix metalloproteinase-related genes, thereby promoting collagen production. The Bifidobacterium animalis BBS 01 can be used to prepare edible nutrients or skin nutrients.

The present disclosure relates to nucleic acid-peptide-nucleic acid conjugate molecules and to methods for synthesizing nucleic acid-peptide-nucleic acid conjugate molecules. In some embodiments, a method for synthesizing a nucleic acid-peptide-nucleic acid conjugate molecule using proximity-enhanced synthesis includes covalently linking a peptide with a first nucleic acid strand via a first reaction, hybridizing the first nucleic acid strand with a second nucleic acid strand to bring the second nucleic acid strand in proximity to the peptide, and covalently linking the peptide with the second nucleic acid strand via a second reaction to provide the nucleic acid-peptide-nucleic acid conjugate molecule. In some embodiments, the peptide of the nucleic acid-peptide-nucleic acid conjugate molecule is a substrate for cleavage by an enzyme, such as matrix metalloproteinase-8 (MMP-8). Exemplary applications of the nucleic acid-peptide-nucleic acid conjugate molecule for drug delivery, molecular assembly of hybrid structures, and constraining the peptide to a biologically active conformation are also disclosed.

This invention discloses a novel application of walnut bark extract. The invention employs a complex enzymatic hydrolysis combined with gradient solvent extraction, supplemented by macroporous resin purification technology, to obtain a walnut bark extract enriched with polyphenolic compounds. It has been demonstrated that this extract can increase the elastin content in human dermal fibroblasts, inhibit the expression of various types of matrix metalloproteinases, and has the effects of improving skin firmness and anti-photoaging. Therefore, it can be applied in the preparation of cosmetics with firming and anti-photoaging functions.

The present application relates to a kind of whole-cell biosensors of E. coli surface display, for realizing the high sensitivity detection of multiple proteases.The sensor is displayed on the surface of E. coli by synthetic biology method and includes anchor module, protease-specific cleavage peptide and fluorescent reporter module of recombinant fusion protein.When target protease exists, its specific cleavage peptide causes fluorescent reporter module to dissociate from cell surface, and fluorescence signal is weakened, and the degree of signal attenuation is positively correlated with protease concentration and activity.The detection sensitivity of the present application is high, and the detection limit of matrix metalloproteinase-2, thrombin and other proteases reaches the level of femtogram per milliliter, which is more than three orders of magnitude higher than traditional methods;It is simple to operate, only needs to be added sample-incubation-detection three steps, and the cost of single detection is less than 0.1 dollar;It can directly detect protease activity in complex biological matrix without expensive antibody, and has good clinical application and large-scale screening potential.