20 results about "Transformed cell" patented technology

The present invention describes mRNA usage improving and / or translation-enhancing nucleic acid sequences, nucleic acid constructs comprising such sequences, and host cells comprising such nucleic acid constructs. The invention further pertains to a method for expressing a protein of interest in a cell or organism using such nucleic acid sequences, as well as their uses for increasing integration of such nucleic acid construct into a genome, for enhancing mRNA usage and / or translation of a recombinantly expressed polypeptide, and for increasing the number of transformants upon transformation of a cell with such nucleic acid construct.

Provided are methods of selecting a transformed cell, including methods comprising delivering an inhibitor of a protein to a plurality of cells, exposing the plurality of cells to a compound, wherein the protein (if present and active) converts the compound to a toxic compound, and selecting viable cells from the plurality of cells not killed by exposure to the compound.

PIGGYBAC® transposases engineered to increase stability in a cell. The engineered PIGGYBAC® transposases are useful for stably transforming cells, cell line development, genome modification, and improving titer of recombinant proteins, among other uses.

The application discloses a food-like lactobacillus double-plasmid multi-gene editing system based on CRISPR / Cas9 technology and application thereof. The food-like lactobacillus double-plasmid multi-gene editing system comprises (1) a plasmid pCRI01 comprising the following elements: a Cas9 protein coding gene; a temperature-sensitive replicon pSC101; an sgRNA expression module targeting the replicon of the plasmid pCRI02, which is controlled by an inducible promoter Pnis; an ampicillin resistance; and (2) a plasmid pCRI02 comprising the following elements: at least one sgRNA expression module driven by a P23 promoter; a lambda-Red recombinase system comprising Exo, Beta and Gam genes; an erythromycin resistance marker gene; and a kanamycin resistance gene. The multi-gene editing system provided by the application is a small-size pCRI01 and pCRI02 double-plasmid system, and the number of transformed cells after resistance screening is significantly improved; and the gene editing efficiency mediated by the pCRI plasmid is greatly improved with the assistance of the lambda-Red recombinase system.

A method of producing a transformed cell or plant body of maize of the present invention includes overexpressing, in a cell or plant body of maize, 1) a nucleic acid encoding an amino acid sequence of SEQ ID NO: 2, or a nucleic acid encoding a polypeptide including an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 2, the polypeptide having a function of promoting cell division of maize, or 2) a nucleic acid encoding an amino acid sequence of SEQ ID NO: 15, or a nucleic acid encoding a polypeptide including an amino acid sequence having at least 95% sequence identity with the amino acid sequence of SEQ ID NO: 15, the polypeptide having a function of promoting cell division of maize, the overexpressing being controlled by a promoter including a 35S promoter of Cauliflower mosaic virus.

A monoclonal antibody is provided which binds to a human CC chemokine receptor 1 (CCR1) and inhibits activation of the human CCR1, or an antibody fragment thereof. The monoclonal antibody binds to an extracellular region of a human CCR1 and inhibits activation of the human CCR1 by a human CC chemokine ligand 15 (CCL15). An antibody fragment thereof, a hybridoma producing the antibody, a nucleic acid having a nucleotide sequence encoding the antibody or the antibody fragment, a transformant cell containing a vector containing the nucleic acid, a method for producing the antibody or the antibody fragment using the hybridoma or the transformant cell; a therapeutic agent and a diagnostic agent containing the antibody or the antibody fragment, and a method for treating and diagnosing a CCR1-related disease using the antibody or the antibody fragment are also provided.

This invention provides transformed cells for the production of transformed replica pigs for xenotransplantation. [Solution] This invention relates to a transformed replica pig from which the heterologous antigens GGTA1, CMAH, iGb3s, β4GalNT2, and β2M genes have been removed from a PERV Envelope C-negative base, and a method for producing the same. The transformed replica pig according to the present invention can overcome hyperacute and antigen-antibody-mediated immune rejection reactions and T-cell-mediated immune rejection reactions without causing the transfer of porcine endogenous retroviruses that occur in xenologous organ transplantation, and can be usefully utilized as a donor animal for interspecies organ and cell transplantation.

The present invention relates to: an expression cassette comprising a scaffold / matrix attachment region (S / MAR); an expression vector for expressing a target protein, comprising the expression cassette; a transformant cell obtained by introducing the expression vector into a host cell; and a method for producing a target protein, comprising the step of culturing the transformant cell.

The present invention provides a method for producing a target protein by culturing cells, the method being capable of stably maintaining a target plasmid without using an antibiotic. The present invention relates to a method for producing a target substance, the method including a step for culturing cells that are transformed with a target plasmid (A), wherein: each of the transformed cells includes the target plasmid (A) and a temperature-sensitive plasmid (B) and are obtained by a method that includes a step for culturing cells in each of which the function of an essential gene on the chromosome has been reduced at a temperature at which the temperature-sensitive plasmid (B) is eliminated; the target plasmid (A) includes the essential gene and a target substance gene and does not include an antibiotic-resistant gene; and the temperature-sensitive plasmid (B) includes the essential gene and the antibiotic-resistant gene.

The present application provides a cell culture sheet. The cell culture sheet according to an embodiment of the present application is realized as including a fiber web having a three-dimensional network structure formed by accumulation of support fibers having an average diameter of 1.5 μm or less, a basis weight of 1 to 15 g / m 2 2; and a functional coating layer covering at least the support fibers exposed on one surface of the fiber web and having a function of promoting one or more of cell attachment, migration, proliferation, and differentiation. Thus, due to the high specific surface area and the surface morphology suitable for cells, cell attachment can be improved, the attached cells can be stably supported, cells can be cultured in a highly concentrated manner with high culture efficiency, and the cells can be cultured and recovered without forming a thin film or the like. In addition, compared to cells cultured by conventional culture, small-sized cells can be obtained, and thus, it is very advantageous to culture, proliferate, and renew fresh, healthy, and non-cell-transformed cells. Furthermore, excellent effects are obtained in differentiation and proliferation of stem cells differentiated into specific target cells.

The invention discloses a high-light-resistant algal strain high-speed screening method and system based on micro-fluidic single cell sorting, and relates to the field of bioengineering.The high-light-resistant algal strain high-speed screening method comprises the steps that haematococcus free cells in the logarithmic phase are taken and added into a double-fluorescence probe mixed solution, and incubation is conducted in a dark place to obtain a haematococcus solution; bBM sheath fluid and haematococcus fluid are introduced into a sample introduction area of the micro-fluidic chip, the BBM sheath fluid and the haematococcus fluid are focused by the sample introduction area and then enter a highlight stress area, highlight treatment is carried out to induce motile cells to be transformed into spores, and the transformed cells enter a detection area. Through in-situ high light stress and single cell continuous sorting of the micro-fluidic chip, the sorting efficiency is high, and the screening period is greatly shortened; three parameters of chlorophyll fluorescence, astaxanthin fluorescence and forward scattering light are adopted for cooperative detection, and a threshold value which is quantitatively set based on a control group is combined, so that the proportion of a false positive strain is reduced to be less than 5%, and the screening accuracy of a double-superior strain is greatly improved; the microfluidic chip is extremely low in single reagent consumption, greatly reduces the screening cost of single strains, and is easy for large-scale popularization and application.

The present invention relates to a highly active transposase protein for a transposon system and its uses. By improving the activity of the transposase, genes can be delivered effectively, which can be useful for developing genome-modified cell lines that express various genes. In addition, when T cells are transformed using the highly active transposase of the present invention, cytotoxic T cells (CD8) that have anti-cancer effects can be generated. + The proportion of T cells increases, and T cells persist in the body. CM (Central memory T cell), T SCM Since the proportion of memory-type T cells (stem cell-like memory T cells) increased, it is expected that TCR-T cells and CAR-T cells with good in vivo persistence can be produced using the transposon system of the present invention.

The present invention pertains to: an antibody or an antibody fragment thereof that binds to human TPOR; a nucleic acid that has a base sequence encoding the antibody or the antibody fragment thereof; a vector containing the nucleic acid; a transformed cell containing the vector; a method for producing the antibody or the antibody fragment thereof; a therapeutic agent containing the antibody or the antibody fragment thereof; and a reagent for detection or a reagent for measurement, containing the antibody or the antibody fragment thereof.

The present invention relates to a transformed cell for producing red blood cells and, more specifically: to the transformed cell for producing red blood cells; and a method for producing and differen

The present disclosure relates to a composition for promoting plant growth containing an YxaL protein and a method for mass-producing an YxaL protein. The YxaL protein may be expressed constitutively and in large amounts in a recombinant transformed cell line. When the YxaL protein is applied to seed soaking treatment, root development is improved, and the expression of plant genes involved in root growth is improved in favor of plant growth. Thus, the YxaL protein may be effectively used to promote plant growth.

The present invention aims to provide a method for immortalizing antibody-producing cells and the immortalized transformed cells obtained by this method. The present invention relates to a method for manufacturing immortalized cells, which involves introducing one or more genes selected from the group consisting of the SV40T antigen gene, the Bcl-2 family gene, and the Myc family gene and the Cyclin D family gene into non-immortalized antibody-producing cells. The present invention also relates to a method for manufacturing immortalized antibody-producing cells, which involves collecting antibody-producing cells from mammals, performing primary culture, and introducing one or more genes selected from the group consisting of the SV40T antigen gene, the Bcl-2 family gene, and the Myc family gene and the Cyclin D family gene into the obtained primary antibody-producing cells to create immortalized antibody-producing cells.

Immunogenic proteins from Pseudomonas aeruginosa as well as nucleic acids, vectors and transformed cells useful for expression of the proteins. Also disclosed are methods for prophylaxis of infection with Pseudomonas aeruginosa using the proteins, nucleic acids, vectors or transformed cell.

Provided are a transformed cell having the ability to produce 2,5-pyridine dicarboxylic acids, and a method for producing 2,5-pyridine dicarboxylic acids using the same. The present invention provides a transformed cell having the ability to produce 2,5-pyridine dicarboxylic acids, the transformed cell being derived from a microbe having the ability to biosynthesize 4-aminobenzoic acids and having the enhanced expression of the following polypeptides (I) and (II): (I) a polypeptide having 4-aminobenzoic acid hydroxylation activity, and (II) a polypeptide having 4-amino-3-hydroxybenzoate 2,3-dioxygenase activity.

The application discloses an in vitro transcription system of a transformed cell of a circular theileria and a construction method thereof, and the in vitro transcription system comprises IVT-EGFP-mRNA, and the structure is 5'UTR-Cla1-Kozak-EGFP-PacI-3'UTR-polyA(120). The mRNA coding the EGFP gene is synthesized by in vitro transcription, and is subjected to capping, purification and agarose gel electrophoresis analysis. Finally, the expression of the target gene in the TaNM1 cell is verified by in vitro transfection test and indirect immunofluorescence technology. The result shows that the green fluorescent protein EGFP is successfully expressed by the in vitro transcription system coding the EGFP, which indicates that the system for transfecting the EGFP gene into the transformed cell TaNM1 of the circular theileria by the in vitro transcription method is successfully established, and the system provides important reference value for the design and research and development of the RNA vaccine of theileria annulata.