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Method for preparing perylenequinone pigment under catalysis of Shiraia bambusicola

A technology for catalyzing preparation and bamboo yellow fungus is applied in the field of bioengineering, and can solve the problems of undeveloped research, inability to apply industrialized production, and low yield of perylene quinone pigments.

Inactive Publication Date: 2009-12-16
江苏竹红生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] However, the yields of the perylenequinone pigments reported above are low and unstable, and no follow-up research has been carried out, so they cannot be applied to industrial production.

Method used

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  • Method for preparing perylenequinone pigment under catalysis of Shiraia bambusicola
  • Method for preparing perylenequinone pigment under catalysis of Shiraia bambusicola
  • Method for preparing perylenequinone pigment under catalysis of Shiraia bambusicola

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Liquid culture of bamboo yellow bacteria, the medium composition is: glucose 40g / L, yeast extract 2g / L, diammonium hydrogen phosphate 2g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.5g / L, 300L air lift type The fermenter has a liquid filling capacity of 70%, a ventilation rate of 1:1.5 v / v / m, a culture time of 56 hours, and about 220 g of wet thalli can be obtained per liter of fermented liquid. Cells were treated as previously described. Put the treated cells into the reaction system, the reaction mixture contains 60g / L cell suspension, 5g / L cinnamic acid, and 50mM potassium phosphate buffer (pH7.0). The reaction started after adding the cell suspension, and the reaction was stirred at 30° C. for 6 h. The total perylenequinone pigment in the final reaction body is 2g / L, and the bacterial cells in the initial stage of the reaction contain 0.2g / L of perylenequinone pigment, and the calculated conversion rate is 9.7%. Among them, hypocrellin A accounts fo...

Embodiment 2

[0055] Somatic cells of bamboo yellow bacteria are cultured in liquid state, and the medium composition is: sucrose 40g / L, yeast extract 2g / L, diammonium hydrogen phosphate 2g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.5g / L. At the initial stage of fermentation, 3 g / L of cinnamic acid was added at one time. 500L mechanical agitation fermenter, ventilation rate 1:0.4v / v / m, stirring speed 150r / min, cultivation time 72 hours, about 191g of wet bacteria can be obtained per liter of fermentation broth. Under this condition, the yield of perylenequinone pigment in the fermentation broth can reach 1.5g / L. Among them, hypocrellin A accounts for 91% of the total pigment amount, and hypocrellin B is 5%. As a control, 0.8 g / L of perylenequinone pigment could be produced without adding cinnamic acid under the same fermentation conditions. The conversion was therefore calculated to be 6.3%.

Embodiment 3

[0057] Liquid culture bacterial cells (strain SUPER-H168): fructose 40g / L, peptone 2g / L, corn steep liquor powder 20g / L, diammonium hydrogen phosphate 2g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 0.5g / L L, compound A5g / L, pH6.0. 300L air-lift fermenter, liquid volume 70%, ventilation rate 1: 1.5v / v / m, culture time 72h, fresh bacteria can be obtained per liter of fermentation broth about 231g. Under this condition, the yield of perylenequinone pigment can reach 2.9g / L. Among them, hypocrellin A accounts for 90% of the total pigment amount, and hypocrellin B is 6%. As a control, 0.6 g / L of perylenequinone pigment could be produced without compound A under the same fermentation conditions. The conversion was therefore calculated to be 26.3%. Compound A:

[0058]

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Abstract

The invention discloses a method for preparing a perylenequinone pigment under the catalysis of Shiraia bambusicola and belongs to the technical field of bioengineering. In the method, naphthoquinone, cinnamic acid and the like which form a substrate are used to prepare the perylenequinone pigment under the catalysis of the Shiraia bambusicola. The catalytic process is implemented by: A), adding the substrate into culture system in a cell liquid culture process, wherein the perylenequinone pigment yield is up to 2.9g / L and the transformation rate is up to 9.7 percent; or B), placing cells obtained by liquid fermentation culture in a reaction system having the substrate, wherein the perylenequinone pigment yield is up to 35.3g / L and the transformation rate is up to 39.7 percent; or C), adding the substrate into a culture system in a cell solid culture process, wherein the perylenequinone pigment yield is up to 1.5g / 100g and the transformation rate is up to 46 percent. The method adopts a perylenequinone pigment-producing Shiraia bambusicola strain and specific substrate transformation to produce hypocrellin, is high in transformation rate, low in cost, less in step and applicable to industrial production and has excellent industrial application prospect.

Description

technical field [0001] The invention relates to the preparation of perylene quinone pigments by using the whole cell catalytic substrate of bamboo yellow fungus, belonging to the technical field of bioengineering. The invention relates to a method for preparing a perylene quinone pigment with a catalyzed substrate of bamboo yellow fungus, and the invention is of great significance for the industrialized production of the perylene quinone pigment. Background technique [0002] Some fungi in nature can produce pigments with perylenequinones. In recent years, the research reports mainly focus on the separation and extraction, photodynamic properties and chemical structure analysis of hypocretin, cercosporin and hypericin, etc., but there is no report on their whole-cell catalytic synthesis. Hypocretin has a series of characteristics such as good stability, no obvious side effects when taken orally, fast metabolism, soluble in many organic solvents and insoluble in water. As a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/66C12R1/645
Inventor 蔡宇杰廖祥儒梁晓辉魏兆媛丁彦蕊孟强李枝玲王亚辉张大兵
Owner 江苏竹红生物科技有限公司
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